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Hollow Fiber Liquid Phase Microextraction Method Combined with High-Performance Liquid Chromatography for Simultaneous Separation and Determination of Ultra-Trace Amounts of Naproxen and Nabumetone in Cow Milk, Water, and Biological Samples

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Abstract

A simple and highly sensitive method based on hollow fiber liquid phase microextraction combined with high-performance liquid chromatography and fluorescence detection has been developed for simultaneous separation, preconcentration, and determination of naproxen and nabumetone from water, wastewater, milk, and biological samples. Parameters affecting the microextraction efficiency were evaluated and optimized. Under optimum conditions (extractant (14 μL of 1-undecanol), sample pH (3.0), extraction time (20 min), stirring rate (600 rpm), temperature (45 °C), potassium chloride concentration (4.0 %) and sample volume (9 mL)), the limits of detection based on (S/N = 3) were 1.3 ng L−1 for naproxen and 2.9 ng L−1 for nabumetone. The intra- and inter-assay relative standard deviations for naproxen and nabumetone were in the ranges of 3.2–6.1 % and 6.5–9.5 %, respectively. The calibration curves were linear in concentration ranges of 4.0–300.0 ng L−1 and 9.0–300.0 ng L−1 for naproxen and nabumetone, respectively, with good coefficient of determination (r 2 > 0.999). The method was successfully applied to the determination of naproxen and nabumetone in cow milk, water, wastewater, human plasma, and urine samples.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Yazd University, Yazd, 89195-741, Iran

    Mohammad Asadi, Shayessteh Dadfarnia & Ali Mohammad Haji Shabani

  2. Khorassan Research Institute for Food Science and Technology, Mashhad, Iran

    Bijan Abbasi

Authors
  1. Mohammad Asadi
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  2. Shayessteh Dadfarnia
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  3. Ali Mohammad Haji Shabani
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  4. Bijan Abbasi
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Correspondence to Shayessteh Dadfarnia.

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This study was not funded by any organization.

Conflict of Interest

Mohammad Asadi declares that he has no conflict of interest. Shayessteh Dadfarnia declares that she has no conflict of interest. Ali Mohammad Haji Shabani declares that he has no conflict of interest. Bijan Abbasi declares that he has no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.

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Asadi, M., Dadfarnia, S., Haji Shabani, A.M. et al. Hollow Fiber Liquid Phase Microextraction Method Combined with High-Performance Liquid Chromatography for Simultaneous Separation and Determination of Ultra-Trace Amounts of Naproxen and Nabumetone in Cow Milk, Water, and Biological Samples. Food Anal. Methods 9, 2762–2772 (2016). https://doi.org/10.1007/s12161-016-0449-y

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  • DOI: https://doi.org/10.1007/s12161-016-0449-y

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