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Food Analytical Methods

, Volume 10, Issue 10, pp 3239–3246 | Cite as

Simultaneous Determination of Ractopamine, Chloramphenicol, and Zeranols in Animal-Originated Foods by LC-MS/MS Analysis with Immunoaffinity Clean-up Column

  • Xue Sun
  • Qiang Tang
  • Xiaoli Du
  • Cunxian Xi
  • Bobin Tang
  • Guomin Wang
  • Hua ZhaoEmail author
Article

Abstract

A novel analytical method employing immunoaffinity column (IAC) clean-up coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of ractopamine, chloramphenicol, and zeranols (α-zearalanol, β-zearalanol, zearalanone, α-zearalenol, β-zearalenol, and zearalenone) in animal-originated foods. The sample was first digested by β-glucuronidase/sulfatase and then extracted with ethyl acetate-diethyl ether (9:1, v/v). The extracted solution was evaporated to dryness and then the residue was dissolved by 2 mL of 50% acetonitrile solution. After filtration, 1 mL filtrate was diluted to 10 mL with PBS. The reconstituted solution was cleaned up with immunoaffinity column and then analyzed by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). The established method was shown to be sensitive efficient and reliable as indicated by the linearity (r 2 ≥ 0.9994), precision (RSD ≤ 1.7%), average recovery (72.3–103.2%), and the limit of detection (0.05–0.10 μg/kg). The method can be used for determination of trace residues of ractopamine, chloramphenicol, and zeranols in animal-originated foods.

Keywords

HPLC-MS/MS Immunoaffinity column Ractopamine Chloramphenicol Zeranols Animal-originated foods 

Notes

Compliance with Ethical Standards

Conflict of Interest

Xue Sun declares that she has no conflict of interest. Qiang Tang declares that he has no conflict of interest. Xiaoli Du declares that she has no conflict of interest. Cunxian Xi declares that he has no conflict of interest. Bobin Tang declares that he has no conflict of interest. Guomin Wang declares that he has no conflict of interest. Hua Zhao declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable

