Abstract
Plant-derived extra-cellular vesicles are a new food resource, rich in functional factors. However, the extraction rate of nanoparticles is low. Therefore, in this experiment, single-factor experiments were carried out using Chinese wild rice as the raw material, and the vesicle-like nanoparticles were successfully extracted from Chinese wild rice. The optimization of Chinese wild rice nanoparticle (WRNP) extraction process was conducted based on the response surface method. When the extraction time was 1 hour (h), and the ratio of material to liquid was 1:8.48 (w/v), the protein concentration of WRNPs was 10.28 ± 0.31 mg/mL after repeated extraction for five times. The obtained WRNPs were characterized, and its stability, antioxidant activity, and cytotoxicity assays were also evaluated. The particle size and zeta potential were 150.40 ± 3.11 nm and −8.95 ± 0.11 mV, respectively. The molecular weight of protein was mainly between 25 and 37 kDa, and the β-folds were the main content in the secondary structure of protein. The nanoparticles remained stable for 22 days at 4 °C, and nanoparticles could be digested by the human body. In addition, the antioxidant activity of WRNPs with a protein concentration of 1.6 mg/mL was not significantly different from that of vitamin C. Finally, nanoparticles have no significant toxic effect. The current study can provide a more perfect method for water frying and a reference for the development of natural antioxidant plant nanoparticles.
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Data Availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
References
Andreas F, Thomas H, Andreas S, Michael M (2018) Influence of process conditions during aqueous protein extraction upon yield from pre-pressed and cold-pressed rapeseed press cake. Ind Crops Prod 112:236–246. https://doi.org/10.1016/j.indcrop.2017.12.011
Bakkari MA, Moni SS, Alshammari A, Salawi A, Sultan MH, Madkhali OA, Alqahtani SS, Alam MF, Shaheen ES, Elmobark ME (2022) Design, characterization, and immune augmentation of docosahexaenoic acid nanovesicles as a potential delivery system for recombinant HBsAg protein. Vaccines 10(6):954. https://doi.org/10.3390/vaccines10060954
Boateng ID, Mustapha A, Daubert CR, Kuehnel L, Flint-Garcia S, Agliata J, Wan CX, Somavat P (2023) Novel two-pot microwave extraction of purple corn pericarp’s phenolics and evaluation of the polyphenol-rich extract’s product quality, bioactivities, and structural properties. Food Bioprocess Technol. https://doi.org/10.1007/s11947-023-03072-7
Cao M, Yan H, Han X, Weng L, Wei Q, Sun X, Lu W, Wei Q, Ye J, Cai X, Hu C, Yin X, Cao P (2019) Ginseng-derived nanoparticles alter macrophage polarization to inhibit melanoma growth. J Immuno Cancer 7(1):326. https://doi.org/10.1186/s40425-019-0817-4
Chen Y, Zou C, Mastalerz M, Hu S, Gasaway C, Tao X (2015) Applications of micro-Fourier transform infrared spectroscopy (FTIR) in the geological sciences--a review. Int J Mol Sci 16(12):30223–30250. https://doi.org/10.3390/ijms161226227
Chmiel T, Saputro IE, Kusznierewicz B, Bartoszek A (2017) The impact of cooking method on the phenolic composition, total antioxidant activity and starch digestibility of rice (Oryza sativa L). J Food Process Preserv 42(1):e13383.1–e13383.12. https://doi.org/10.1111/JFPP.13383
Cui H, Pan HW, Wang PH, Yang XD, Zhai WC, Dong Y, Zhou HL (2018a) Essential oils from Carex meyeriana Kunth: optimization of hydrodistillation extraction by response surface methodology and evaluation of its antioxidant and antimicrobial activities. Ind Crops Prod 124:669–676. https://doi.org/10.1016/j.indcrop.2018.08.041
