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Research of 1,3-Dihydroxyacetone Production by Overexpressing Glycerol Transporter and Glycerol Dehydrogenase

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Abstract

1,3-Dihydroxyacetone (DHA), a natural ketose, is widely used in the chemical, cosmetic, and pharmaceutical industries. The current method for DHA production is Gluconobacter oxydans (G. oxydans) fermentation, but the high concentration of glycerol in the fermentation broth inhibits cells growth. To overcome this obstacle, in this study, we overexpressed the glycerol transporter (GlpFp) by the use of promoters PtufB, Pgmr, Pglp1, and Pglp2 in G. oxydans 621H. The results show that the glycerol tolerances of strains overexpressing GlpF were all much better than that of the control strain. The glycerol dehydrogenase gene (Gdh) was overexpressed by the promoters PtufB and Pgdh, which increased the DHA titer by 12.7% compared with that of the control group. When GlpF and Gdh genes were co-overexpressed in G. oxydans 621H, the OD600 value of the engineered strains all increased, but the DHA titers decreased in different degrees, as compared with strains that overexpressed only Gdh. This study provides a reference for future research on DHA production.

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Acknowledgements

This work was supported by the Major Research Plan of Tianjin (16YFXTSF00460).

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Author notes

  1. Jinxiu Tan and Xiaona Yang have contributed equally to this work.

Authors and Affiliations

  1. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China

    Jinxiu Tan, Xiaona Yang & Wenyu Lu

  2. Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin, 300072, China

    Wenyu Lu

  3. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), SynBio Research Platform, Tianjin, 300072, China

    Wenyu Lu

Authors
  1. Jinxiu Tan
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  2. Xiaona Yang
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  3. Wenyu Lu
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Correspondence to Wenyu Lu.

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Tan, J., Yang, X. & Lu, W. Research of 1,3-Dihydroxyacetone Production by Overexpressing Glycerol Transporter and Glycerol Dehydrogenase. Trans. Tianjin Univ. 25, 549–558 (2019). https://doi.org/10.1007/s12209-019-00207-w

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  • DOI: https://doi.org/10.1007/s12209-019-00207-w

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