Abstract
A cloud-point extraction (CPE) method using Tergitol TMN-6 (TMN-6) non-ionic surfactant was developed for the extraction and preconcentration of propyl gallate (PG), tertiary butyl hydroquinone (TBHQ), butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT) in edible oils. The optimum conditions of CPE were 1.5 % (v/v) Tergitol TMN-6, 1 % (w/v) NaCl, ultrasound-assist 15 min at 49 KHz, 20 min equilibrated at 45 °C, and centrifugation for 10 min at 3,000 rpm. The surfactant-rich phase was then analyzed by reversed-phase high-performance liquid chromatography (HPLC) with ultraviolet detection at 280 nm, under gradient separation, using methanol and 1.5 % (v/v) acetic acid. Under the study conditions, four synthetic phenolic antioxidants (SPAs) were successfully separated within 24 min. Limits of detection in the studied edible oils were in the range of 1.6 to 9.0 ng mL−1. The high recoveries of the spiked edible oils were obtained in the range 90–98 %. The CPE method has been shown to be a potentially useful methodology for the preconcentration of the target analytes, with a preconcentration factor of 25. This method was compared with cloud point extraction (using Triton X-114) and liquid–liquid extraction (using methanol).
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References
Aparicio A, San Andres MP, Vera S (2000) Hrc-J High Resolut Chromatogr 23:324–328
Aranda PR, Gil RA, Moyano S, De Vito IE, Martinez LD (2008) Talanta 75:307–311
Bai DS, Li JL, Chen SB, Chen BH (2001) Environ Sci Technol 35:3936–3940
Bianchi L, Colivicchi MA, Della Corte L, Valoti M, Sgaragli GP, Bechi P (1997) J Chromatogr B: Biomed Sci Appl 694:359–365
C.o.F.R. Food and Drug Administration (2001) In: C.o.F.R. Food and Drug Administration (ed) Washington, DC
Capitan-Vallvey LF, Valencia MC, Nicolas EA (2004) Anal Chim Acta 503:179–186
Carabias-Martinez R, Rodriguez-Gonzalo E, Moreno-Cordero B, Perez-Pavon JL, Garcia-Pinto C, Laespada EF (2000) J Chromatogr A 902:251–265
Chen M, Xia Q, Liu M, Yang Y (2011) J Food Sci 76:C98–C103
Clayson DB, Iverson F, Nera E, Lok E, Rogers C, Rodrigues C, Page D, Karpinski K (1986) Food Chem Toxicol: An Int J Publ British Ind Biol Res Assoc 24:1171–1182
Delgado B, Pino V, Ayala JH, Gonzalez V, Afonso AM (2004) Anal Chim Acta 518:165–172
Delgado-Zamarreno MM, Gonzalez-Maza I, Sanchez-Perez A, Carabias Martinez R (2007) Food Chem 100:1722–1727
C. Directive (1995) In: C. Directive (ed) Brussels, pp 1
Fernandez AE, Ferrera ZS, Rodriguez JJS (1998) Anal Chim Acta 358:145–155
Goryacheva IY, Shtykov SN, Loginov AS, Panteleeva IV (2005) Anal Bioanal Chem 382:1413–1418
Guo L, Xie M-Y, Yan A-P, Wan Y-Q, Wu Y-M (2006) Anal Bioanal Chem 386:1881–1887
Ito N, Fukushima S, Tamano S, Hirose M, Hagiwara A (1986) J Nat Cancer Inst 77:1261–1265
Jia GF, Lv CG, Zhu WT, Qiu J, Wang XQ, Zhou ZQ (2008a) J Hazard Mater 159:300–305
Jia G, Lv C, Zhu W, Qiu J, Wang X, Zhou Z (2008b) J Hazard Mater 159:300–305
Karovicova J, Simko P (2000) J Chromatogr A 882:271–281
Kolachi NF, Kazi TG, Khan S, Wadhwa SK, Baig JA, Afridi HI, Shah AQ, Shah F (2011) Food ChemToxicol 49:2548–2556
Liu W, Zhao W-j, Chen J-b, Yang M-m (2007) Anal Chim Acta 605:41–45
Luconi MO, Silva MF, Olsina RA, Fernandez LP (2000) Talanta 51:123–129
