Abstract
QuEChERS combined with ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was developed to analyze multi-class mycotoxins including aflatoxins, ochratoxins, and Alternaria toxins in apples and tomatoes. Using apples and tomatoes as the research models, the method performance had been evaluated, showing great convenience, high sensitivity, and good reliability. The limit of detections was of 0.05–20 μg/L. The average recovery values were from 71.3 to 119.4%. With respect to precision, the repeatability was lower than 14.4%. With the optimum method, risk monitoring of mycotoxins in fresh and stored apples and tomatoes was executed, which indicated the risk of contamination of Alternaria toxins in apples which were preserved over a period of time.
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Amoli-Diva M, Taherimaslak Z, Allahyari M, Pourghazi K, Manafi MH (2015) Application of dispersive liquid-liquid microextraction coupled with vortex-assisted hydrophobic magnetic nanoparticles based solid-phase extraction for determination of aflatoxin M1 in milk samples by sensitive micelle enhanced spectrofluorimetry. Talanta 134:98–104. https://doi.org/10.1016/j.talanta.2014.11.007
Arcella D, Eskola M, Ruiz JAG (2016) Dietary exposure assessment to Alternaria toxins in the European population. EFSA J 14(12):4654, 32 pp. https://doi.org/10.2903/j.efsa.2016.4654
Chen H, Gao G, Liu P, Rong P, Xin L, Lu C (2016) Determination of 16 polycyclic aromatic hydrocarbons in tea by simultaneous dispersive solid-phase extraction and liquid–liquid extraction coupled with gas chromatography–tandem mass spectrometry. Food Analy Methods 9:2374–2384. https://doi.org/10.1007/s12161-016-0427-4
Cho H-D et al (2019) Comprehensive analysis of multi-class mycotoxins in twenty different species of functional and medicinal herbs using liquid chromatography–tandem mass spectrometry. Food Control 96:517–526. https://doi.org/10.1016/j.foodcont.2018.10.007
De Berardis S, De Paola EL, Montevecchi G, Garbini D, Masino F, Antonelli A, Melucci D (2018) Determination of four Alternaria alternata mycotoxins by QuEChERS approach coupled with liquid chromatography-tandem mass spectrometry in tomato-based and fruit-based products. Food Res Int 106:677–685. https://doi.org/10.1016/j.foodres.2018.01.032
Díaz-Zaragoza M, Carvajal-Moreno M, Méndez-Ramírez I, Chilpa-Galván NC, Avila-González E, Flores-Ortiz CM (2014) Aflatoxins, hydroxylated metabolites, and aflatoxicol from breast muscle of laying hens. Poult Sci 93:3152–3162. https://doi.org/10.3382/ps.2014-04240
Dong H, Xian Y, Xiao K, Wu Y, Zhu L, He J (2019) Development and comparison of single-step solid phase extraction and QuEChERS clean-up for the analysis of 7 mycotoxins in fruits and vegetables during storage by UHPLC-MS/MS. Food Chem 274:471–479. https://doi.org/10.1016/j.foodchem.2018.09.035
Gbashi S, Njobeh PB, De Saeger S, De Boevre M, Madala NE (2020) Development, chemometric-assisted optimization and in-house validation of a modified pressurized hot water extraction methodology for multi-mycotoxins in maize. Food Chem 307:125526. https://doi.org/10.1016/j.foodchem.2019.125526
Huang LC, Zheng N, Zheng BQ, Wen F, Cheng JB, Han RW, Xu XM, Li SL, Wang JQ (2014) Simultaneous determination of aflatoxin M 1 , ochratoxin A, zearalenone and α-zearalenol in milk by UHPLC–MS/MS. Food Chem 146:242–249. https://doi.org/10.1016/j.foodchem.2013.09.047
Kong WJ, Liu SY, Qiu F, Xiao XH, Yang MH (2013) Simultaneous multi-mycotoxin determination in nutmeg by ultrasound-assisted solid-liquid extraction and immunoaffinity column clean-up coupled with liquid chromatography and on-line post-column photochemical derivatization-fluorescence detection. Analyst 138:2729–2739. https://doi.org/10.1039/c3an00059a
Kruve A, Kunnapas A, Herodes K, Leito I (2008) Matrix effects in pesticide multi-residue analysis by liquid chromatography-mass spectrometry. J Chromatogr A 1187:58–66. https://doi.org/10.1016/j.chroma.2008.01.077
Leal T, Abrunhosa L, Domingues L, Venancio A, Oliveira C (2019) BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation. Food Chem 300:125204. https://doi.org/10.1016/j.foodchem.2019.125204
Myresiotis CK, Testempasis S, Vryzas Z, Karaoglanidis GS, Papadopoulou-Mourkidou E (2015) Determination of mycotoxins in pomegranate fruits and juices using a QuEChERS-based method. Food Chem 182:81–88. https://doi.org/10.1016/j.foodchem.2015.02.141
