Abstract
Thirty-five Staphylococcus aureus strains from auricular infections were isolated. The identification of strains was confirmed by Api ID 32 Staph strips, the antibiotic susceptibility test was performed using ATB Staph kit. PCR assay was used to detect the oxacillin resistance gene (mecA) and the erythromycin genes (ermA, ermB, ermC, msrA and mef). The susceptibility profile of all strains revealed a low resistance level to oxacillin and erythromycin. The PCR results show that 60 % of the strains are mecA positive. The frequency of erythromycin genes was: ermA + 22.8 %, ermB + 45.7, ermC + 17.1, msrA + 28.6. The mef gene was not detected in any strain. No correlations between genotypic and phenotypic methods for the determination of oxacillin and erythromycin resistance was found. However, multiplex PCR technique was shown to be a fast, practical and economic technique for the detection of methicillin-and erythromycin-resistant staphylococci.
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Abbreviations
- ERM:
-
erythromycin
- PCR:
-
polymerase chain reaction
- MRSA:
-
methicillin-resistant S. aureus
References
Almer L.S., Shortridge V.D., Nilius A.M., Beyer J.M., Soni N.B., Bui M.H., Stone G.G., Flamm R.K.: Antimicrobial susceptibility and molecular characterization of community-acquired methicillin-resistant Staphylococcus aureus. Diagn.Microbiol. Infect.Dis. 43, 225–232 (2002).
Ardic N., Ozyurt M., Sareyyupoglu B., Haznedaroglu T.: Investigation of erythromycin and tetracycline resistance genes in methicillin-resistant staphylococci. Internat.J.Antimicrob.Agents 26, 213–218 (2005).
Brun-Buisson C.H.: Les Staphylococcus aureus résistants à la méticilline: evolution et épidémiologie, impact clinique, prévention. Pathol.Biol. 46, 227–234 (1998).
Chaieb K., Touati A., Abbassi M.S., Ben Hassen A., Mahdouani K., Bakhrouf A.: DNA fingerprinting of a multi-resistant coagulase-negative staphylococci isolated from biomaterials in dialysis service. Arch.Med.Res. 3, 953–960 (2006).
Chambers H.F.: Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin.Microbiol.Rev. 10, 781–791 (1997).
De Guisti M., Pacifico L., Tufi D., Panero A., Boccia A., Chiesa C.: Phenotypic detection of nosocomial mecA positive coagulase negative staphylococci from neonates. J.Antimicrob.Chemother. 44, 351–358 (1999).
Del Grosso M., Ianneli F., Messina C., Santagati M., Petrosillo N., Stefani S., Pozzi G., Pantosti A.: Macrolide efflux genes mef(A) and mef(E) are carried by different genetic elements in Streptococcus pneumoniae. J.Clin.Microbiol. 40, 774–778 (2002).
Eady E.A., Ross J.I., Tipper J.L., Walters C.E., Cove J.H., Noble W.C.: Distribution of genes encoding erythromycin ribosomal methylases and an erythromycin efflux pump in epidemiologically distinct groups of staphylococci. J.Antimicrob.Chemother. 31, 211–217 (1993).
Fluit A.D.C., Visser M.R., Schmitz F.: Molecular detection of antimicrobial resistance. Clin.Microbiol.Rev. 14, 836–871 (2001).
Geha D.J., Uhl J.R., Gustaferro C.A., Persing D.H.: Multiplex PCR for identification of methicillin-resistant staphylococci in the clinical laboratory. J.Clin.Microbiol. 32, 1768–1772 (1994).
Jahoda D., Nyč O., Pokorný D., Landor I., Krůta T., Sosna A.: Treatment of orthopedic infections caused by resistant staphylococci. Folia Microbiol. 52, 281–286 (2007).
Layton M.C., Hierholzer W.J. Jr., PATTERSON J.E.: The evolving epidemiology of methicillin-resistant Staphylococcus aureus at a university hospital. Infect.Contr.Hosp.Epidemiol. 16, 12–17 (1995).
Leclercq R.: Mechanisms of resistance to macrolides and lincosamides: nature of the resistance elements and their clinical implications. Clin.Infect.Dis. 34, 482–492 (2002).
