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Heat shock protein 70 is upregulated in the intestine of intrauterine growth retardation piglets

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Cell Stress and Chaperones Aims and scope

Abstract

The objective of this study is to investigate the expression and distribution of heat shock protein 70 (Hsp70) in the intestine of intrauterine growth retardation (IUGR) piglets. Samples from the duodenum, prejejunum, distal jejunum, ileum, and colon of IUGR and normal-body-weight (NBW) piglets were collected at birth. The results indicated that the body and intestine weight of IUGR piglets were significantly lower than NBW piglets. The villus height and villus/crypt ratio in jejunum and ileum of IUGR piglets were significantly reduced compared to NBW piglets. These results indicated that IUGR causes abnormal gastrointestinal morphologies and gastrointestinal dysfunction. The mRNA of hsp70 was increased in prejejunum (P < 0.05), distal jejunum (P < 0.05), and colon in IUGR piglets. However, the hsp70 mRNA in ileum of piglets with IUGR was decreased. Similar to hsp70 mRNA, the protein levels of Hsp70 in prejejunum (P < 0.05), distal jejunum, and colon (P < 0.05) in IUGR piglets were higher than those in NBW piglets. These results indicated that the expression of Hsp70 in the intestinal piglets was upregulated by IUGR, and different intestinal sites had different responses to stress. Meanwhile, the localization of Hsp70 in the epithelial cells of the whole villi and intestinal gland rather than in the lamina propria and myenteron suggested that Hsp70 has a cytoprotective role in epithelial cell function and structure.

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Abbreviations

IUGR:

Intrauterine growth retardation

Hsp:

Heat shock protein

NBW:

Normal body weight

NRC:

National Research Council

SD:

Standard deviation

OD:

Optical density

HSF:

Heat shock factor

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Acknowledgements

This work was supported by a grant from the National Natural Science Foundation of China (30771569). We would like to thank Yuanxiao Wang and Jianjun Wang for their assistance regarding sample collection. We also thank Caiyong Chen for critical discussions and reading of the manuscript.

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  1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China

    Xiang Zhong, Tian Wang, Xuhui Zhang & Wei Li

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  1. Xiang Zhong
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  2. Tian Wang
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  4. Wei Li
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Correspondence to Tian Wang.

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Zhong, X., Wang, T., Zhang, X. et al. Heat shock protein 70 is upregulated in the intestine of intrauterine growth retardation piglets. Cell Stress and Chaperones 15, 335–342 (2010). https://doi.org/10.1007/s12192-009-0148-3

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