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Chemopreventive effect of bacoside A on N-nitrosodiethylamine-induced hepatocarcinogenesis in rats

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Abstract

Purpose

Chemoprevention is an effective approach to control hepatocarcinogenesis. Bacoside A, the active constituent of Bacopa monniera Linn., is anticipated to play a role in chemoprevention of liver cancer.

Methods

In the present study, we investigated the chemopreventive effect of bacoside A against N-nitrosodiethylamine-induced hepatocarcinogenesis in an animal model.

Results

Administration of carcinogen showed a significant elevation in the levels of lipid peroxidation, serum tumor marker enzymes and liver injury marker enzymes with subsequent decrease in the levels of both hemolysate and liver antioxidant status. Bacoside A co-treatment maintained the N-nitrosodiethylamine-induced alterations at near normal level. Histopathological and electron microscopic study of the liver tissue also supports the above biochemical observations.

Conclusions

From our findings we conclude that bacoside A is effective to prevent DEN-induced hepatocellular carcinoma by quenching lipid peroxidation and enhancing antioxidant status through free radical scavenging mechanism and having potential of protecting endogenous enzymatic and non-enzymatic antioxidant activity.

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Acknowledgments

The authors wish to acknowledge Nivaran Herbal Pvt. Ltd, Chennai, India, for gifting crude bacoside A and Natural remedies Pvt. Ltd, Bangalore, India for gifting standard bacoside A to carry on the research work. The transmission electron microscopic studies from Department of Gastrointestinal sciences, CMC, Vellore is gratefully acknowledged. We are thankful to Dr. C. S. Vijayalakshmi, Professor, Kilpauk Medical College, Chennai for helping in histopathological studies.

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Authors and Affiliations

  1. Department of Zoology, Presidency College, Chennai, 600005, Tamil Nadu, India

    Panneerselvam Janani & Kanakarajan Sivakumari

  2. Department of Biochemistry, Bharathi Women’s College, Chennai, 600108, Tamil Nadu, India

    Arumugam Geetha

  3. Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, 600113, Tamil Nadu, India

    Baskaran Ravisankar & Chandrakesan Parthasarathy

  4. Division of Digestive Diseases and Nutrition, Department of Internal Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA

    Chandrakesan Parthasarathy

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  1. Panneerselvam Janani
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Correspondence to Kanakarajan Sivakumari.

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Janani, P., Sivakumari, K., Geetha, A. et al. Chemopreventive effect of bacoside A on N-nitrosodiethylamine-induced hepatocarcinogenesis in rats. J Cancer Res Clin Oncol 136, 759–770 (2010). https://doi.org/10.1007/s00432-009-0715-0

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