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Pyrogallol: an Alternative Trapping Agent in Proanthocyanidins Analysis

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Abstract

The use of pyrogallol as trapping reagent in proanthocyanidin (PA) analysis was investigated. PAs are antioxidants and disease-preventing agents, the efficiency of which depends on their composition and size. Different nucleophilic compounds are usually used for acid-catalyzed depolymerization of proanthocyanidins: benzyl mercaptan, phloroglucinol, and cysteamine. Each nucleophile has different characteristics and typical reaction temperatures that ranges from 50 to 90 °C. Pyrogallol is more reactive than these compounds, so it was possible to apply a minor reaction temperature that was finally reduced from 50 to 30 °C, compared to phloroglucinol, but maintaining a significant yield. The results, compared to those obtained using phloroglucinol as trapping nucleophile, indicated that pyrogallol is an effective and interesting reagent for this analysis.

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Acknowledgments

Dr. Matteo Bordiga was a recipient of a fellowship from Italian Politiche Agricole Alimentari e Forestali Ministry (Food-Link project) and Dr. Monica Locatelli from Cariplo Foundation (Nutrial Network 2010 project; cod. 2009-2961).

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Authors and Affiliations

  1. Dipartimento di Scienze del Farmaco, Universita’ degli Studi del Piemonte Orientale “A. Avogadro”, Largo Donegani 2, 28100, Novara, Italy

    Matteo Bordiga, Jean Daniel Coïsson, Monica Locatelli, Marco Arlorio & Fabiano Travaglia

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  1. Matteo Bordiga
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  2. Jean Daniel Coïsson
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  3. Monica Locatelli
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  4. Marco Arlorio
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  5. Fabiano Travaglia
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Correspondence to Fabiano Travaglia.

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Bordiga, M., Coïsson, J.D., Locatelli, M. et al. Pyrogallol: an Alternative Trapping Agent in Proanthocyanidins Analysis. Food Anal. Methods 6, 148–156 (2013). https://doi.org/10.1007/s12161-012-9427-1

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  • DOI: https://doi.org/10.1007/s12161-012-9427-1

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