Abstract
Electrospinning has been recognized as an efficient technique for the fabrication of neural tissue engineering scaffolds. Many approaches have been developed on material optimization, electrospinning techniques, and physical properties of scaffolds to produce a suitable scaffold for tissue engineering aspects. In this study, structural properties of scaffolds were promoted by controlling the speed of fiber collection without any post-processing. PLGA scaffolds, in two significantly different solution concentrations, were fabricated by the electrospinning process to produce scaffolds with the optimum nerve cell growth in a desired direction. The minimum, intermediate and maximum rate of fiber collection (0.4, 2.4, 4.8 m/s) formed Random, Aligned and Drown-aligned fibers, with various porosities and hydrophilicities. The scaffolds were characterized by fiber diameter, porosity, water contact angle and morphology. Human nerve cells were cultured on fiber substrates for seven days to study the effects of different scaffold structures on cell morphology and proliferation, simultaneously. The results of MTT assay, the morphology of cells and scaffold characterization recommend that the best structure to promote cell direction, morphology and proliferation is accessible in an optimized hydrophilicity and porosity of scaffolds, which was obtained at the collector linear speed of 2.4 m/s.
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J. P. Fisher, A. G. Mikos, and J. D. Bronzino, “Tissue Engineering”, pp.304–317, Taylor & Francis, New York, 2007.
C. V. Blitterswijk, P. Thomsen, A. Lindahl, J. Hubbell, D. Williams, R. Cancedda, J. D. Bruijn, and J. Sohier, “Tissue Engineering”, pp.611–644, Elsevier Academic, Canada, 2008.
S. Ramakrishna, K. Fujihara, W. E. Teo, T. C. Lim, and Z. Ma, “An Introduction to Electrospinning and Nanofibers”, pp.275–340, World Scientific, Singapore, 2005.
D. Liang, B. S. Hsiao, and B. Chu, Adv. Drug Delivery Reviews, 59, 1392 (2007).
H. Cao, T. Liu, and S. Chew, Adv. Drug Delivery Reviews, 61, 1055 (2009).
J. Zhou, C. Cao, and X. Ma, Int. J. Biological Macromolecules, 45, 504 (2009).
J. L. Lowery, N. Datta, and G. C. Rutledge, J. Biomaterials., 31, 491 (2010).
P. Sangsanoh, S. Waleetorncheepsawat, O. Suwantong, P. Wutticharoenmongkol, O. Weeranantanapan, B. Chuenjitbuntaworn, P. Cheepsunthorn, P. Pavasant, and P. Supaphol, Biomacromolecules, 8, 1587 (2007).
E. Schnell, K. Klinkhammer, S. Balzer, G. Brookc, D. Klee, P. Daltonb, and J. Mey, Biomaterials, 28, 3012 (2007).
P. Wutticharoenmongkol, P. Pavasant, and P. Supaphol, Biomacromolecules, 8, 2602 (2007).
Y. Xiong, Y. Zeng, C. Zeng, B. Du, and L. He, J. Biomaterials., 30, 3711 (2009).
H. Tabesh, Gh. Amoabediny, N. Salehi Nik, M. Heydari, M. Yosefard, S. O. Ranaei Siadat, and K. Mottaghy, J. Neurochemistry Int., 54, 73 (2009).
A. Krych, G. Rooney, B. Chen, T. Schermerhorn, and S. Ameenuddin, J. Acta Biomaterialia., 5, 2551 (2009).
M. Moore, J. Friedmanb, E. Lewellync, S. Mantilaa, and S. Krychd, J. Biomaterials., 27, 419 (2006).
L. Yao, S. Wang, W. Cui, B. Du, and R. Sherlock, J. Acta Biomaterialia., 5, 580 (2009).
E. Bible, D. Chau, M. Alexander, J. Price, and K. Shakesheff, Biomaterials, 30, 2985 (2009).
N. Madigan, S. McMahon, T. Brien, M. Yaszemski, and A. Windebank, Respiratory Physiology & Neurobiology, 169, 183 (2009).
Y. Ikada, “Tissue Engineering Fundamentals and Applications”, pp.303–309, Elsevier Academic, Japan, 2008.
B. Wang, Q. Cai, S. Zhang, X. Yang, and X. Deng, J. Mechanical Behavior. Biomed. Mater., 4, 600 (2011).
H. B. Wang, M. E. Mullins, J. M. Cregg, C. W. McCarthy, and R. J. Gilbert, Acta Biomaterialia, 6, 2970 (2010).
F. Yang, R. Murugan, S. Wang, and S. Ramakrishna, Biomaterials., 26, 2603 (2005).
S. Patel, K. Kurpinski, R. Quigley, H. Gao, B. S. Hsiao, M. M. Poo, and S. Li, Nano Lett., 7, 2122 (2007).
D. Gupta, J. Venugopal, M. P. Prabhakaran, V. R. GiriDev, S. Low, A. T. Choon, and S. Ramakrishna, Acta Biomaterialia., 5, 2560 (2009).
Z. X. Meng, Y. S. Wang, C. Ma, W. Zheng, L. Li, and Y. F. Zheng, Mater. Sci. Eng., 30, 1204 (2010).
K. T. Shalumon, N. S. Binulal, N. Selvamurugan, S. V. Nair, D. Menon, T. Furuike, H. Tamura, and R. Jayakumar, Carbohyd. Polym., 77, 863 (2009).
L. Ghasemi-Mobarakeh, M. P. Prabhakaran, M. Morshed, M. H. Nasr-Esfahani, and S. Ramakrishna, Biomaterials, 29, 4532 (2008).
J.M. Deitzel, J. Kleinmeyer, D. Harris, and N. C. Beck Tan, Polymer, 42, 261 (2001).
C. J. Thompson, G. G. Chase, A. L. Yarin, and D. H. Reneker, Polymer, 48, 6913 (2007).
S. D. Vrieze, T. V. Camp, A. Nelvig, B. Hagstrom, P. Westbroek, and K. D. Clerck, J. Mater. Sci., 44, 1357 (2009).
A. Kilic, F. Oruc, and A. Demir, Text. Res. J., 78, 532 (2008).
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Zamani, F., Latifi, M., Amani-Tehran, M. et al. Effects of PLGA nanofibrous scaffolds structure on nerve cell directional proliferation and morphology. Fibers Polym 14, 698–702 (2013). https://doi.org/10.1007/s12221-013-0698-y
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DOI: https://doi.org/10.1007/s12221-013-0698-y