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Determination of Deoxynivalenol-3-Glucoside in Cereals by Hydrophilic Interaction Chromatography with Ultraviolet Detection

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Abstract

A new hydrophilic interaction liquid chromatography (HILIC) method was developed for the determination of deoxynivalenol-3-glucoside (D3G) in cereals. D3G was extracted with water and cleaned with an immunoaffinity (IA) column, followed by chromatographic separation on a Syncronis HILIC column with acetonitrile-water (90:10, v/v) as the mobile phase, and detected at 220 nm by UV detection. The calibration curve was found to be linear in the range of 0.1–2.0 μg/ml. Limit of detection and limit of quantification were 8 and 25 μg/kg, respectively. The method was applied to the determination of D3G in 14 cereal samples, which were analyzed as well as by LC-MS/MS. The results indicate that the method can be considered as an alternative to LC-MS/MS and could be adopted by nonprofessional analytical labs.

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Acknowledgments

This work was financially supported by Joint Innovation Fund of Industry-University Research Institute of Jiangsu Province, China (Project no. BY2012208).

Conflict of Interest

Zhiming Geng has received a research grant from Science and Technology Department of Jiangsu Province, China. Dan yang has received a research grant from Jiangsu Academy of Agricultural Sciences, China. Miaoping Zhou has received a research grant from Science and Technology Department of Jiangsu Province, China. Pingping Zhang has received a research grant from the National Natural Science Foundation of China (NSFC). Daoying Wang has received a research grant from the NSFC. Fang Liu has received a research grant from the NSFC. Yongzhi Zhu declares that he has no conflict of interest. Muhan Zhang declares that she has no conflict of interest. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People’s Republic of China

    Zhiming Geng, Daoying Wang, Fang Liu, Yongzhi Zhu & Muhan Zhang

  2. Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People’s Republic of China

    Dan Yang, Miaoping Zhou & Pingping Zhang

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  1. Zhiming Geng
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  2. Dan Yang
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  3. Miaoping Zhou
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  7. Yongzhi Zhu
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Correspondence to Zhiming Geng.

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Geng, Z., Yang, D., Zhou, M. et al. Determination of Deoxynivalenol-3-Glucoside in Cereals by Hydrophilic Interaction Chromatography with Ultraviolet Detection. Food Anal. Methods 7, 1139–1146 (2014). https://doi.org/10.1007/s12161-013-9726-1

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