Cui P, Lin S, Han W, Jiang P, Zhu B, Sun N (2019) The formation mechanism of a sea cucumber ovum derived heptapeptide-calcium nanocomposite and its digestion/absorption behavior. Food Funct 10(12):8240–8249. https://doi.org/10.1039/c9fo01335k
Cui P, Lin S, Jin Z, Zhu B, Song L, Sun N (2018b) In vitro digestion profile and calcium absorption studies of a sea cucumber ovum derived heptapeptide-calcium complex. Food Funct 9(9):4582–4592. https://doi.org/10.1039/c8fo00910d
Damiani E, Solorio JA, Doyle AP, Wallace HM (2019) How reliable are in vitro IC values? Values vary with cytotoxicity assays in human glioblastoma cells. Toxicol Lett 302:28–34. https://doi.org/10.1016/j.toxlet.2018.12.004
Elmowafy M, Shalaby K, Al-Sanea MM, Hendawy OM, Salama A, Ibrahim MF, Ghoneim MM (2021) Influence of stabilizer on the development of luteolin nanosuspension for cutaneous delivery: an in vitro and in vivo evaluation. Pharmaceutics 13(11):1812. https://doi.org/10.3390/pharmaceutics13111812
Gao WJ, Hou M, Chen XX, Wang P, Ren JG, Liu JX (2021) Analysis of the mechanism of Astragalus vesicle-like nanoparticles reducing Blood glucose in db/db Diabetic mice by regulating intestinal microflora. Chinese J Exp Pharmacol 27(14):111–118. https://doi.org/10.13422/j.cnki.syfjx.20211556
Gong P, Guo Y, Chen X, Cui D, Wang M, Yang W, Chen F (2022) Structural characteristics, antioxidant and hypoglycemic activities of polysaccharide from Siraitia grosvenorii. Molecules (Basel, Switzerland) 27(13):4192. https://doi.org/10.3390/molecules27134192
Granata G, Riccobene C, Napoli E, Geraci C (2022) Polymeric nanocapsules containing fennel essential oil: their preparation, physicochemical characterization, stability over time and in simulated gastrointestinal conditions. Pharmaceutics 14(4):873. https://doi.org/10.3390/pharmaceutics14040873
Han SF, Hong Z, Zhai CK (2012) Protective potentials of wild rice (Zizania latifolia (Grised) Turcz) against obesity and liptoxicity induced by a high-fat/cholesterol diet in rats. Food Chem Toxicol 50(7):2263–2269. https://doi.org/10.1016/j.fct.2012.04.039
Hou XD, Yan N, Du YM, Liang H, Zhang ZF, Yuan XL (2020) Consumption of wild rice (Zizania latifolia) prevents metabolic associated fatty liver disease through the modulation of the gut microbiota in mice model. Int J Mol Sci 21(15):5375. https://doi.org/10.3390/ijms21155375
Jain S, Anal AK (2018) Preparation of eggshell membrane protein hydrolysates and culled banana resistant starch-based emulsions and evaluation of their stability and behavior in simulated gastrointestinal fluids. Food Res Int (Ottawa, Ont.) 103:234–242. https://doi.org/10.1016/j.foodres.2017.10.042
Jiang Y, Li C, Nguyen X, Muzammil S, Towers E, Gabrielson J, Narhi L (2011) Qualification of FTIR spectroscopic method for protein secondary structural analysis. J Pharm Sci 100(11):4631–4641. https://doi.org/10.1002/jps.22686
Jong OG, Kooijmans SAA, Murphy DE, Jiang L, Evers MJW, Sluijter JPG, Vader P, Schiffelers RM (2019) Drug delivery with extracellular vesicles: from imagination to innovation. Accounts Chem Res 52(7):1761–1770. https://doi.org/10.1021/acs.accounts.9b00109
Kim SQ, Kim KH (2022) Emergence of edible plant-derived nanovesicles as functional food components and nanocarriers for therapeutics delivery: potentials in human health and disease. Cells 11(14):2232. https://doi.org/10.3390/cells11142232
