Abstract
High quantity of potato wastage from the process and cold storage facilities in India poses serious disposal issues and loss of carbonaceous starches. Waste potato biomass has the potential estimated to be 6.3–7.2 MMT of fermentable sugar equivalent per annum. This study evaluates ethanol and yeast lipid production feasibility using the non-marketable waste potato. For obtaining optimum sugar, the dilute acid hydrolysis was first optimized by one variable at a time (OVAT) approach, and an artificial neural network was formulated to model the hydrolysis process. The optimized variables considered were reaction time, temperature, and acid concentration. The optimum glucose concentration of 55.82 g/L was utilized as a carbon source for ethanol and yeast lipid production by Saccharomyces cerevisiae and Rhodotorula mucilaginosa IIPL32. Ethanol and lipid concentrations of 33.74 and 2.6 g/L were obtained, respectively. The carbon balance of the two processes showed that the flow of net carbon concerning the feedstock was more towards the waste streams. The finding, therefore, proposes that with proper supply chain management, this fermentable carbon can be destined as a co-feed in any existing distillery, or even a separate decentralized system can be envisaged. This study thus investigates the stepwise carbon mapping from the feedstock to the product leading to a cradle to gate carbon flow assessment to understand the net carbon sustainability.
Similar content being viewed by others
Abbreviations
- WPB:
-
Waste potato biomass
- FNN:
-
Feed forward neural network
- DCW:
-
Dry cell weight
- RMIIPL32:
-
Rhodotorula mucilaginosa IIPL32
References
Carmona-Cabello M, Garcia IL, Leiva-Candia D, Dorado MP (2018) Valorization of food waste based on its composition through the concept of biorefinery. Curr Opin Green Sustain Chem 14:67–79
Smith P, Beaumont L, Bernacchi CJ, Byrne M, Cheung W, Conant RT, Cotrufo F, Feng X, Janssens I, Jones H and Kirschbaum MU (2021) Essential outcomes for COP26. Glob Chang Biol
Khalid I, Ullah S, Umar IS (2022) The problem of solid waste: origins, composition, disposal, recycling, and reusing. Int J Adv Sci Comput Appl 1(1):27–40
Di-Maria F, Lovat E, Caniato M (2018) Waste management in developed and developing countries: The case study of Umbria (Italy) and the west bank (Palestine). Detritus 3:171–180
Tomiyama JM, Takagi D, Kantar MB (2020) The effect of acute and chronic food shortage on human population equilibrium in a subsistence setting. Agric Food Secur 9(1):1–12
Kiran EU, Trzcinski AP, Ng WJ, Liu Y (2014) Bioconversion of food waste to energy: a review. Fuel 134:389–399
Robertson GH, Wong DW, Lee CC, Wagschal K, Smith MR, Orts WJ (2006) Native or raw starch digestion: a key step in energy-efficient biorefining of grain. J Agric Food Chem 54(2):353–365
The Indian Express (2020) About 16% of potatoes produced in-country dumped as waste. [online] Available at: <https://indianexpress.com/article/cities/ahmedabad/about-16-of-potatoes-produced-in-country-dumped-as-waste-6241880/> [Accessed 11 November 2021]
News B and News I (2019) Agricultural wastage is India’s problem no 1 — here is why — times of India. [online] The Times of India. Available at: <https://timesofindia.indiatimes.com/business/india-business/agricultural-wastage-is-indias-problem-no-1-here-is-why/articleshow/70974705.cms> [Accessed 3 November 2021]
Komlaga GA, Oduro I, Ellis WO, Dziedzoave NT (2021) Effect of harvest age of cassava roots and sweet potato tubers on alcohol yield. Afr J Food Sci 15(4):169–176
Zhu F, Cai YZ, Ke J, Corke H (2010) Compositions of phenolic compounds, amino acids, and reducing sugars in commercial potato varieties and their effects on acrylamide formation. J Sci Food Agric 90(13):2254–2262
