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Development and Validation of Rapid Colorimetric Detection of Nitrite Concentration in Meat Products on a Polydimethylsiloxane (PDMS) Microfluidic Device

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Abstract

Sodium nitrite is a common practice additive in meat products for enhancing color, flavor, and microbial quality; however, several studies link its post-procession to the formation of carcinogenic N-nitrosamines. The aim of this study was to fabricate and validate a portable, rapid, and accurate microfluidic polydimethylsiloxane (PDMS) analytical device to determine the nitrite concentration in meat products. The colorimetric determination of nitrite using PDMS is based on Griess reaction. The proposed PDMS method was validated and the uncertainty evaluated according to Eurachem guidelines. Results demonstrated that PDMS device’s detection and quantification limits for nitrite were 0.1 and 0.4 mg L−1, respectively. A complete correlation (R2 = 0.99) and RSD value was 1.3–2.1% (inter-day precision) and 1.5–2.4% (intra-day precision). The PDMS method is highly robust, accurate 98.8%, and uncertainty measurement 3.5%. No significant differences between PDMS device and spectrophotometry were found during the determination of nitrite in examined processed meat products. The overall analysis of the PDMS device could be completed within 10 min. The PDMS-based device has potential applications for rapid detection and routinely screen the nitrite concentration of meat products and guarantee food safety.

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Acknowledgements

M.K.M would like to thank the Polish Academy of Sciences (PAS) and the Academy of Scientific Research and Technology (ASRT) for the financial support during the fellowship (PAS-ASRT 2019). Many thanks to Prof. Piotr Garstecki and Dr. Ladislav Derzsi directors of the Laboratory of Microfluidics and Complex Fluids, Institute Physical Chemistry, Polish Academy of Sciences, for hosting a part of this research project.

Funding

Partial financial support was received from the Polish Academy of Sciences (PAS) and the Academy of Scientific Research and Technology (ASRT) during the fellowship (PAS-ASRT 2019).

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Authors and Affiliations

  1. Department of Food Technology, Faculty of Agriculture, Benha University, Qaluobia, 13736, Egypt

    Mohamed K. Morsy

  2. Department of Basic and Applied Sciences, Faculty of Engineering, Arab Academy of Science, Technology, and Maritime Transport, Cairo, Egypt

    Osama M. Morsy

  3. Department of Food Control, Animal Health Research Institute, Shebin El Koom Branch, Agriculture Research Center of Egypt, El-Menoufia, 13829, Egypt

    Elsayed M. Abd-Elaaty

  4. Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Benha University, Qaluobia, 13736, Egypt

    Rasha Elsabagh

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  1. Mohamed K. Morsy
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Correspondence to Mohamed K. Morsy.

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Mohamed Morsy declares that he has no conflict of interest. Osama Morsy declares that he has no conflict of interest. Elsayed Abd-Elaaty declares that he has no conflict of interest. Rasha Elsabagh declares that she has no conflict of interest.

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New method for analysis, Meat products, and Food safety

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Morsy, M.K., Morsy, O.M., Abd-Elaaty, E.M. et al. Development and Validation of Rapid Colorimetric Detection of Nitrite Concentration in Meat Products on a Polydimethylsiloxane (PDMS) Microfluidic Device. Food Anal. Methods 15, 552–564 (2022). https://doi.org/10.1007/s12161-021-02139-5

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