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Volatile Metabolites of Goat Cheeses Determined by Ion Mobility Spectrometry. Potential Applications in Quality Control

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Abstract

Headspace sampling coupled with multi-capillary column-ion mobility spectrometry (HS-MCC-IMS) has been used for extraction and identification of some volatile metabolites from different types of goat cheese samples. The only manual operation carried out in this method was the introduction of samples into vials; the following steps (headspace generation, injection of the volatiles compounds into MCC and IMS detection) were fully automated. The analysis by MCC-IMS allowed rapid and simple differentiation of goat cheeses made with raw and pasteurized milk. MCC-IMS produced multidimensional ion mobility spectra with different retention times and intensity information different for some individual metabolites. A total of six metabolites were identified in goat cheeses including 1 alcohol (1-hexanol), 1 ketone (2-nonanone), 2 aldehydes (octanal, trans-2-heptanal) and 2 esters (ethyl butanoate and propyl butanoate). Furthermore, the evolution of one targeted metabolite (ethyl butanoate) was monitored through different ripening times (60, 150, 180, 210, 240, 270 days). These preliminary results showed that this volatile compound increased during cheese ageing according to biochemical and microbial dynamic of the matrix, and therefore ethyl butanoate could be a potential marker of ripening time in these kinds of cheese samples. Therefore, the method could be used for quality control purposes in goat cheese producers.

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References

  • Abeijón Mukdsi MC, Medina RB, Álvarez MF, González NS (2009) Ester synthesis by lactic acid bacteria isolated from goat’s and ewes milk. Food Chem 117:242–247

    Article  Google Scholar 

  • Attaie R (2009) Quantification of volatile compounds in goat milk Jack cheese using static headspace gas. J Dairy Sci 92:2435–2443

    Article  CAS  Google Scholar 

  • Barron LJ, Redondo Y, Flanagan CE, Pérez-Elortondo FJ, Albisu M, de Nájera AI, Renobales M, Fernández-García E (2005) Comparison of the volatile composition and sensory characteristics of Spanish PDO cheeses manufactured from ewes’ raw milk and animal rennet. Int Dairy J 15:371–382

    Article  CAS  Google Scholar 

  • Beuvier E, Buchin S (2004) Raw milk cheeses. In: Fox PF, McSweeney PLH, Cogan TM, Ginee TP (eds) Cheese chemistry, Phys Microbio. vol 1 3rd edn. Elsevier Academic Press pp 319–345

  • Borsdorf H, Mayer T, Zarejousheghani M, Eiceman GA (2011) Recent developments in ion mobility spectrometry. Appl Spectrosc Rev 46:472–521

    Article  Google Scholar 

  • Cadwallader KR, Singh TK (2009) Flavours and off-flavours in milk and dairy products. In: McSweeney PLH, Fox PF (eds) Advance dairy chemistry, vol III, 3rd edn. Springer. University College, Cork, p 209

    Google Scholar 

  • Cocker CJ, Crawford RA, Johnston H, Singh LK, Creamer T (2005) The classification of cheese variety and maturity on the basis of statistical analysis of proteolysis data—a review. Int Dairy J 15:631–643

    Article  Google Scholar 

  • Curioni PMG, Bosset JO (2002) Key odorants in various cheese types as determined by gas chromatography-olfactometry. Int Dairy J 12:959–984

    Article  CAS  Google Scholar 

  • d’Acampora Zellner B, Dugo P, Dugo G, Mondello L (2008) Gas chromatography–olfactometry in food flavour analysis. J Chromatogr A 1186:123–143

    Article  Google Scholar 

  • Delgado JF, González-Crespo J, Cava R, García-Parra J, Ramírez R (2011) Formation of the aroma of a raw goat milk cheese during maturation analysed by SPME-GC-MS. Food Chem 129:1156–1163

    Article  CAS  Google Scholar 

  • Fox PF, Wallace JM (1997) Formation of flavour compounds in cheese. Adv Appl Microbiol 45:17–85

