Abstract
A new liquid chromatographic method with an ion trap detector for the determination of chloramphenicol residues in animal feed is described. The developed method consists of an organic extraction and a two-step cleanup. For the cleanup, solid phase and liquid–liquid extractions were applied to minimize the ion suppression effects. One precursor and three product ions were monitored obtaining 5.5 identification points, which provided the method with a very high selectivity/specificity and gave a great confidence to the results. The described method was fully validated according to the requirements of the 2002/657/EC European Decision and the estimated decision limit and detection capability were 6 and 8 μg Kg−1, respectively.
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12161-011-9261-x/MediaObjects/12161_2011_9261_Fig1_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12161-011-9261-x/MediaObjects/12161_2011_9261_Fig2_HTML.gif)
Similar content being viewed by others
References
Antignac JP, De Wasch K, Monteau F, De Brabander HF, Andre F, Le Bizec B (2005) Anal Chim Acta 529:129
Commission Decision 2003/181/EC of 13 March 2003, Off J Eur Commun. L71, 17, (2003)
Commission Regulation 1430/94 of 22 June 1994, Off J Eur Commun. L156 6, (1994)
Council Directive 96/23/EC of 29 April 1996, Off J Eur Commun. L125, 10, (1996)
CRL guidance document (December 2007), http://www.rivm.nl/bibliotheek/digitaaldepot/crlguidancepaper2007.pdf
Forti AF, Campana G, Simonella A, Multari M, Scortichini G (2005) Anal Chim Acta 529:257–263
Gantverg A, Shishani I, Hoffman M (2003) Anal Chim Acta 483:125–135
Hormazábal V, Yndestad M (2001) J Liq Chromatogr Relat Technol 24:2477–2486
Impens S, Reybroeck W, Vercammen J, Courtheyn D, Ooghe S, DeWasch K, Smedts W, DeBrabander H (2003) Anal Chim Acta 483:153–163
Mottier P, Parisod V, Gremaud E, Guy PA, Stadler RH (2003) J Chrom A 994:75–84
Nicolich RS, Werneck-Barroso E, Sipoli Marques MA (2006) Anal Chim Acta 565:97–102
Ramos M, Munoz P, Aranda A, Rodriguez I, Diaz R, Blanca J (2003) J Chrom B 791:31–38
Thorsen H, Ronning K, Einarsen TN (2006) Asp. J Chrom A 1118:226–233
Acknowledgment
The authors would like to thank C. Navarro, C. Gargallo, X. Saez, M. Lamas and A. Miro for their assistance in experimental work.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Moragues, F., Igualada, C. & León, N. Validation of the Determination of Chloramphenicol Residues in Animal Feed by Liquid Chromatography with an Ion Trap Detector Based on European Decision 2002/657/EC. Food Anal. Methods 5, 416–421 (2012). https://doi.org/10.1007/s12161-011-9261-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12161-011-9261-x