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Regulation of phosphatidylglycerolphosphate synthase in aerobic yeast Kluyveromyces lactis

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Abstract

The KlPGS1 gene encoding phosphatidylglycerolphosphate synthase (PGPS) is essential for the viability and multiplication of Kluyveromyces lactis. Regulation of PGPS expression by factors affecting mitochondrial development (C source, growth phase) and general phospholipid biosynthesis was followed. PGS1 mRNA levels were not altered as cells progressed from the exponential to the stationary phase of growth in glucose. PGS1 mRNA abundance was nearly identical in cells growing in a medium with glucose or glycerol as the sole C source during the different growth phases. Regulation of PGS1 expression by exogenous myo-inositol and choline was not mediated at the transcriptional level, the PGPS activity dropped to 70 % after myo-inositol addition.

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Abbreviations

CL:

cardiolipin

Ino:

myo-inositol

PGPase:

phosphatidylglycerolphosphatase

CLS:

cardiolipin synthase

PG:

phosphatidylglycerol

PGPS:

phosphatidylglycerolphosphate synthase

DAG:

1,2-di-O-acyl-sn-glycerol

PGP:

phosphatidylglycerolphosphate

UAS:

upstream activation sequence

KlPGS1 :

PGS1 gene from Kluyveromyces lactis

ScPGS1 :

PGS1 gene from Saccharomyces cerevisiae

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Authors and Affiliations

  1. Department of Microbiology and Virology, Faculty of Science, Comenius University in Bratislava, 842 15, Bratislava, Slovakia

    E. Tichá, V. Polakovičová & M. Obernauerová

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  1. E. Tichá
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  2. V. Polakovičová
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  3. M. Obernauerová
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Correspondence to M. Obernauerová.

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Tichá, E., Polakovičová, V. & Obernauerová, M. Regulation of phosphatidylglycerolphosphate synthase in aerobic yeast Kluyveromyces lactis . Folia Microbiol 53, 319–324 (2008). https://doi.org/10.1007/s12223-008-0050-x

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  • DOI: https://doi.org/10.1007/s12223-008-0050-x

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