Kirubakaran D, Selvam K, Prakash P, Manimegalai P, Shivakumar MS, SenthilNathan S (2023) Preparation and characterization of biogenic silver nanoparticles using Strobilanthes cordifolia (Vahl) J.R.I. Wood leaves and its biological applications. Biotechnol Appl Biochem 70(2):870–884. https://doi.org/10.1002/bab.2406
Kong D, Han R, Yuan M, Xi Q, Du Q, Li P, Yang Y, Applegate B, Wang J (2023) Ultrasound combined with slightly acidic electrolyzed water thawing of mutton: effects on physicochemical properties, oxidation and structure of myofibrillar protein. Ultrason Sonochem 93:106309. https://doi.org/10.1016/j.ultsonch.2023.106309
Li CM, Song Q, Yin XL, Song RL, Chen G (2022) Preparation, characterization, and in vitro anticancer activity evaluation of broccoli-derived extracellular vesicle-coated astaxanthin nanoparticles. Molecules (Basel, Switzerland) 27(12):3955. https://doi.org/10.3390/molecules27123955
Li G, Wang S, Zhu F (2016a) Physicochemical properties of quinoa starch. Carbohydr Polym 137:328–338. https://doi.org/10.1016/j.carbpol.2015.10.064
Li S, Wang X, Li W, Yuan G, Pan Y, Chen H (2016b) Preparation and characterization of a novel conformed bipolymer paclitaxel-nanoparticle using tea polysaccharides and zein. Carbohydr Polym 146:52–57. https://doi.org/10.1016/j.carbpol.2016.03.042
Li SM, He FJ, Qin LQ, Yang KY, Qiao HZ (2021) Preparation and properties of extracellular vesicle-like nanoparticles from medicinal and edible plants. Pharm J 56:2086–2092. https://doi.org/10.16438/j.0513-4870.2021-0554
Li T, Wang L, Chen Z, Zhang X, Zhu Z (2020) Functional properties and structural changes of rice proteins with anthocyanins complexation. Food Chem 331:127336. https://doi.org/10.1016/j.foodchem.2021.129278
Liu J, Liu Z, Pang Y, Zhou H (2022) The interaction between nanoparticles and immune system: application in the treatment of inflammatory diseases. J Nanobiotechnol 20(1):127. https://doi.org/10.1186/s12951-022-01343-7
Madalena D, Fernandes JM, Avelar Z, Gonçalves RFS, Ramos ÓL, Vicente AA, Pinheiro AC (2022) Emerging challenges in assessing bio-based nanosystem’ behaviour under in vitro digestion focused on food applications - a critical view and future perspectives. Food Res Int (Ottawa, Ont.) 157:111417. https://doi.org/10.1016/j.foodres.2022.111417
Moghadasian MH, Kaur R, Kostal K, Joshi AA, Molaei M, Le K, Fischer G, Bonomini F, Favero G, Rezzani R, Gregorchuk BSJ, Leung SV, Wuzinski M, Seo AI, Bay DC (2019) Anti-atherosclerotic properties of wild rice in low-density lipoprotein receptor knockout mice: the gut microbiome, cytokines, and metabolomics study. Nutrients 11(12):2894. https://doi.org/10.3390/nu11122894
Mu J, Zhuang X, Wang Q, Jiang H, Deng ZB, Wang B, Zhang L, Kakar S, Jun Y, Miller D, Zhang HG (2014) Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles. Mol Nutr Food Res 58(7):1561–1573. https://doi.org/10.1002/mnfr.201300729
Nuntarat B, Smriti S, Muhammad BS, Anil KA (2020) Influence of whey protein-xanthan gum stabilized emulsion on stability and in vitro digestibility of encapsulated astaxanthin. J Food Eng 272:109859. https://doi.org/10.1016/j.jfoodeng.2019.109859
Nwachukwu ID, Sarteshnizi RA, Udenigwe CC, Aluko REA (2021) Concise review of current in vitro chemical and cell-based antioxidant assay methods. Molecules (Basel, Switzerland) 26(16):4865. https://doi.org/10.3390/molecules26164865
Pal CBT, Jadeja GC (2019) Microwave-assisted deep eutectic solvent extraction of phenolic antioxidants from onion (Allium cepa L.) peel: a Box–Behnken design approach for optimization. J Food Sci Technology 56:4211–4223. https://doi.org/10.1007/s13197-019-03891-7
