Abstract
A simple and green high performance liquid chromatographic method was developed to determine the acesulfame K, saccharin, aspartame, and caffeine content in soft drinks without the use of toxic organic solvents. Separation was carried out on a C18 column using ethanol–phosphate buffer as the mobile phase at 0.8 mL/min. Optimum separation was achieved in 15 min. Acesulfame K, saccharin, aspartame, and caffeine were detected at 225, 217, 217, and 280 nm, respectively. The calibration curves were linear (correlation coefficient ≥0.999) in the selected range of each analyte. The limit of detection was 0.024 mg/L for acesulfam K, 0.011 mg/L for saccharin, 0.024 mg/L for caffeine, and 0.014 mg/L for aspartame. The relative standard deviations in inter and intra-laboratory precision studies varied from 0.44% to 1.61%. Recoveries of the analytes from spiked diet cola samples were between 98.40% and 100.25%. The validated method was found to be accurate and precise in the working calibration range. The developed analysis method was applied to 56 soft drink samples and was statistically compared with a conventional method by using test material. In conclusion, the developed HPLC analysis method provided more accurate values than the conventional analysis method.
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References
Anastas PT (1999) Crit Rev Anal Chem 29:167–175
AOAC (2002) obtained from web site: http://wwwaoacorg/Official_Methods/slv_guidelinespdf
Armenta S, Garrigues S, De La Guardia M (2008) Trac-Trend Anal Chem 27:497–511
Barbas C, Garcia A, Saavedra L, Castro M (2000) J Chromatogr A 870:97–103
Demiralay EC, Özkan G, Seydim ZG (2006) Chromatographia 63:91–96
Dossi N, Toniolo R, Susmel S, Pizzariello A, Bontempelli G (2006) Chromatographia 63:557–562
Guillarme D, Heinisch S, Rocca JL (2004) J Chromatogr A 1052:39–51
Heinisch S, Rocca JL (2009) J Chromatogr A 1216:642–658
Jastrzębska A (2011) J Food Compos Anal 24:1049–1056
Kirchhoff MM (2005) Resour Conserv Recycl 44:237–243
Koel M, Kaljurand M (2006) Pure Appl Chem 78:1993–2002
Kromidas S (2004) Practical problem solving in HPLC. WILEY-VCH Verlag GmbH, Germany, 186 p
Llorenc M, Capella-Peiro ME, Gil-Agusti M, Esteve-Romero J (2003) J Chromatogr A 984:223–231
López Ortíz CM, Prats Moya MS, Berenguer Navarro V (2006) J Food Compos Anal 19:141–149
Mahnaz K, Mannan H, Ali MR, Mohammad RO, Mohammad RSA, Ghazaleh M (2011) Food Anal Methods. doi:10.1007/s12161-011-9259-4
Nishitani E, Sagesaka YM (2004) J Food Compos Anal 17:675–685
Pandey S (2006) Anal Chim Acta 556:38–45
Peng L, Song X, Shi X, Li J, Ye C (2008) J Food Compos Anal 21:559–563
Prodolliet J, Bruelhart M (1993a) J AOAC Int 76:268–274
Prodolliet J, Bruelhart M (1993b) J AOAC Int 76:275–282
Ruiz-Angel MJ, Caballero RD, Simó-Alfonso EF, García-Alvarez-Coque MC (2002) J Chromatogr A 947:31–45
Smith RM (2008) J Chromatogr A 1184:441–455
Taverniers I, De Loose M, Bockstaele EV (2004) Trac-Trend Anal Chem 23:535–552
Vidottia EC, Costaa WF, Oliveira CC (2006) Talanta 68:516–521
Warner JC, Cannon AS, Dye KM (2004) Environ Impact Asses 24:775–799
Welch CJ, Wu N, Biba M, Hartman R, Brkovic T, Gong X, Helmy R, Schafer W, Cuff J, Pirzada Z, Zhou L (2010) Trac-Trend Anal Chem 29:667–680
Xie X, Wang X, Zhao L (2011) Food Anal Methods 4:203–211
Xin Y, Jing W, Xu DC, Qiu JW, Ying M, Cui J (2011) Food Anal Methods 4:186–195
Yang Y, Strickland Z, Kapalavavi B, Marple R, Gamsky C (2011) Talanta 84:169–174
Zygler A, Wasik A, Namiesnik J (2009) Trac-Trend Anal Chem 28:1082–1110
Acknowledgments
This research was supported The Scientific Research Projects Coordination Unit of Akdeniz University. Grateful thanks are due to Dr. A. Goncu Suru for her help in the inter-day accuracy and precision of the method. The author thanks specially the firm SIFA (Burdur, Turkey) for supplying the matrix blank used in this paper.
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Sik, B. Development and Validation of a Green High Performance Liquid Chromatographic Method for the Determination of Some Artificial Sweeteners and Caffeine in Soft Drinks. Food Anal. Methods 5, 1443–1452 (2012). https://doi.org/10.1007/s12161-012-9385-7
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DOI: https://doi.org/10.1007/s12161-012-9385-7