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Parametric Analysis for Monitoring 2D Kinetics of Receptor–Ligand Binding

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Abstract

Thermal fluctuation approach is widely used to monitor association kinetics of surface-bound receptor–ligand interactions. Various protocols such as sliding standard deviation (SD) analysis (SSA) and Page’s test analysis (PTA) have been used to estimate two-dimensional (2D) kinetic rates from the time course of displacement of molecular carrier. In the current work, we compared the estimations from both SSA and modified PTA using measured data from an optical trap assay and simulated data from a random number generator. Our results indicated that both SSA and PTA were reliable in estimating 2D kinetic rates. Parametric analysis also demonstrated that such the estimations were sensitive to parameters such as sampling rate, sliding window size, and threshold. These results furthered the understandings in quantifying the biophysics of receptor–ligand interactions.

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Acknowledgments

We thank Dr. R. P. McEver for generous gifts of sLs, CA21, DREG56, PL1, and PL2 proteins, and Dr. Z. Z. Ye for PSGL-1 purification. This work was supported by National Natural Science Foundation of China grants 30730032 and 30730093, National Key Basic Research Foundation of China grant 2006CB910303, National High Technology Research and Development Program of China grant 2007AA02Z306, and Knowledge Innovation Program of CAS grant KJCX2-YW-L08.

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  1. National Microgravity Laboratory and Center for Biomechanics and Bioengineering, Institute of Mechanics, Chinese Academy of Sciences, Beijing, 100190, P.R. China

    Ganyun Sun, Yan Zhang, Bo Huo & Mian Long

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  1. Ganyun Sun
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  2. Yan Zhang
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Correspondence to Mian Long.

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Sun, G., Zhang, Y., Huo, B. et al. Parametric Analysis for Monitoring 2D Kinetics of Receptor–Ligand Binding. Cel. Mol. Bioeng. 2, 495–503 (2009). https://doi.org/10.1007/s12195-009-0079-1

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