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Chaperonin containing T-complex polypeptide subunit eta is a potential marker of joint contracture: an experimental study in the rat

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Cell Stress and Chaperones Aims and scope

Abstract

Joint contracture is a fibroproliferative disorder that restricts joint mobility, resulting in tissue degeneration and deformity. However, the etiology of joint contracture is still unknown. Chaperonin containing T-complex polypeptide subunit eta (CCT-eta) is reported to increase in fibrotic diseases. The purpose of this study was to investigate whether CCT-eta is implicated in joint contracture and to determine the role of CCT-eta in the progression of joint contracture by analyzing a rat model. We immobilized the left knee joint of rat by internal fixation for 8 weeks. The non-immobilized right leg served as a control. The range of motion (ROM) of the knee was investigated. Fibroblasts were obtained from the posterior joint capsule of the joints. The outcome was followed by quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, fibroblast migration assay, and collagen assay. The effect of CCT-eta on the functions of fibroblasts was observed by utilizing a short inhibitory RNA (siRNA) targeting CCT-eta. The ROM of the immobilized joints was significantly limited compared to the contralateral joints (p < 0.05). Fibroblasts derived from the contractive joints showed higher mRNA and protein expressions of CCT-eta in parallel with alpha-smooth muscle actin (α-SMA) compared to the cells from the contralateral knees (p < 0.05). siRNA-mediated downregulation of CCT-eta inhibited the expressions of both CCT-eta and α-SMA. Moreover, the reduction of CCT-eta also significantly decreased fibroblast functions such as cell mobility and collagen synthesis (all p < 0.05). Our findings indicate that CCT-eta appears to be a potential marker of joint contracture disease.

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Acknowledgments

This work was financially supported by the Natural Science Foundation of Guangdong Province, China (S2013010016193).

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The authors declare that they have no competing interests.

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    Authors and Affiliations

    1. Department of Orthopedic Surgery, The Third Affiliated Hospital of Sun Yat-sen University, 510000, Guangzhou, China

      Ronghan He, Zhe Wang, Yunxiang Lu, Jianhua Ren & Kun Wang

    2. Division of Biomedical Cell Biology, Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK

      Junqi Huang

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    1. Ronghan He
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    2. Zhe Wang
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    Correspondence to Kun Wang.

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    Ronghan He and Zhe Wang contributed equally to this work.

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    He, R., Wang, Z., Lu, Y. et al. Chaperonin containing T-complex polypeptide subunit eta is a potential marker of joint contracture: an experimental study in the rat. Cell Stress and Chaperones 20, 959–966 (2015). https://doi.org/10.1007/s12192-015-0624-x

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