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Xylooligosaccharides from Pretreated Rice Bran Produced by Immobilized Xylanase

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Abstract

Xylooligosaccharides (XOS) are potential prebiotic ingredients for food industries, mainly obtained after xylan hydrolysis by endoxylanases. Enzyme immobilization offers opportunities for recovery and reuse, while also enhancing its physical and chemical characteristics, such as stability and catalytic efficiency. This work aimed to immobilize the SM2 xylanase derived from the xynA gene from Orpinomyces sp. PC-2 and to evaluate its potential for XOS production. For this, SM2 xylanase was immobilized using the cross-linking methodology. The free and immobilized enzymes were characterized regarding the effect of pH, temperature, and thermostability. The cross-linked enzyme aggregate was evaluated for reuse and storage conditions and used for xylooligosaccharide production. Both free and immobilized SM2 xylanase showed maximal activity at 60 °C. The immobilized enzyme was more active at acidic and neutral conditions, and the free enzyme showed greater activity at basic conditions. The half-life of the free and immobilized xylanase was 30 and 216 h, respectively. In reuse tests, enzymatic activity increased with each cycle, and there was no statistical difference in the activity of SM2 xylanase aggregate stored at 4 and 25 °C. After saccharification, xylobiose (0.895 g/L), xylotriose (0.489 g/L), and xylohexose (0.809 g/L) were detected. As a result, immobilization enhanced thermostability, shifted the pH of maximum activity to 5, facilitated reuse, and eliminated the need for refrigerated packaging. Finally, the xylooligosaccharides produced by the SM2 xylanase are known for their prebiotic role, providing potential application of the immobilized enzyme in the food industry.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil

    Letícia Persilva Fernandes, Rafaela Zandonade Ventorim, Micael Garcia de Oliveira, Lucas Filipe Almeida, Valéria Monteze Guimarães & Gabriela Piccolo Maitan-Alfenas

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  1. Letícia Persilva Fernandes
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  2. Rafaela Zandonade Ventorim
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  3. Micael Garcia de Oliveira
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  4. Lucas Filipe Almeida
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  5. Valéria Monteze Guimarães
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  6. Gabriela Piccolo Maitan-Alfenas
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Contributions

LPF participated in the study design and conducted analysis; RZV participated in the analysis of experimental data and manuscript preparation; MGO participated in the execution of biochemical characterization; LFA participated in the XOS production evaluation; VMG contributed to the study design and analysis of experimental data; and GPMA coordinated study design, analysis, and manuscript preparation.

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Correspondence to Gabriela Piccolo Maitan-Alfenas.

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Fernandes, L.P., Ventorim, R.Z., de Oliveira, M.G. et al. Xylooligosaccharides from Pretreated Rice Bran Produced by Immobilized Xylanase. Bioenerg. Res. (2024). https://doi.org/10.1007/s12155-024-10770-3

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