Parvez S, Karole A, Mudavath SL (2022) Fabrication, physicochemical characterization and in vitro anticancer activity of nerolidol encapsulated solid lipid nanoparticles in human colorectal cell line. Colloids Surf B-Biointerfaces 215:112520. https://doi.org/10.1016/j.colsurfb.2022.112520
Perut F, Roncuzzi L, Avnet S, Massa A, Zini N, Sabbadini S, Giampieri F, Mezzetti B, Baldini N (2021) Strawberry-derived exosome-like nanoparticles prevent oxidative stress in human mesenchymal stromal cells. Biomolecules 11(1):87. https://doi.org/10.3390/biom11010087
Pillai R, Sreelekshmi PB, Meera AP (2022) Curcumin nanoparticles as promising anticancer agent against human colorectal cancer cell lines. Electrochem Soc Trans 107(1). https://doi.org/10.1149/10701.19325ecst
Ping Y, Li Y, Lü S, Sun Y, Zhang W, Wu J, Liu T, Li Y (2022) A study of nanometre aggregates formation mechanism and antipyretic effect in Bai-Hu-Tang, an ancient Chinese herbal decoction. Biomed Pharmacother = Biomed Pharmacother 124:109826. https://doi.org/10.1016/j.biopha.2020.109826
Qian J, Wang Y, Zhuang H, Yan W, Zhang J, Luo J (2021) Plasma activated water-induced formation of compact chicken myofibrillar protein gel structures with intrinsically antibacterial activity. Food Chem 351:129278. https://doi.org/10.1016/j.foodchem.2021.129278
Regente M, Georgina CM, Maldonado AM, Pinedo M, Jorrín J, Laura DLC (2009) Vesicular fractions of sunflower apoplastic fluids are associated with potential exosome marker proteins. FEBS Lett 583(20):3363–3366. https://doi.org/10.1016/j.febslet.2009.09.041
Reis GCL, Dala-Paula BM, Tavano OL, Guidi LR, Godoy HT, Gloria MBA (2020) In vitro digestion of spermidine and amino acids in fresh and processed Agaricus bisporus mushroom. Food Res Int 137. https://doi.org/10.1016/j.foodres.2020.109616
Ren B, Chen C, Li C, Fu X, You L, Liu RH (2017) Optimization of microwave-assisted extraction of Sargassum thunbergii polysaccharides and its antioxidant and hypoglycemic activities. Carbohydr Polym 173:192–201. https://doi.org/10.1016/j.carbpol.2017.05.094
Tan ZL, Li JF, Luo HM, Liu YY, Jin Y (2022) Plant extracellular vesicles: a novel bioactive nanoparticle for tumor therapy. Front Pharmacol 13:1006299. https://doi.org/10.3389/fphar.2022.1006299
Tao X, Zhan L, Huang Y, Li P, Liu B, Chen P (2022) Preparation, characterization and evaluation of capsaicin-loaded indica rice starch nanoparticles. Food Chem 386:132692. https://doi.org/10.1016/j.foodchem.2022.132692
Thaiphanit S, Schleining G, Anprung P (2016) Effects of coconut (Cocos nucifera L.) protein hydrolysates obtained from enzymatic hydrolysis on the stability and rheological properties of oil-in-water emulsions. Food Hydrocoll 60:252–264. https://doi.org/10.1016/j.foodhyd.2016.03.035
Wang F, Yuan M, Shao C, Ji N, Zhang H, Li C (2023) Momordica charantia-derived extracellular vesicles provide antioxidant protection in ulcerative colitis. Molecules (Basel, Switzerland) 28(17):6182. https://doi.org/10.3390/molecules28176182
Wang LL, Cao ZZ, Li NN, Jia CH, Zhang BJ, Niu M, Zhao SM, Xiong SB, Fang Z (2020) Effect of cooking intensity on thermodynamics and digestive properties of rice. China J Grain Oils 35(08):8–14. https://doi.org/10.1002/jsfa.11058
Wei Y, Li C, Dai L, Zhang L, Liu J, Mao L, Yuan F, Gao Y (2020) The construction of resveratrol-loaded protein-polysaccharide-tea saponin complex nanoparticles for controlling physicochemical stability and in vitro digestion. Food Funct 11(11):9973–9983. https://doi.org/10.1039/d0fo01741h