Leszczyński P., Weber-Dąbrowska B., Kohutnicka M., Łuczak M., Górecki A., Górski A.: Successful eradication of methicillin-resistant Staphylococcus aureus (MRSA) intestinal carrier status in a healthcare worker — case report. Folia Microbiol. 51, 236–238 (2006).
Lim J.A., Kwon A.R., Kim S.K., Chong Y., Lee K., Choi E.C.: Prevalence of resistance to macrolide, lincosamide and streptogramin antibiotics in Gram-positive cocci isolated in a Korean hospital. J.Antimicrob.Chemother. 49, 489–495 (2002).
Lina G., Quaglia A., Reverdy M.E., Leclercq R., Vandenesch F., Etienne J.: Distribution of genes encoding resistance to macrolides, lincosamides, and streptogramins among staphylococci. Antimicrob.Agents Chemother. 43, 1062–1066 (1999).
Marshall S.A., Wilke W.W., Pfaller M.A., Jones R.N.: Staphylococcus aureus and coagulase-negative staphylococci from blood stream infections: frequency of occurrence, antimicrobial susceptibility, and molecular (mecA) characterization of oxacillin resistance in the SCOPE program. Diagn.Microbiol.Infect.Dis. 30, 205–214 (1998).
Martineau F., Picard J.F., Lansac N., Menard C., Roy H.P., Ouellette M., Bergeron G.M.: Correlation between the resistance genotype determined by multiplex PCR assays and the antibiotic susceptibility patterns of Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob.Agents Chemother. 44, 231–238 (2000).
Novotná G., Spížek J., Janata J.: In vitro activity of telithromycin and quinupristin/dalfopristin against methicillin-resistant coagulase-negative staphylococci with defined resistance genotypes. Folia Microbiol. 52, 593–599 (2007).
Pfaller M.A., Jones R.N., Doern G.V., Sader H.S., Kugler K.C., Beach M.L.: Survey of blood stream infections attributable to Gram-positive cocci: frequency of occurrence and antimicrobial susceptibility of isolates collected in 1997 in the United States, Canada, and Latin America from the SENTRY Antimicrobial Surveillance Program. SENTRY Participants Group. Diagn. Microbiol.Infect.Dis. 33, 283–297 (1999).
Raimundo O., Heussler H., Bruhn J.B., Suntrarachun S., Kelly N., Deighton M.A., Garland S.M.: Molecular epidemiology of coagulase-negative staphylococcal bacteraemia in a newborn intensive care unit. J.Hosp.Infect. 51, 33–42 (2002).
Ross J.I., Eady E.A., Cove J.H., Cunliffe W.J., Baumberg S., Wootton J.C.: Inducible erythromycin resistance in staphylococci is encoded by a member of the ATP-binding transport super-gene family. Mol.Microbiol. 4, 1207–1214 (1990).
Ross J.I., Eady E.A., Cove J.H., Baumberg S.: Identification of a chromosomally encoded ABC-transport system with which the staphylococcal erythromycin exporter MsrA may interact. Gene 153, 93–98 (1995).
Sekiguchi J., Fujino T., Konosaki H., Nishimura H., Kawana A., Kudo K., Kondo T., Yazaki Y., Kuratsuji T., Yoshikura H., Kirikae T.: Prevalence of erythromycin-, tetracycline-, and aminoglycoside-resistance genes in methicillin-resistant Staphylococcus aureus in hospitals in Tokyo and Kumamoto. Japan J.Infect.Dis. 57, 74–77 (2004).
Strommenger B., Kettlitz C., Werner G., Witte W.: Multiplex PCR assay for simultaneous detection of nine clinically relevant antibiotic resistance genes in Staphylococcus aureus. J.Clin.Microbiol. 41, 4089–4094 (2003).
Sutcliffe J., Grebe T., Taitkamradt A., Wondrack L.: Detection of erythromycin-resistant determinants by PCR. Antimicrob. Agents Chemother. 40, 2562–2566 (1996).
Weisblum B.: Erythromycin resistance by ribosome modification. Antimicrob.Agents Chemother. 39, 577–585 (1995).
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Zmantar, T., Chaieb, K., Ben Abdallah, F. et al. Multiplex PCR detection of the antibiotic resistance genes in Staphylococcus aureus strains isolated from auricular infections. Folia Microbiol 53, 357–362 (2008). https://doi.org/10.1007/s12223-008-0055-5
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DOI: https://doi.org/10.1007/s12223-008-0055-5