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Vancomycin Loaded Gelatin Microspheres Containing Wet Spun Poly(ε-caprolactone) Fibers and Films for Osteomyelitis Treatment

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Abstract

In this study, it was aimed to develop drug eluting biodegradable and biocompatible bone supportive fibers and films, which can locally be applicable on the infected bone defect area for osteomyelitis treatment. For this purpose, vancomycin (Vm) loaded gelatin (G) microspheres were prepared and dispersed into poly(ε-caprolactone) (PCL) solution and then the suspension was processed as films and fibers by solvent casting and wet spinning techniques, respectively. The mean particle size distribution and morphology of Vm loaded G microspheres were characterized by laser diffraction method and scanning electron microscopy, respectively. In vitro Vm release profiles and release kinetics from microsphere, fiber and film formulations were investigated. In vitro biodegradation properties of fiber and film formulations were examined in both hydrolytic and enzymatic media during 25 days period. The cytotoxicity of Vm eluting films and fibers were tested on L929 cells by MTT assay. Presence of PCL in film and fiber formulations retarded the release of Vm from microspheres and provided long term sustained release. Vm eluting films and fibers exhibited strong antibacterial properties against Staphylococcus aureus and Staphylococcus epidermidis.

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Acknowledgments

This research was supported by Hacettepe University Scientific Research Projects Coordination Unit with project number 014 D10 301 001-718.

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Authors and Affiliations

  1. Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Hacettepe University, Ankara, 06100, Turkey

    Eda Ayse Aksoy, Betul Suyumbike Yagci, Ipek Eroglu, Sukru Ozturk & Kezban Ulubayram

  2. Polymer Science and Technology Division, Institute for Graduate Studies in Science and Engineering, Hacettepe University, Ankara, 06800, Turkey

    Eda Ayse Aksoy, Gulseher Manap & Kezban Ulubayram

  3. Bioengineering Division, Institute for Graduate Studies in Science and Engineering, Hacettepe University, Ankara, 06800, Turkey

    Sukru Ozturk & Kezban Ulubayram

  4. Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Hacettepe University, Ankara, 06100, Turkey

    Melike Ekizoglu

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  1. Eda Ayse Aksoy
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  2. Betul Suyumbike Yagci
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  3. Gulseher Manap
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Correspondence to Eda Ayse Aksoy.

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Aksoy, E.A., Yagci, B.S., Manap, G. et al. Vancomycin Loaded Gelatin Microspheres Containing Wet Spun Poly(ε-caprolactone) Fibers and Films for Osteomyelitis Treatment. Fibers Polym 20, 2236–2246 (2019). https://doi.org/10.1007/s12221-019-9271-7

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  • DOI: https://doi.org/10.1007/s12221-019-9271-7

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