Niessen WM, Manini P, Andreoli R (2006) Matrix effects in quantitative pesticide analysis using liquid chromatography-mass spectrometry. Mass Spectrom Rev 25:881–899. https://doi.org/10.1002/mas.20097
Noser J, Schneider P, Rother M, Schmutz H (2011) Determination of six Alternaria toxins with UPLC-MS/MS and their occurrence in tomatoes and tomato products from the Swiss market. Mycotoxin Res 27:265–271. https://doi.org/10.1007/s12550-011-0103-x
Qiao X, Yin J, Yang Y, Zhang J, Shao B, Li H, Chen H (2018) Determination of Alternaria mycotoxins in fresh sweet cherries and cherry-based products: method validation and occurrence. J Agric Food Chem 66:11846–11853. https://doi.org/10.1021/acs.jafc.8b05065
Romera D, Mateo EM, Mateo-Castro R, Gomez JV, Gimeno-Adelantado JV, Jimenez M (2018) Determination of multiple mycotoxins in feedstuffs by combined use of UPLC-MS/MS and UPLC-QTOF-MS. Food Chem 267:140–148. https://doi.org/10.1016/j.foodchem.2017.11.040
Shang L, Bai X, Chen C, Liu L, Li M, Xia X, Wang Y (2019) Isolation and identification of a Bacillus megaterium strain with ochratoxin A removal ability and antifungal activity. Food Control 106:106743. https://doi.org/10.1016/j.foodcont.2019.106743
Sun J, Weixi L, Yan Z, Xuexu H, Li W, Bujun W (2016) QuEChERS purification combined with ultrahigh-performance liquid chromatography tandem mass spectrometry for simultaneous quantification of 25 mycotoxins in cereals. Toxins 8:375. https://doi.org/. https://doi.org/10.3390/toxins8120375
Trombete F, Barros A, Vieira M, Saldanha T, Venâncio A, Fraga M (2016) Simultaneous determination of deoxynivalenol, deoxynivalenol-3-glucoside and nivalenol in wheat grains by HPLC-PDA with immunoaffinity column cleanup. Food Anal Methods 9:2579–2586. https://doi.org/10.1007/s12161-016-0450-5
Wang M, Jiang N, Xian H, Wei D, Shi L, Feng X (2016) A single-step solid phase extraction for the simultaneous determination of 8 mycotoxins in fruits by ultra-high performance liquid chromatography tandem mass spectrometry. J Chromatogr A 1429:22–29. https://doi.org/10.1016/j.chroma.2015.12.004
Xing Y, Meng W, Sun W, Li D, Yu Z, Tong L, Zhao Y (2016) Simultaneous qualitative and quantitative analysis of 21 mycotoxins in Radix Paeoniae Alba by ultra-high performance liquid chromatography quadrupole linear ion trap mass spectrometry and QuEChERS for sample preparation. J Chromatogr B 1031:202–213. https://doi.org/10.1016/j.jchromb.2016.07.008
Zhao Y, Yuan YC, Bai XL, Liu YM, Wu GF, Yang FS, Liao X (2020) Multi-mycotoxins analysis in liquid milk by UHPLC-Q-Exactive HRMS after magnetic solid-phase extraction based on PEGylated multi-walled carbon nanotubes. Food Chem 305:125429. https://doi.org/10.1016/j.foodchem.2019.125429
Zhou J, Xu JJ, Cong JM, Cai ZX, Zhang JS, Wang JL, Ren YP (2018) Optimization for quick, easy, cheap, effective, rugged and safe extraction of mycotoxins and veterinary drugs by response surface methodology for application to egg and milk. J Chromatogr A 1532:20–29. https://doi.org/10.1016/j.chroma.2017.11.050
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This work was supported by The National Key Research and Development Program of China (2017YFC1601600).
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Yan Tang declares no conflict of interest. Lei Mu declares no conflict of interest. Jiaxing Cheng declares no conflict of interest. Zhenxia Du declares no conflict of interest. Youyou Yang declares no conflict of interest.
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Highlight
• A QuEChERS method coupled with UHPLC-MS/MS was developed for multi-class mycotoxin analysis.
• The proposed method is robust as well as sensitive for determination of eleven mycotoxins.
• The methodology is applicable to routinely monitor the eleven mycotoxins in apples and tomatoes.
• AME was detected in tomato samples, and ALT, ALT-I, AOH, and AME were detected in apple samples
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Tang, Y., Mu, L., Cheng, J. et al. Determination of Multi-Class Mycotoxins in Apples and Tomatoes by Combined Use of QuEChERS Method and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry. Food Anal. Methods 13, 1381–1390 (2020). https://doi.org/10.1007/s12161-020-01753-z
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DOI: https://doi.org/10.1007/s12161-020-01753-z