Luque M, Rios A, Valcarcel M (1999) Anal Chim Acta 395:217–223
Merino F, Rubio S, Perez-Bendito D (2002) J Chromatogr A 962:1–8
Miura J, Ishii H, Watanabe H (1976) Bunseki Kagaku 25:808–809
Moran G, Harimanow L, Finlayson R, Hook J (2000) Abstr Pap Am Chem Soc 219:U88–U88
Mustafina A, Elistratova J, Burilov A, Knyazeva I, Zairov R, Amirov R, Solovieva S, Konovalov A (2006) Talanta 68:863–868
Ohashi A, Ogiwara M, Ikeda R, Okada H, Ohashi K (2004) Anal Sci 20:1353–1357
Ohashi A, Ito H, Kanai C, Imura H, Ohashi K (2005) Talanta 65:525–530
Ohashi A, Hashimoto T, Imura H, Ohashi K (2007) Talanta 73:893–898
Perrin C, Meyer L (2002) Food Chem 77:93–100
Pinho O, Ferreira I, Oliveira M, Ferreira MA (2000) Food Chem 68:353–357
Purkait MK, Vijay SS, DasGupta S, De S (2004) Dyes Pigments 63:151–159
Purkait MK, Banerjee S, Mewara S, DasGupta S, De S (2005) Water Res 39:3885–3890
Purkait MK, DasGupta S, De S (2006) J Hazard Mater 137:827–835
Purkait MK, DasGupta S, De S (2009) Desalination 244:130–138
Riber J, de la Fuente C, Vazquez MD, Tascon ML, Batanero PS (2000) Talanta 52:241–252
Rodil R, Benito Quintana J, Basaglia G, Pietrogrande MC, Cela R (2010) J Chromatogr A 1217:6428–6435
Ruiz MA, Garcia-Moreno E, Barbas C, Pingarron JM (1999) Electroanalysis 11:470–474
Saad B, Sing YY, Nawi MA, Hashim N, Ali ASM, Saleh MI, Sulaiman SF, Talib KM, Ahmad K (2007) Food Chem 105:389–394
Shah F, Kazi TG, Afridi HI, Naeemullah, Arain MB, Baig JA (2011) J Hazard Mater 192:1132–1139
Shen JC, Shao XG (2006) Anal Chim Acta 561:83–87
Sicilia D, Rubio S, Perez-Bendito D, Maniasso N, Zagatto EAG (1999) Anal Chim Acta 392:29–38
Sikalos TI, Paleologos EK (2005) Anal Chem 77:2544–2549
Sin DWM, Wong YC, Mak CY, Sze ST, Yao WY (2006) J Food Compos Anal 19:784–791
Sirimanne SR, Barr JR, Patterson DG Jr, Ma L (1996) Anal Chem 68:1556–1560
Sun ZM, Liang P, Ding Q, Cao J (2006) Anal Sci 22:911–913
Tang AN, Jiang DQ, Yan XP (2004) Anal Chim Acta 507:199–204
Tombesi NB, Freije H (2002) J Chromatogr A 963:179–183
Ulusoy HI, Akcay M, Ulusoy S, Gurkan R (2011) Anal Chim Acta 703:137–144
Wu D, Wu Q, Bian R, Wang Y (2007) Sep Sci Technol 42:2693–2704
Wuilloud GM, de Wuilloud JCA, Wuilloud RG, Silva MF, Olsina RA, Martinez LD (2002) Talanta 58:619–627
Yang TJ, Tsai FJ, Chen CY, Yang TCC, Lee MR (2010) Anal Chim Acta 668:188–194
Zhang W, Duan C, Wang M (2011a) Food Chem 126:779–785
Zhang WJ, Duan CM, Wang ML (2011b) Food Chem 126:779–785
Zhao W-j, Sun X-k, Deng X-n, Huang L, Yang M-m, Zhou Z-m (2011) Food Chem 127:683–688
Ziyatdinova GK, Gainetdinova AA, Budnikov GK (2010) J Anal Chem 65:929–934
Acknowledgement
This work was supported by the Analysis Test Research Center of Kunming University of Science and Technology, Yunnan Province, China. Innovation Fund for Small and Medium Technology Based Firms (No. 10C26215305131). Thank you to Daniel Paddon for the assistance with editing this article.
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Chen, M., Hu, X., Tai, Z. et al. Determination of Four Synthetic Phenolic Antioxidants in Edible Oils by High-Performance Liquid Chromatography with Cloud Point Extraction Using Tergitol TMN-6. Food Anal. Methods 6, 28–35 (2013). https://doi.org/10.1007/s12161-012-9413-7
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DOI: https://doi.org/10.1007/s12161-012-9413-7