Abstract
Shear stress environment is an important factor in the study of vascular tissue engineering. It is a key issue to improve the adhesion ability of endothelial cells in shear stress environment to format a complete monolayer of endothelial cells on the inner surface of the artificial blood vessel. This study uses electrospinning technology to construct a zein film with nano structure to provide basement membrane for cell retention study. Two parallel plate flow chamber devices are designed to simulate the fluid environment of the blood vessel to compare the adhesion ability of EA.hy926 and L929 cells on the fiber film under shear stress. From the data of cell retention, we find that the shear stress in each hole of 6-hole device is much uniform than that of 18-hole device. This optimized 6-hole device can be used to compare the anti-detaching ability of cells between various surfaces.
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ZOGHBI W A, DUNCAN T, ANTMAN E, et al. Sustainable development goals and the future of cardiovascular health: A statement from the global cardiovascular disease taskforce [J]. Journal of the American Heart Association, 2014, 3(5): e000504.
DEUTSCH M, MEINHART J, ZILLA P, et al. Long-term experience in autologous in vitro endothelialization of infrainguinal ePTFE grafts [J]. Journal of Vascular Surgery, 2009, 49(2): 352–362.
ESLAMI M H, GANGADHARAN S P, BELKIN M, et al. Monocyte adhesion to human vein grafts: A marker for occult intraoperative injury? [J]. Journal of Vascular Surgery, 2001, 34(5): 923–929.
BAGUNEID M, MURRAY D, SALACINSKI H J, et al. Shear-stress preconditioning and tissueengineering-based paradigms for generating arterial substitutes [J]. Biotechnology and Applied Biochemistry, 2004, 39(2): 151–157.
ROSS J J, TRANQUILLO R T. ECM gene expression correlates with in vitro tissue growth and development in fibrin gel remodeled by neonatal smooth muscle cells [J]. Matrix Biology, 2003, 22(6): 477–490.
GRASSL E D, OEGEMA T R, TRANQUILLO R T. A fibrin-based arterial media equivalent [J]. Journal of Biomedical Materials Research Part A: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials, 2003, 66(3): 550–561.
ISENBERG B C, WILLIAMS C, TRANQUILLO R T. Endothelialization and flow conditioning of fibrin-based media-equivalents [J]. Annals of Biomedical Engineering, 2006, 34(6): 971–985.
WANG J Y, FUJIMOTO K, MIYAZAWA T, et al. Antioxidative mechanism of maize zein in powder model systems against methyl linoleate: Effect of water activity and coexistence of antioxidants [J]. Journal of Agricultural and Food Chemistry, 1991, 39(2): 351–355.
PALIWAL R, PALAKURTHI S. Zein in controlled drug delivery and tissue engineering [J]. Journal of Controlled Release, 2014, 189: 108–122.
DONG J, SUN Q S, WANG J Y. Basic study of corn protein, zein, as a biomaterial in tissue engineering, surface morphology and biocompatibility [J]. Biomaterials, 2004, 25(19): 4691–4697.
LIN J T, LI C H, ZHAO Y, et al. Co-electrospun nanofibrous membranes of collagen and zein for wound healing [J]. ACS Applied Materials & Interfaces, 2012, 4(2): 1050–1057.
YAO C, LI Y L, WU F F. Zein nanofibrous membranes as templates for biomineralization of hydroxyapatite crystallites [J]. Polymer Composites, 2013, 34(7): 1163–1171.
VAN KOOTEN T G, SCHAKENRAAD J M, VAN DER MEI H C, et al. Development and use of a parallel-plate flow chamber for studying cellular adhesion to solid surfaces [J]. Journal of Biomedical Materials Research, 1992, 26(6): 725–738.
CHRIST K V, TURNER K T. Methods to measure the strength of cell adhesion to substrates [J]. Journal of Adhesion Science and Technology, 2010, 24(13/14): 2027–2058.
GERSZTEN R E, LIM Y C, DING H T, et al. Adhesion of monocytes to vascular cell adhesion molecule-1-transduced human endothelial cells [J]. Circulation Research, 1998, 82(8): 871–878.
LANE W O, JANTZEN A E, CARLON T A, et al. Parallel-plate flow chamber and continuous flow circuit to evaluate endothelial progenitor cells under laminar flow shear stress [J]. Journal of Visualized Experiments, 2012(59): e3349.
KAWAMOTO Y, NAKAO A, ITO Y, et al. Endothelial cells on plasma-treated segmented-polyurethane: Adhesion strength, antithrombogenicity and cultivation in tubes [J]. Journal of Materials Science: Materials in Medicine, 1997, 8(9): 551–557.
OBI S, YAMAMOTO K, ANDO J. Effects of shear stress on endothelial progenitor cells [J]. Journal of Biomedical Nanotechnology, 2014, 10(10): 2586–2597.
FRANGOS J A, MCINTIRE L V, ESKIN S G. Shear stress induced stimulation of mammalian cell metabolism [J]. Biotechnology and Bioengineering, 1988, 32(8): 1053–1060.
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the Science and Technology Commission Shanghai Municipality (No. 18490740200), the Shanghai Municipal Education Commission (No. ZXGF082101), and the National Key Research and Development Project (No. 2019YFE0101200)
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Shen, N., Zhang, Y. & Wang, J. Parallel Plate Flow Chamber Device for Evaluation of Cell Retention of Electrospun Zein Fiber Film Under Steady Shear. J. Shanghai Jiaotong Univ. (Sci.) 26, 69–75 (2021). https://doi.org/10.1007/s12204-021-2262-z
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DOI: https://doi.org/10.1007/s12204-021-2262-z