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Protective manifestation of bacoside A and bromelain in terms of cholinesterases, gamma-amino butyric acid, serotonin level and stress proteins in the brain of dichlorvos-intoxicated mice

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Cell Stress and Chaperones Aims and scope

Abstract

The objective of the study was to evaluate the neuroprotective effects of bacoside A and bromelain against dichlorvos-incited toxicity. Healthy 6–8-week old, male Swiss mice were administered subacute doses of dichlorvos (40 mg/kg bw), bacoside A (5 mg/kg bw) and bromelain (70 mg/kg bw). AChE, BChE, GABA, serotonin and total protein content and their expressions were used for determination of toxic action of dichlorvos. Protective effects of bacoside A and bromelain were evaluated on the same parameters. Exposure to dichlorvos leads to significant decline in activities of AChE (p < 0.01, p < 0.001), BChE (p < 0.05) and GABA (p < 0.01) and total protein levels (p < 0.01). Antioxidant treatment significantly increased the activities of AChE (p < 0.01, p < 0.001), BChE (p < 0.05), GABA (p < 0.01) and total protein level (p < 0.05) compared to those in dichlorvos-treated mice. Overexpression of Hsp 70 protein and underexpression of phosphorylase a and b, catalase SOD and GPx were observed after dichlorvos exposure which suggests the oxidative stress. The results indicate that dichlorvos-induced neuronal damage which results in the generation of molecular expression of proteins is in agreement with the biochemical data ameliorated by bacoside A and bromelain.

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References

  • Anbarsi K, Vani G, Balakrishna K, Devi CS (2006) Effect of bacoside A on brain antioxidant status in cigarette smoke exposed rats. Life Sci 78(12):1378–1384

    Article  Google Scholar 

  • Aridon P, Geraci F, Turturici GD, Amelio M, Savettieri G, Sconzo G (2011) Protective role of heat shock proteins in Parkinson’s disease. Neurodegener Dis 8:155–168

    Article  CAS  PubMed  Google Scholar 

  • Bhattacharya SK, Bhattacharya A, Kumar A, Ghosal S (2000) Antioxidant activity of Bacopa monniera in rat frontal cortex, striatum and hippocampus. Phytother Res 14:174–179

    Article  CAS  PubMed  Google Scholar 

  • Bist R, Bhatt DK (2009) The evaluation of effect of alpha-lipoic acid and vitamin E on the lipid peroxidation, gamma-amino butyric acid and serotonin level in the brain of mice (Mus musculus) acutely intoxicated with lindane. J Neurol Sci 276(1–2):99–102

    Article  CAS  PubMed  Google Scholar 

  • Bist R, Bhatt DK (2010) Augmentation of cholinesterases and ATPase activities in the cerebellum and pons-medulla oblongata, by a combination of antioxidants (resveratrol, ascorbic acid, alpha-lipoic acid and vitamin E), in acutely lindane intoxicated mice. J Neurol Sci 296(1–2):83–87

    Article  CAS  PubMed  Google Scholar 

  • Celik I, Yilmaz Z, Turkoglu V (2009) Hematotoxic and hepatotoxic effects of dichlorvos at sublethal dosages in rats. Inc Environ Toxicol 24:128–132

    Article  CAS  Google Scholar 

  • Chaudhary B, Agrawal S, Bist R (2014) Obliteration in oxidative stress and Ca++ uptake in brain mitochondria leads to impairment of cholinergic system: a possible mechanism underlying neurotoxicity induced by dichlorvos. BBB 2(3):550–564

    CAS  Google Scholar 

  • Ellman GL, Courtney KD, Andres V Jr, Feather-stone RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7:88–95

    Article  CAS  PubMed  Google Scholar 

  • Ericsson C, Nister M (2011) Protein extraction from solid tissues. Methods in Mol Bio 675:307–312

    Article  CAS  Google Scholar 

  • Fernandez CR, Fields A, Richards T, Kaye AD (2003) Anesthetic considerations in patients with Alzheimer’s disease. J Clin Anesth 15:5258

    Article  Google Scholar 

  • Fodale V, Quattrone D, Trecroci C, Caminiti V, Santamaria LB (2006) Alzheimer’s disease and anaesthesia: implications for the central cholinergic system. Br J Anaesth 97:445452

    Article  Google Scholar 

  • Graham LT Jr, Aprison MH (1966) Flurometric determination of aspartate, glutamate and gamma-aminobutyrate in nerve tissue using enzymic methods. Anal Biochem 15:487–497

    Article  CAS  PubMed  Google Scholar 

  • Habashi SA, Moghimi A, Sabouni F, Majd SA (2012) Inhibition of NO production in LPS-stimulated primary rat microglial cells by bromelain. J of Cell and Mol Res 3(2):57–65

    Google Scholar 

  • Jadhav KB, Rajini PS (2009) Evaluation of sublethal effects of dichlorvos upon Caenorhabditis elegans based on a set of end points of toxicity. J Biochem Mol Toxicol 23(1):9–17

    Article  CAS  PubMed  Google Scholar 

  • Janani P, Sivakumari K, Geetha A, Ravisankar B, Parthasarathy C (2010) Chemopreventive effect of bacoside A on N-nitrosodiethylamine induced hepatocarcinogenesis in rats. J Cancer Res Clin Oncol 136:759–770

