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Establishment and Validation of a Time-Resolved Fluoroimmunoassay for Chlorpromazine

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Abstract

The objective of this study is to establish a highly sensitive, time-resolved fluoroimmunoassay (TRFIA) for the rapid detection of chlorpromazine (CPZ). The antigen CPZ-OVA was used to coat a microtiter plate to compete with free chlorpromazine for binding to the anti-chlorpromazine monoclonal antibody. Europium (Eu3+)-labeled goat anti-mouse antibody was used as the tracer. The labeling rate of Eu3+-labeled goat anti-mouse antibody was 10.5 Eu3+/antibody. The average intra- and inter-batch coefficients of variation were 4.0% and 6.4%, respectively, and the average recovery rate was 90.27%. The sensitivity was 0.2 μg/L over a measurable range of 0.2–50 μg/L. The ED20, ED50, and ED80 were 1.17, 4.85, and 17.12 μg/L, respectively. CPZ-TRFIA is one of the most sensitive methods for CPZ detection. This analytical method demonstrates good stability and a wide measurable range, demonstrating its potential for practical applications.

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Acknowledgments

This work was financially supported by the Hi-Tech Program, Ministry of Science and Technology, China.

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Authors and Affiliations

  1. Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu, China, 214063

    Biao Huang, Jue Zhang, Yi Zhang, Kai Zhang & Minhao Xie

  2. School of Medicine and Pharmaceutics, Southern Yangtze University, Wuxi, Jiangsu, China, 214122

    Lili Zhao, Junming Sun & Jian Jin

Authors
  1. Biao Huang
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  2. Lili Zhao
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  3. Junming Sun
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  4. Jue Zhang
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  5. Yi Zhang
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  6. Kai Zhang
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  7. Jian Jin
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  8. Minhao Xie
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Correspondence to Minhao Xie.

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Huang, B., Zhao, L., Sun, J. et al. Establishment and Validation of a Time-Resolved Fluoroimmunoassay for Chlorpromazine. Food Anal. Methods 5, 625–630 (2012). https://doi.org/10.1007/s12161-011-9286-1

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  • DOI: https://doi.org/10.1007/s12161-011-9286-1

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