    Article  CAS  Google Scholar 

  • Fox PF, McSweeny PLH, Cogan TM, Guinee TP (2004) Biochemistry of cheese ripening. In: Fox PF, McSweeney PLH, Cogan TM, Ginee TP (eds) Cheese: chemistry, physics and microbiology. vol 1 3rd edn. Elsevier Academic Press

  • Garde S, Ávila M, Fernández-García E, Medina M, Núñez M (2007) Volatile compounds and aroma of Hispánico cheese manufactured using lacticin 481-producing Lactococcus lactis subsp. lactis INIA 639 as an adjunct culture. Int Dairy J 17:717–726

    Article  CAS  Google Scholar 

  • Garrido-Delgado R, Arce L, Valcárcel M (2012) Multi-capillary column-ion mobility spectrometry: a potential screening system to differentiate virgin olive oils. Anal Bioanal Chem 402:489–498

    Article  CAS  Google Scholar 

  • Guillén MD, Abascal B (2012) Nature and distribution of the volatile components in the different regions of an artisanal ripened sheep cheese. J Dairy Res 79:102–109

    Article  Google Scholar 

  • Gursoy O, Somervuo P, Alatossava T (2009) Preliminary study of ion mobility based electronic nose MGD-1 for discrimination of hard cheeses. J Food Eng 92:202–207

    Article  Google Scholar 

  • Hayaloglu AA, Brechany EY (2007) Influence of milk pasteurization and scalding temperature on the volatile compounds of Malatya, a farmhouse. Halloumi-type cheese. Lait 87:39–57

    Article  CAS  Google Scholar 

  • Horne J, Carpino S, Tuminello L, Rapisarda T, Corallo L, Licitra G (2005) Differences in volatiles, and chemical, microbial and sensory characteristics between artisanal and industrial Piacentinu Ennese cheeses. Int Dairy J 15:605–617

    Article  CAS  Google Scholar 

  • Husson F, Krumow KN, Cases E, Coyot P, Bisakowski B, Kermasha S, Belin JM (2005) Influence of medium composition and structure on the biosynthesis of the natural flavour 1-octen-3-ol by Penicilliun camemberti. Proc Biochem 40:1395–1400

    Article  CAS  Google Scholar 

  • Lelles Nogueira MC, Lubachevsky G, Rankin SA (2005) A study of the volatile composition of Minas cheese. LWT 38:555–563

    Article  Google Scholar 

  • Leonhardt JW (1996) A new ppb gas analyzer by means of GC-IMS. J Radioanal Nucl Chem 206:133–139

    Article  Google Scholar 

  • Maarse H, Visscher CA (1989) Volatile compounds in food: qualitative and quantitative data. TNO-CIVO Food Analysis Institute

  • Marilley L, Casey MG (2004) Flavours of cheese products: metabolic pathways, analytical tools and identification of producing strains. Int J Food Microbiol 90:139–159

    Article  CAS  Google Scholar 

  • Massotti F, Battelli G, De Noni I (2012) The evolution of chemical and microbiological properties of fresh goat milk cheese during its shelf life. J Dairy Sci 95:4760–4767

    Article  Google Scholar 

  • Medina RB, Oliszewski R, Abeijón MC, Van Nieuwenhove CP, González SN (2011) Sheep and goat’s dairy products from South America: microbiota and its metabolic activity. Small Rumin Res 101:84–91

    Article  Google Scholar 

  • Moio L, Addeo F (1998) Grana Padano cheese aroma. J Dairy Res 65:317–333

    Article  CAS  Google Scholar 

  • Molimard P, Spinnler HE (1996) Compounds involved in the flavour of surface mold-ripened cheeses: origins and properties. J Dairy Sci 79:169–184

    Article  CAS  Google Scholar 

  • Montel MC, Buchinb S, Mallet A, Delbes-Pausa C, Dominique A, Vuitton DA, Desmasures N, Berthier F (2014) Traditional cheeses: rich and diverse microbiota with associated benefits. Int J Food Microbiol 177:136–154

