Food Analytical Methods

, Volume 10, Issue 10, pp 3239–3246 | Cite as

Simultaneous Determination of Ractopamine, Chloramphenicol, and Zeranols in Animal-Originated Foods by LC-MS/MS Analysis with Immunoaffinity Clean-up Column

  • Xue Sun
  • Qiang Tang
  • Xiaoli Du
  • Cunxian Xi
  • Bobin Tang
  • Guomin Wang
  • Hua ZhaoEmail author


A novel analytical method employing immunoaffinity column (IAC) clean-up coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of ractopamine, chloramphenicol, and zeranols (α-zearalanol, β-zearalanol, zearalanone, α-zearalenol, β-zearalenol, and zearalenone) in animal-originated foods. The sample was first digested by β-glucuronidase/sulfatase and then extracted with ethyl acetate-diethyl ether (9:1, v/v). The extracted solution was evaporated to dryness and then the residue was dissolved by 2 mL of 50% acetonitrile solution. After filtration, 1 mL filtrate was diluted to 10 mL with PBS. The reconstituted solution was cleaned up with immunoaffinity column and then analyzed by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). The established method was shown to be sensitive efficient and reliable as indicated by the linearity (r 2 ≥ 0.9994), precision (RSD ≤ 1.7%), average recovery (72.3–103.2%), and the limit of detection (0.05–0.10 μg/kg). The method can be used for determination of trace residues of ractopamine, chloramphenicol, and zeranols in animal-originated foods.


HPLC-MS/MS Immunoaffinity column Ractopamine Chloramphenicol Zeranols Animal-originated foods 


Compliance with Ethical Standards

Conflict of Interest

Xue Sun declares that she has no conflict of interest. Qiang Tang declares that he has no conflict of interest. Xiaoli Du declares that she has no conflict of interest. Cunxian Xi declares that he has no conflict of interest. Bobin Tang declares that he has no conflict of interest. Guomin Wang declares that he has no conflict of interest. Hua Zhao declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Xue Sun
    • 1
  • Qiang Tang
    • 1
  • Xiaoli Du
    • 2
  • Cunxian Xi
    • 3
  • Bobin Tang
    • 3
  • Guomin Wang
    • 3
  • Hua Zhao
    • 4
    Email author
  1. 1.People’s Hospital of the Tibet Autonomous RegionLhasaChina
  2. 2.Department of Pharmacy, Peking Union Medical College HospitalChinese Academy of Medical ScienceBeijingChina
  3. 3.Chongqing Entry-Exit Inspection and Quarantine BureauChongqing Engineering Technology Research Center of Import and Export Food SafetyChongqingChina
  4. 4.College of PharmacyChongqing Medical UniversityChongqingChina

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