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Simple and Rapid Method for the Simultaneous Determination of Cholesterol and Retinol in Meat Using Normal-Phase HPLC Technique

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Abstract

A direct saponification and normal-phase high-performance liquid chromatography (NP-HPLC) procedure was developed for simultaneous determination of cholesterol and retinol in meat. A normal-phase silica column fitted with diode array detection at 208 nm for cholesterol and fluorescence detection (λ-excitation 344 nm/λ-emission 472 nm) for retinol, with mobile phase consisting of 2% (v/v) 2-propanol in n-hexane was used. Cholesterol was eluted at 10 min and retinol at 12.66 min. High linearity (R 2 > 0.9996 for both compounds) in calibration range was obtained. The LOD and LOQ values show the high sensitivity of the developed methodology for simultaneous determination of cholesterol and retinol in meat. Recovery results obtained in this study (98.67–102.14% for cholesterol and 91.72–98.27% for retinol) were between AOAC recommendations to validated method and were comparable to most recent studies in precision and accuracy. In addition, the present method showed high repeatability and reproducibility. As a general conclusion, the results indicate that the direct saponification, extraction, and HPLC analysis is an adequate method for cholesterol and retinol analysis in meat samples.

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Authors and Affiliations

  1. Centro Tecnológico de la Carne de Galicia, Rúa Galicia N° 4, Parque Tecnológico de Galicia, 32900, San Cibrao das Viñas, Ourense, Spain

    Rubén Domínguez & José M. Lorenzo

  2. Nutrition and Food Science Area, Preventive Medicine and Public Health, Food Sciences, Toxicology and Forensic Medicine Department, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, s/n, 46100, Burjassot, València, Spain

    Francisco J. Barba

  3. Food Technology Area, Faculty of Science, University of Vigo, 32004, Ourense, Spain

    Juan A. Centeno

  4. Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000, Zagreb, Croatia

    Predrag Putnik

  5. Food Engineering Department, Middle East Technical University, 06800, Ankara, Turkey

    Hami Alpas

Authors
  1. Rubén Domínguez
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  2. Francisco J. Barba
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  3. Juan A. Centeno
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  4. Predrag Putnik
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  5. Hami Alpas
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  6. José M. Lorenzo
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Correspondence to José M. Lorenzo.

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Funding

The authors would like to thank Xunta de Galicia (grant number IN607B 2016/28).

Conflict of Interest

Rubén Domínguez declares that he has no conflict of interest. Francisco J. Barba declares that he has no conflict of interest. Juan A. Centeno declares that he has no conflict of interest. Predrag Putnik declares that he has no conflict of interest. Hami Alpas declares that he has no conflict of interest. José M. Lorenzo declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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(If not applicable to the study) Not applicable.

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Domínguez, R., Barba, F.J., Centeno, J.A. et al. Simple and Rapid Method for the Simultaneous Determination of Cholesterol and Retinol in Meat Using Normal-Phase HPLC Technique. Food Anal. Methods 11, 319–326 (2018). https://doi.org/10.1007/s12161-017-1001-4

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  • DOI: https://doi.org/10.1007/s12161-017-1001-4

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