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Cyst viability and stress tolerance upon heat shock protein 70 knockdown in the brine shrimp Artemia franciscana

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Cell Stress and Chaperones Aims and scope

Abstract

Females of the brine shrimp Artemia franciscana produce either free-swimming nauplii via ovoviviparous pathway of reproduction or encysted embryos, known as cysts, via oviparous pathway, in which biological processes are arrested. While previous study has shown a crucial role of ATP-dependent molecular chaperone, heat shock protein 70 (Hsp70) in protecting A. franciscana nauplii against various abiotic and abiotic stressors, the function of this protein in diapausing embryos and cyst development, however, remains unknown. RNA interference (RNAi) was applied in this study to examine the role of Hsp70 in cyst development and stress tolerance, with the latter performed by desiccation and freezing, a common method used for diapause termination in Artemia cysts. Hsp70 knockdown was apparent in cysts released from females that were injected with Hsp70 dsRNA. The loss of Hsp70 affected neither the development nor morphology of the cysts. The time between fertilization and cyst release from Artemia females injected with Hsp70 dsRNA was delayed slightly, but the differences were not significant when compared to the controls. However, the hatching percentage of cysts which lacks Hsp70 were reduced following desiccation and freezing. Taken together, these results indicated that Hsp70 possibly plays a role in the stress tolerance but not in the development of diapause-destined embryos of Artemia. This research makes fundamental contributions to our understanding of the role molecular chaperone Hsp70 plays in Artemia, an excellent model organism for diapause studies of the crustaceans.

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Acknowledgments

This paper is dedicated to the late Prof. Dr. Thomas H. MacRae from Dalhousie University, Canada, for his tireless efforts in developing the method to knockdown Hsp70 in Artemia. MTMI was supported by MyBrain 15 Scholarship from the Ministry of Higher Education, Malaysia.

Funding

This work was partially supported by the Fundamental Research Grant Scheme (FRGS) No. 59331 and No. 59578 from the Ministry of Higher Education, Malaysia to YYS, as well as the Sultan Mizan Professiorial Chair Research Fund No. 53264 to PS.

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Authors and Affiliations

  1. Institute of Marine Biotechnology, Universiti Malaysia Terengganu (UMT), 21030, Kuala Nerus, Malaysia

    Mat Taib Mimi Iryani, Patrick Sorgeloos, Muhd Danish-Daniel, Min Pau Tan, Li Lian Wong, Wen Jye Mok & Yeong Yik Sung

  2. Laboratory of Aquaculture and Artemia Reference Center, Department of Animal Sciences and Aquatic Ecology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium

    Patrick Sorgeloos

  3. Department of Fish Health Management and Aquaculture, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya, 60115, Indonesia

    Woro Hastuti Satyantini, Gunanti Mahasri & Yeong Yik Sung

Authors
  1. Mat Taib Mimi Iryani
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  2. Patrick Sorgeloos
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  3. Muhd Danish-Daniel
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  4. Min Pau Tan
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  5. Li Lian Wong
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  7. Woro Hastuti Satyantini
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  8. Gunanti Mahasri
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  9. Yeong Yik Sung
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Correspondence to Yeong Yik Sung.

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Iryani, M.T.M., Sorgeloos, P., Danish-Daniel, M. et al. Cyst viability and stress tolerance upon heat shock protein 70 knockdown in the brine shrimp Artemia franciscana. Cell Stress and Chaperones 25, 1099–1103 (2020). https://doi.org/10.1007/s12192-020-01113-0

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  • DOI: https://doi.org/10.1007/s12192-020-01113-0

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