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Effect of PGRs in adventitious root culture in vitro: present scenario and future prospects

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Abstract

Plants, a source of undeviating therapeutic agents, are used as a model system for new synthetic compounds and a taxonomic marker for discovery of novel compounds. The expanding awareness of the health hazards and toxicity associated with the indiscriminate use of synthetic drugs has enhanced the interest in the use of plants and plant-based drugs. The evolving commercial significance of secondary metabolites in recent years has resulted in a great interest in secondary metabolism, particularly in the prospect of varying the production of bioactive plant metabolites by means of tissue culture technology. Adventitious root culture system (AR) provides an alternative approach for the improvement and enhancement of plant-based pharmaceutical compounds. AR serves as a base for conservation of important plant species by reducing pressure on its natural populations. The present review focuses on the application of plant growth regulators (particularly auxins and cytokinins) for the initiation and establishment of adventitious root cultures through various explants in vitro. The production of root biomass could provide bioactive compounds of interest in laboratory conditions. In addition, the root biomass production, accumulation and quantification of secondary metabolites studies in different medicinal plant species have been discussed in the light of different PGRs.

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Abbreviations

2, 4-D:

2, 4-Dichlorophenoxyacetic acid

IAA:

3-Indoleacetic acid

B5:

Gamborg’s medium

BAP:

6-Benzylaminopurine

CGs:

Galloylglucoses

CPT:

Camptothecin

DW:

Dry weight

FW:

Fresh weight

HPLC:

High performance liquid chromatography

IBA:

Indole-3-butyric acid

ISM:

Indian system of medicines

Kin:

Kinetin

LC–MS:

Liquid Chromatography Mass Spectrometry

LS:

Linsmaier and Skoog medium

MS:

Murashige and Skoog medium

N6:

Chu medium

NAA:

α-Naphthalene acetic acid

NMR:

Nuclear magnetic resonance

PVP:

Polyvinylpyrrolidone

TLC:

Thin layer chromatography

AR:

Adventitious roots

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Acknowledgments

The award of SERB, DST, Young Scientist (SB/YS/LS-145/2013) scheme to Dr. Naseem Ahmad by the Department of Science and Technology (DST), Government of India, New Delhi, is greatly acknowledged. The financial assistance in the form of Dr. D. S. Kothari Postdoctoral Fellowship to Dr. Nigar Fatima from University Grants Commission (UGC), Government of India, New Delhi, is also acknowledged. Research support from The Department of Science and Technology (Govt. of India) New Delhi under the DST- FIST (2011) and UGC-SAP (2009) Programme, is also acknowledged.

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202 002, India

    Naseem Ahmad & Mohammad Anis

  2. Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, 202 002, India

    Nigar Fatima & Iqbal Ahmad

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  1. Naseem Ahmad
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  2. Nigar Fatima
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  4. Mohammad Anis
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Correspondence to Naseem Ahmad.

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Ahmad, N., Fatima, N., Ahmad, I. et al. Effect of PGRs in adventitious root culture in vitro: present scenario and future prospects. Rend. Fis. Acc. Lincei 26, 307–321 (2015). https://doi.org/10.1007/s12210-015-0445-y

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