References

  1. Akhtar MH, Danis C, Sauve A, Barry C (1995) Gas chromatographic determination of incurred chloramphenicol residues in eggs following optimal extraction. J Chromatogr A 696(1):123–130CrossRefGoogle Scholar
  2. Allen EH (1984) Review of chromatographic methods for chloramphenicol residues in milk, eggs and tissues from food-producing animals. J Assoc Off Anal Chem 68(5):990–999Google Scholar
  3. Ashwin HM, Stead SL, Taylor JC, Startin JR, Richmond SF, Homer V, Bigwood T, Sharman M (2005) Development and validation of screening and confirmatory methods for the detection of chloramphenicol and chloramphenicol glucuronide using SPR biosensor and liquid chromatography–tandem mass spectrometry. Anal Chim Acta 529(1):103–108CrossRefGoogle Scholar
  4. Beloglazova NV, Speranskaya ES, De Saeger S, Hens Z, Abe S, Goryacheva IY (2012) Quantum dot based rapid tests for zearalenone detection. Anal Bioanal Chem 403(10):3013–3024CrossRefGoogle Scholar
  5. Dickson LC, Costain R, McKenzie D, Fesser AC, Macneil JD (2009) Quantitative screening of stilbenes and zeranol and its related residues and natural precursors in veal liver by gas chromatography-mass spectrometry. J Agric Food Chem 57(15):6536–6542CrossRefGoogle Scholar
  6. Dusi G, Bozzoni E, Assini W, Tognoli N, Gasparini M, Ferretti E (2009) Confirmatory method for the determination of resorcylic acid lactones in urine sample using immunoaffinity cleanup and liquid chromatography-tandem mass spectrometry. Anal Chim Acta 637(1):47–54CrossRefGoogle Scholar
  7. He L, Su Y, Zeng Z, Liu Y, Huang X (2007) Determination of ractopamine and clenbuterol in feeds by gas chromatography-mass spectrometry. Anim Feed Sci Technol 132(3):316–323CrossRefGoogle Scholar
  8. Hutchison HE, Pinkerton PH (1962) Marrow depression due to chloramphenicol. Scott Med J 7(2):96–97CrossRefGoogle Scholar
  9. Marin DE, Taranu I, Burlacu R, Tudor DS (2010) Effects of zearalenone and its derivatives on the innate immune response of swine. Toxicon 56(6):956–963CrossRefGoogle Scholar
  10. Minervini F, Lacalandra GM, Filannino A, Nicassio M, Visconti A, Dell’Aquila ME (2010) Effects of in vitro exposure to natural levels of zearalenone and its derivatives on chromatin structure stability in equine spermatozoa. Theriogenology 73(3):392–403CrossRefGoogle Scholar
  11. Ministry of Agriculture (2002) No. 235. Bulletin of the Ministry of Agriculture of the People’s Republic of ChinaGoogle Scholar
  12. Nielen MWF, Van Engelen MC, Zuiderent R, Ramaker R (2007) Screening and confirmation criteria for hormone residue analysis using liquid chromatography accurate mass time-of-flight, Fourier transform ion cyclotron resonance and orbitrap mass spectrometry techniques. Anal Chim Acta 586(1):122–129CrossRefGoogle Scholar
  13. Pleadin J, Persi N, Milić D, Vahčić N (2012) Determination of residual ractopamine concentrations by enzyme immunoassay in treated pig’s tissues on days after withdrawal. Meat Sci 90(3):755–758CrossRefGoogle Scholar
  14. Qu CH, Li XL, Zhang L, Xi CX, Wang GM, Li NB, Luo HQ (2011) Simultaneous determination of cimaterol, salbutamol, terbutaline and ractopamine in feed by SPE coupled to UPLC. Chromatographia 73(3–4):243–249CrossRefGoogle Scholar
  15. Schaut A, De Saeger S, Sergent T, Schneider YJ, Larondelle Y, Pussemier L, Van Peteghem C (2008) Study of the gastrointestinal biotransformation of zearalenone in a Caco-2 cell culture system with liquid chromatographic methods. J Appl Toxicol 28(8):966–973CrossRefGoogle Scholar
  16. Spurlock ME, Cusumano JC, Ji SQ, Anderson DB, Smith CK, Hancock DL, Mills SE (1994) The effect of ractopamine on β-adrenoceptor density and affinity in porcine adipose and skeletal muscle tissue. J Anim Sci 72(1):75–82CrossRefGoogle Scholar
  17. Tsikas D (2010) Quantitative analysis of biomarkers, drugs and toxins in biological samples by immunoaffinity chromatography coupled to mass spectrometry or tandem mass spectrometry: a focused review of recent applications. J Chromatogr B 878(2):133–148CrossRefGoogle Scholar
  18. U.S. Food and Drug Administration (1999) Federal Food, Drug and Cosmetic Act (FFDCA), Section 512, 22 December 1999, FDA, Washington, DCGoogle Scholar
  19. Urraca JL, Marazuela MD, Moreno-Bondi MC (2004) Analysis for zearalenone and α-zearalenol in cereals and swine feed using accelerated solvent extraction and liquid chromatography with fluorescence detection. Anal Chim Acta 524(1):175–183CrossRefGoogle Scholar
  20. Wong S, Silva F, Acheson J, Plant G (2013) An old friend revisited: chloramphenicol optic neuropathy. JRSM short reports 4(3):20CrossRefGoogle Scholar
  21. You LN, Li XL, Xi CX, Tang BB, Wang GM, Zhang L, Yuan ZZ, Zhao H (2012) Simultaneous determination of residues of six zeranols in eggs by high performance liquid chromatography with immunoaffinity cleanup column. Chin J Chromatogr 30(10):1021–1025CrossRefGoogle Scholar
  22. Yuan ZZ, Zhang L, Tang BB, Xi CX, Wang GM, You LN, Zhao H (2013) Determination of 16 sulfonamide residues in animal-originated foods by high performance liquid chromatography with immunoaffinity column. J Instrum Anal 32(3):478–482Google Scholar
  23. Zhang MZ, Wang MZ, Chen ZL, Fang JH, Fang MM, Liu J, Yu XP (2009) Development of a colloidal gold based lateral flow immunoassay for the rapid simultaneous detection of clenbuterol and ractopamine in swine urine. Anal Bioanal Chem 395(8):2591CrossRefGoogle Scholar
  24. Zhang QJ, Peng T, Chen DD, Xie J, Wang X, Wang GM, Nie CM (2013) Determination of chloramphenicol residuces in aquatic products using immunoaffinity column cleanup and high performance liquid chromatography with ultraviolet detection. J AOAC Int 96(4):897–901CrossRefGoogle Scholar
  25. Zinedine A, Soriano JM, Molto JC, Manes J (2007) Review on the toxicity, occurrence, metabolism, detoxification, regulations and intake of zearalenone: an oestrogenic mycotoxin. Food Chem Toxicol 45(1):1–18CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Xue Sun
    • 1
  • Qiang Tang
    • 1
  • Xiaoli Du
    • 2
  • Cunxian Xi
    • 3
  • Bobin Tang
    • 3
  • Guomin Wang
    • 3
  • Hua Zhao
    • 4
    Email author
  1. 1.People’s Hospital of the Tibet Autonomous RegionLhasaChina
  2. 2.Department of Pharmacy, Peking Union Medical College HospitalChinese Academy of Medical ScienceBeijingChina
  3. 3.Chongqing Entry-Exit Inspection and Quarantine BureauChongqing Engineering Technology Research Center of Import and Export Food SafetyChongqingChina
  4. 4.College of PharmacyChongqing Medical UniversityChongqingChina

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