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Comparison of HPLC Separation of Phenylalanine Enantiomers on Different Types of Chiral Stationary Phases

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Abstract

HPLC separation of phenylalanine by using chiral stationary phases (CSPs) with chiral selectors β-cyclodextrin, isopropylcarbamate cyclofructan 6, teicoplanin, and ristocetin were studied. The effects of mobile phase composition and column temperature from 0 to 30 °C on the retention and resolution of enantiomers were investigated. β-cyclodextrin and isopropylcarbamate cyclofructan 6 CSPs showed chiral recognition in normal phase separation mode. The sufficient enantioseparations (resolution values 1.59 and 2.75) were reached using teicoplanin and ristocetin-based stationary phases at 23 °C in reversed-phase mode with mobile phases consist of acetonitrile and water at ratios 75/25 and 60/40 (v/v), respectively. Chiral HPLC system coupled with circular dichroism and polarimetry detector could be used to determine enantiomeric elution order. The optimized and validated HPLC-UV method with teicoplanin-based chiral stationary phase was applied for determination of phenylalanine in real samples. Both of the enantiomeric forms were detected in samples of dietary supplements and L-phenylalanine in samples of energy drinks. Obtained recoveries were higher than 82% with an RSD less than 10%. The HPLC method showed good linearity (correlation coefficients > 0.998) in concentration range 0.1–500 μg mL−1. The limit of detection was 0.1 μg mL−1 for both enantiomers.

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Acknowledgements

The authors thanks D.W. Armstrong for the donation of chiral columns.

Funding

This study was funded by the Slovak Research and Development Agency under the contract no. APVV-15-0355 and by project Excellent teams of young researchers at Slovak University of Technology in Bratislava.

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Authors and Affiliations

  1. Faculty of Chemical and Food Technology, Institute of Analytical Chemistry, Slovak University of Technology in Bratislava, Radlinského 9, 812 37, Bratislava, Slovak Republic

    Anna Lomenova & Katarína Hroboňová

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  1. Anna Lomenova
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  2. Katarína Hroboňová
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Correspondence to Katarína Hroboňová.

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Anna Lomenova declares that she has no conflict of interest. Katarína Hroboňová declares that she has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Informed consent was obtained from all individual participants included in the study.

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Lomenova, A., Hroboňová, K. Comparison of HPLC Separation of Phenylalanine Enantiomers on Different Types of Chiral Stationary Phases. Food Anal. Methods 11, 3314–3323 (2018). https://doi.org/10.1007/s12161-018-1308-9

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  • DOI: https://doi.org/10.1007/s12161-018-1308-9

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