    Article  Google Scholar 

  • Morales MT, Berry AJ, McIntyre PS, Aparicio R (1998) Tentative analysis of virgin olive oil aroma by supercritical fluid extraction-high-resolution gas chromatography mass spectrometry. J Chromatogr A 819:267–275

    Article  CAS  Google Scholar 

  • Muir DD, Banks JM, Hunder EA (1997) A comparison of the flavor and texture of the Cheddar cheese of factory or farmhouse origin. Int Dairy J 7:479–485

    Article  Google Scholar 

  • Mullet A, Escriche I, Rosello C, Tarrazo J (1999) Changes in the volatile fraction during ripening of Mahon cheese. Food Chem 65:219–225

    Article  Google Scholar 

  • Munning DJ, Nursten HE (1985) Flavour of milk and milk products in development in dairy chemistry. Springer pp 217–285

  • Palencia G, Ibargoitia ML, Fresno M, Sopelana P, Guillén MD (2014) Complexity and uniqueness of the aromatic profile of smoked and unsmoked Herreño cheese. Molecules 19:7937–7958

  • Panseri S, Giani I, Mentasti T, Bellagamba F, Caprino F, Moretti VM (2008) Determination of flavour compounds in a mountain cheese by headspace sorptive extraction-thermal desorption-capillary gas chromatography spectrometry. LWT Food Sci Technol 41:185–192

    Article  CAS  Google Scholar 

  • Rodríguez-Alonso P, Centeno JA, Garabal JI (2009) Comparison of the volatile profiles of Arzúa-Ulloa and Tetilla cheese manufactured from raw and pasteurized milk. Food Sci Technol 442:1722–1728

    Google Scholar 

  • Vautz W, Zimmermann D, Hartmann M, Baumbach J, Nolte J, Jung J (2006) Ion mobility spectrometry for food quality and safety. Food Addit Contam 23:1064–1073

    Article  CAS  Google Scholar 

  • Yuceer YK, Tuncel B, Guneser O, Engin B, Isleten M, Yasar K, Mendes M (2009) Characterization of aroma-active compounds, sensory properties, and proteolysis in Ezine cheese. J Dairy Sci 92:4146–4157

    Article  CAS  Google Scholar 

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Acknowledgments

The authors acknowledge gratefully the support given to Janneth Gallegos by the Secretaría Nacional de Educación Superior Ciencia Tecnología & Innovación of Ecuador (SENASCYT) through the grant to develop Ph.D in Cordoba University (Spain).

This work was funded by DGI, Spanish Ministry of Economy and Competitiveness, within the framework of Project CTQ2011-23790.

Conflict of Interest

Authors do not have a financial relationship with the organization that sponsored the research. Janneth Gallegos declares that she has no conflict of interest. Rocío Garrido-Delgado declares that she has no conflict of interest. Lourdes Arce declares that she has no conflict of interest. Luis M. Medina declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects

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Authors and Affiliations

  1. Department of Analytical Chemistry, University of Córdoba, Campus de Rabanales, 14071, Córdoba, Spain

    Rocío Garrido-Delgado & Lourdes Arce

  2. Food Science and Technology Department, University of Córdoba, Campus de Rabanales, 14071, Córdoba, Spain

    Janneth Gallegos & Luis M. Medina

  3. Escuela Superior Politécnica de Chimborazo, Riobamba, Ecuador

    Janneth Gallegos

Authors
  1. Janneth Gallegos
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  2. Rocío Garrido-Delgado
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  3. Lourdes Arce
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  4. Luis M. Medina
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Correspondence to Luis M. Medina.

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Gallegos, J., Garrido-Delgado, R., Arce, L. et al. Volatile Metabolites of Goat Cheeses Determined by Ion Mobility Spectrometry. Potential Applications in Quality Control. Food Anal. Methods 8, 1699–1709 (2015). https://doi.org/10.1007/s12161-014-0050-1

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  • DOI: https://doi.org/10.1007/s12161-014-0050-1

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