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Fabrication of electropsun PLGA and small intestine submucosa-blended nanofibrous membranes and their biocompatibility for wound healing

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Abstract

In recent decades, tremendous research has focused on the production of nanoscale fibers using synthetic polymers, with the goal of fabricating nanofibrous scaffolds for wound healing. However, the hydrophobicity of such polymers typically hinders attachment and proliferation of the cells. In this study, we combined poly-d,l-lactide-co-glycolide (PLGA) and small intestine submucosa (SIS) to fabricate blended nanofibers for wound healing by electrospinning. PLGA and SIS were dissolved in 1,1,1,3,3,3-hexafluoro isopropanol to produce different weight ratios of PLGA/SIS-blended nanofibrous membranes (NFM). Physicochemical characterization of the electrospun NFM was performed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, water contact angle analysis, degradation test and tensile testing. The PLGA/SIS-blended NFM showed improved hydrophilicity and tensile strength. Better infiltration, attachment and proliferation of rat granulation fibroblasts of PLGA/SIS-blended NFMs compared to PLGA NFMs were identified by morphological differences determined by SEM and a water-soluble tetrazolium salt assay kit. Based on our results, the PLGA/SIS blended NFMs were found to be suitable for use as a potential material for wound dressing.

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Authors and Affiliations

  1. Department of Veterinary Surgery, Konkuk University, Seoul, 05025, Korea

    Kihoon Kim, Hyun Kim, Jaiho Shin, Yongwon Shin & Hwi-Yool Kim

  2. Department of Materials Chemistry and Engineering, Konkuk University, Seoul, 05025, Korea

    Jong Yoon Lee & Young Tai Yoo

Authors
  1. Kihoon Kim
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  2. Jong Yoon Lee
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  3. Hyun Kim
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  4. Jaiho Shin
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  5. Yongwon Shin
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  6. Young Tai Yoo
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  7. Hwi-Yool Kim
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Correspondence to Hwi-Yool Kim.

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Kim, K., Lee, J.Y., Kim, H. et al. Fabrication of electropsun PLGA and small intestine submucosa-blended nanofibrous membranes and their biocompatibility for wound healing. Fibers Polym 18, 231–239 (2017). https://doi.org/10.1007/s12221-017-6936-y

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  • DOI: https://doi.org/10.1007/s12221-017-6936-y

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