Xiao J, Feng S, Wang X, Long K, Luo Y, Wang Y, Ma J, Tang Q, Jin L, Li X, Li M (2018) Identification of exosome-like nanoparticle-derived microRNAs from 11 edible fruits and vegetables. PeerJ 6:e5186. https://doi.org/10.7717/peerj.5186
Xu X, Dai Z, Zhang Z, Kou X, You X, Sun H, Guo H, Liu M, Zhu H (2021) Fabrication of oral nanovesicle in-situ gel based on Epigallocatechin gallate phospholipid complex: application in dental anti-caries. Eur J Pharmacol 897:173951. https://doi.org/10.1016/j.ejphar.2021.173951
Yan N, Du Y, Liu X, Chu C, Shi J, Zhang H, Liu Y, Zhang Z (2018) Morphological characteristics, nutrients, and bioactive compounds of Zizania latifolia, and health benefits of its seeds. Molecules (Basel, Switzerland) 23(7):1561. https://doi.org/10.3390/molecules23071561
Yang H, Li Q (2022) Optimization of extraction process and the antioxidant activity spectrum-effect relationship of Angelica dahurica. Biomed Chromatogr: BMC 36(4):e5322. https://doi.org/10.1002/bmc.5322
Yu X, Chu M, Chu C, Du Y, Shi J, Liu X, Liu Y, Zhang H, Zhang Z, Yan N (2020b) Wild rice (Zizania spp.): A review of its nutritional constituents, phytochemicals, antioxidant activities, and health-promoting effects. Food Chem 331:127293. https://doi.org/10.1016/j.foodchem.2020.127293
Yu XT, Qi QQ, Li YL, Li N, Xie YN, Ding AM, Shi J, Du YM (2022) Metabolomics and proteomics reveal the molecular basis of colour formation in the pericarp of Chinese wild rice (Zizania latifolia). Food Res Int 162:112082. https://doi.org/10.1016/j.foodres.2022.112082
Yu XT, Yang T, Qi QQ, Du YM, Liu YH, Zhang HB, Zhang HF, Yan N (2020a) Comparison of the contents of phenolic compounds including flavonoids and antioxidant activity of rice (Oryza sativa) and Chinese wild rice (Zizania latifolia). Food Chem 5(344):128600. https://doi.org/10.1016/j.foodchem.2020.128600
Zhai CK, Lu CM, Zhang XQ, Sun GJ, Lorenz KJ (2001) Comparative study on nutritional value of Chinese and North American wild rice. J Food Compos Anal 14(4):371–382. https://doi.org/10.1006/jfca.2000.0979
Zhang J, Zhu Y, Si J, Wu L (2022) Metabolites of medicine food homology-derived endophytic fungi and their activities. Curr Res Food Sci 5:1882–1896. https://doi.org/10.1016/j.crfs.2022.10.006
Zhu Y, Zhou Y, Tian T, Wang Z, Qi B, Zhang X, Liu J, Li Y, Jiang L, Wang Z (2019) In vitro simulated digestion and microstructure of peppermint oil nanoemulsion. J Oleo Sci 68(9):863–871. https://doi.org/10.5650/jos.ess19102
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This work was supported by the National Natural Science Foundation of China No. 31800284. This work was supported by the Joint Innovative, Scientific and Technological Research Program for Yangzhou City and Yangzhou University No. YZ2020267. This work was supported by the Research Project of Key Laboratory of Culture and Tourism from Jiangsu Province No. 203560133.
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Chunmei Li and Meng Yuan: investigation, methodology, data curation, software, formal analysis, writing the entire manuscript, and validation. Qin Hou: supervision, validation, and review and editing. Jiamin Su and Yu Zhou: review and editing and validation. Yansong Chen: data curation. Haifeng Zhang: conceptualization, funding acquisition, supervision, review, and editing.
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Li, C., Yuan, M., Hou, Q. et al. Extraction, Optimization, Characterization, and Antioxidant Activities of Chinese Wild Rice Nanoparticles. Food Anal. Methods 17, 33–46 (2024). https://doi.org/10.1007/s12161-023-02542-0
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DOI: https://doi.org/10.1007/s12161-023-02542-0