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A Visual and Sensitive Detection of Escherichia coli Based on Aptamer and Peroxidase-like Mimics of Copper-Metal Organic Framework Nanoparticles

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Abstract

In order to monitor and respond rapidly to outbreaks of food-borne pathogens, establishing rapid and sensitive detection methods is becoming increasingly urgent. In this work, through the synthesis of copper-based metal-organic framework nanoparticles (Cu-MOF NPs) and functionalized with aptamers, we developed a colorimetric protocol to detect Escherichia coli. In a typical experimental procedure, aptamer 1 was immobilized onto microplate to act as capture probes. Cu-MOF NPs were synthesized and functionalized with streptavidin and biotinylated aptamer 2 to form the signal probes. In the presence of E. coli, aptamers on both capture and signal probes bind with E. coli and form a sandwich-type complex. The Cu-MOF NPs enable to catalyze the colorless peroxidase substrate to yield a colorimetric output signal. This peroxidase-like mimics-based colorimetric aptasensor showed a rapid and sensitive quantification of E. coli in the concentration range of 16~1.6 × 106 cfu/mL with a limit of quantitation (LOQ) of 16 cfu/mL and limit of detection (LOD) of 2 cfu/mL. The application of the developed assay for colorimetric measuring of milk samples was evaluated with satisfactory results. The developed Cu-MOF NPs-assisted visual method can be expected as a powerful alternative and promising tool for foodborne pathogen detection and control.

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Funding

This work was partially supported by National Key Research and Development Program of China (2018YFC1602905), National Natural Science Fund of China (NSFC 31871721, 31772086), Project funded by China Postdoctoral Science Foundation (2017M610299, 2018T110443), Jiangsu Province Postdoctoral Science Foundation (1701097B), and Young Elite Scientists Sponsorship Program by CAST (2017QNRC001).

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Nuo Duan, Shijia Wu & Zhouping Wang

  2. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Nuo Duan, Shijia Wu, Ying Zou & Zhouping Wang

  3. School of Basic Science, Tianjin Agricultural University, Tianjin, 300392, China

    Wei Yang

  4. International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, 214122, China

    Shijia Wu & Zhouping Wang

  5. Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, China

    Shijia Wu & Zhouping Wang

Authors
  1. Nuo Duan
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  2. Wei Yang
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  3. Shijia Wu
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  4. Ying Zou
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  5. Zhouping Wang
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Correspondence to Shijia Wu or Zhouping Wang.

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Conflict of Interest

Nuo Duan declares that she has no conflict of interest. Wei Yang declares that she has no conflict of interest. Shijia Wu declares that he has no conflict of interest. Ying Zou declares that she has no conflict of interest. Zhouping Wang declares that he has no conflict of interest.

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Duan, N., Yang, W., Wu, S. et al. A Visual and Sensitive Detection of Escherichia coli Based on Aptamer and Peroxidase-like Mimics of Copper-Metal Organic Framework Nanoparticles. Food Anal. Methods 13, 1433–1441 (2020). https://doi.org/10.1007/s12161-020-01765-9

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  • DOI: https://doi.org/10.1007/s12161-020-01765-9

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