    Article  CAS  PubMed  Google Scholar 

  • Kaur P, Radotra B, Minz RW, Gill KD (2007) Impaired mitochondrial energy metabolism and neuronal apoptotic cell death after chronic dichlorvos (OP) exposure in rat brain. Neurotoxicol 28(6):1208–1219

    Article  CAS  Google Scholar 

  • Kobayashi H, Yuyama A, Chiba KI (1986) Cholinergic system of brain tissue in rats poisoned with the organophosphate 0,0-demethyl 0-(2,2-dichlorovinyl) phosphate. Toxicol Appl Pharmacol 82:32–39

    Article  CAS  PubMed  Google Scholar 

  • Kumar GP, Khanum F (2012) Neuroprotective potential of phytochemicals. Pharmacogn Rev 6(12):81–90

    Article  PubMed  PubMed Central  Google Scholar 

  • Lauer S, Reichenbach A, Birkenmeier G (2001) Alpha 2-macroglobulin-mediated degradation of amyloid beta 1-42: a mechanism to enhance amyloid beta catabolism. Exp Neurol 167:385–392

    Article  CAS  PubMed  Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) The original method for protein estimation. Biol Chem 193:265

    CAS  Google Scholar 

  • Maslinska D, Lewandowska I, Prokopczyk J (1981) Effect of prolonged acetylcholinesterase inhibition on postnatal brain development in rabbit. I. Level of serotonin in different brain regions. Acta Neuropathol, suppl Berl 7:52–55

    Article  CAS  Google Scholar 

  • Mathew J, Paul J, Nandhu MS, Paulose CS (2009) Bacopa monnieri and bacoside–A for ameliorating epilepsy associated behavioral deficits. Fitoterapia 81(5):315–322

    Article  PubMed  Google Scholar 

  • Pilot MA, Thompson HH, Zara GP (1983) Effect of hydroxytryptamine on canine intestinal motility during fasting. J Physiol 343:88–99

    Google Scholar 

  • Power AE (2004) Muscarinic cholinergic contribution tomemory consolidation: with attention to involvement of the basolateral amygdala. Curr Med Chem 11:987996

    Article  Google Scholar 

  • Rajathei DM, Preethi J, Singh HK, Rajan KE (2014) Molecular docking of bacosides with tryptophan hydroxylase: a model to understand the bacosides mechanism. Nat Prod Bioprospect 4:251–255

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rastogi M, Rudra P, Ojha PPC, Devi BP, Agrawal A, Dubey GP (2012) Prevention of age-associated neurodegeneration and promotion of healthy brain ageing in female Wistar rats by long term use of bacosides. Biogerontol 13:183–195

    Article  CAS  Google Scholar 

  • Ravaglia G, Forti P, Maioli F, Martelli M, Servadei L, Brunetti N, Dalmonte E, Bianchin M, Mariani E (2005) Incidence and etiology of dementia in a large elderly Italian population. Neurology 64:15251530

    Article  Google Scholar 

  • Sarin S, Gill KD (1999) Dichlorvos induced alterations in glucose homeostasis: possible implications on the state of neuronal function in rats. Mol and Cell Biochem 199:87–92

    Article  CAS  Google Scholar 

  • Sarin S, Gill KD (2000) Biochemical characterization of dichlorvos-induced delayed neurotoxicity in rat. IUBMB Life 49:125–130

    Article  CAS  PubMed  Google Scholar 

  • Singh HK, Dhawan BN (1997) Neuropsychopharmacological effects of the Ayurvedic nootropic Bacopa monniera Linn. (Brahmi). Indian J Pharmacol 29:S359–S365

    Google Scholar 

  • Taylor JT, Davis E, Dabisch P, Horsmon M, Matson K, Crouse C, Mioduszewski R (2008) Acute toxic effects of inhaled dichlorvos vapor on respiratory mechanics and blood cholinesterase activity in guinea pigs. Inhal Toxicol 20(5):465–472

    Article  CAS  PubMed  Google Scholar 

  • Udenfriend S, Weissbach H, Bogdanski DF (1957) Increase of tissue serotonin following administration of its precursor 5-hydroxytryptophan. J Biol Chem 224:803–810

    CAS  PubMed  Google Scholar 

  • Yadav P, Jadhav SE, Kumar V, Kaul KK, Pant SC, Flora SJS (2012) Protective efficacy of 2-PAMCl, atropine and curcumin against dichlorvos induced toxicity in rats. Interdiscip Toxicol 5(1):1–8

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

We thank Prof. Aditya Shastri, Vice Chancellor, Banasthali University and Department of Science and Technology (DST), India for providing the facilities for the present investigation.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Rajasthan, 304022, India

    Bharti Chaudhary & Renu Bist

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  1. Bharti Chaudhary
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  2. Renu Bist
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Correspondence to Renu Bist.

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Maintenance and treatment of animals were done in accordance with Committee for the Purpose of Control and Supervision of Experimentation on Animals (CPCSEA).

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Chaudhary, B., Bist, R. Protective manifestation of bacoside A and bromelain in terms of cholinesterases, gamma-amino butyric acid, serotonin level and stress proteins in the brain of dichlorvos-intoxicated mice. Cell Stress and Chaperones 22, 371–376 (2017). https://doi.org/10.1007/s12192-017-0773-1

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  • DOI: https://doi.org/10.1007/s12192-017-0773-1

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