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A Simple and Rapid Method for Determination of Patulin in Juice by High Performance Liquid Chromatography Tandem Mass Spectrometry

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Abstract

A simple and rapid method was developed for analysis of patulin in juice using ultra performance liquid chromatography coupled with tandem mass spectrometry. The samples were purified by an Oasis MAX 96-Well Plate. Chromatographic separation was performed on an ACQUITY UPLC® HSS T3 column with gradient elution using acetonitrile and water as mobile phase. Analytes was quantified by external calibration curves over the ranges of 0.50–50 μg/L, with correlation coefficients > 0.9986. The LOD and LOQ in juice sample were 0.30 and 1.0 μg/L, respectively. Recoveries of the method (spiked at levels of 1, 5, 25 and 50 μg/L) ranged from 91.6% to 95.7%, while intraday and interday relative standard deviations were 6.87–11.0% and 6.53–11.6%, respectively.

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Authors and Affiliations

  1. Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing, 100013, China

    Yi Yang, Yunjia Yang, Bing Shao & Jing Zhang

  2. School of Public Health, Capital Medical University, Beijing, 100069, China

    Yi Yang, Bing Shao & Jing Zhang

Authors
  1. Yi Yang
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  2. Yunjia Yang
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  3. Bing Shao
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  4. Jing Zhang
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Corresponding author

Correspondence to Jing Zhang.

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Funding

This study was supported by the Science Research Foundation of Ministry of Science and Technology of the People’s Republic of China (grant number 2015FY111400).

Conflict of Interest

Yi Yang declares that she has no conflict of interest. Yunjia Yang declares that he has no conflict of interest. Bing Shao declares that he has no conflict of interest. Jing Zhang declares that she has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Informed consent is not applicable for the nature of this study.

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Yang, Y., Yang, Y., Shao, B. et al. A Simple and Rapid Method for Determination of Patulin in Juice by High Performance Liquid Chromatography Tandem Mass Spectrometry. Food Anal. Methods 10, 2913–2918 (2017). https://doi.org/10.1007/s12161-017-0859-5

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  • DOI: https://doi.org/10.1007/s12161-017-0859-5

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