Kim K, Hamdy MK (1985) Acid hydrolysis of sweet potato for ethanol production. Biotechnol Bioeng 27(3):316–320
Li D, Yang N, Zhou X, Jin Y, Guo L, Xie Z, Jin Z, Xu X (2017) Characterization of acid hydrolysis of granular potato starch under induced electric field. Food Hydrocolloids 71:198–206
Luo C, Brink DL, Blanch HW (2002) Identification of potential fermentation inhibitors in conversion of hybrid poplar hydrolyzate to ethanol. Biomass and Bioenergy 22(2):125–138
Sirohi R, Pandey JP, Singh A, Sindhu R, Lohani UC, Goel R, Kumar A (2020) Acid hydrolysis of damaged wheat grains: modeling the formation of reducing sugars by a neural network approach. Ind Crop Prod 149:112351
Górnicki K, Kaleta A, Trajer J (2019) Modelling of dried apple rehydration indices using ANN. Int Agrophys 33(3):285–296
Dhanya MS (2022) Perspectives of agro-waste biorefineries for sustainable biofuels. In: Nandabalan YK, Garg VK, Labhsetwar NK, Singh A (eds) Zero waste biorefinery. Energy, Environment, and Sustainability. Springer, Singapore. https://doi.org/10.1007/978-981-16-8682-5_8
Awasthi MK, Sindhu R, Sirohi R, Kumar V, Ahluwalia V, Binod P, Juneja A, Kumar D, Yan B, Sarsaiya S, Zhang Z (2022) Agricultural waste biorefinery development towards circular bioeconomy. Renew Sustain Energy Rev 158:112122
Bandhu S, Dasgupta D, Akhter J, Kanaujia P, Suman SK, Agrawal D, Kaul S, Adhikari DK, Ghosh D (2014) Statistical design and optimization of single-cell oil production from sugarcane bagasse hydrolysate by an oleaginous yeast Rhodotorula sp. IIP-33 using response surface methodology. SpringerPlus 3(1):1–11
Bansal N, Dasgupta D, Hazra S, Bhaskar T, Ray A, Ghosh D (2020) Effect of utilization of crude glycerol as a substrate on the fatty acid composition of an oleaginous yeast Rhodotorula mucilagenosa IIPL32: assessment of nutritional indices. Bioresour Technol 309:123330
Khot M, Raut G, Ghosh D, Alarcón-Vivero M, Contreras D, Ravikumar A (2020) Lipid recovery from oleaginous yeasts: perspectives and challenges for industrial applications. Fuel 259:116292
Pirt SJ, Whelan WJ (1951) The determination of starch by acid hydrolysis. J Sci Food Agric 2(5):224–228
Amirkhani H, Yunus R, Rashid U, Salleh SF, Radhiah AD, Syam S (2015) Low-temperature dilute acid hydrolysis of oil palm frond. Chem Eng Commun 202(9):1235–1244
Silva DD, Arruda PV, Dussán KJ, Felipe MG (2014) Adaptation of scheffersomyces stipitis cells as a strategy to the improvement of ethanol production from sugarcane bagasse hemicellulosic hydrolysate. Chem Eng 38
Niju S, Swathika M, Balajii M (2020) Pretreatment of lignocellulosic sugarcane leaves and tops for bioethanol production. In Lignocellulosic biomass to liquid biofuels (pp. 301–324). Academic Press. https://doi.org/10.1016/B978-0-12-815936-1.00010-1
Królikowska K, Pietrzyk S, Łabanowska M, Kurdziel M, Pająk P (2021) The influence of acid hydrolysis on physicochemical properties of starch-oleic acid mixtures and generation of radicals. Food Hydrocoll 118:106780
Aguado D, Ribes J, Montoya T, Ferrer J, Seco A (2009) A methodology for sequencing batch reactor identification with artificial neural networks: a case study. Comput Chem Eng 33(2):465–472
Yang S, Tian Y, He C, Zhang X, Tan KC, Jin Y (2021) A gradient-guided evolutionary approach to training deep neural networks. IEEE Transactions on Neural Networks and Learning Systems.
Sindhu R, Kuttiraja M, Binod P, Janu KU, Sukumaran RK, Pandey A (2011) Dilute acid pretreatment and enzymatic saccharification of sugarcane tops for bioethanol production. Biores Technol 102(23):10915–10921
Izmirlioglu G, Demirci A (2012) Ethanol production from waste potato mash by using Saccharomyces cerevisiae. Appl Sci 2(4):738–753
Lim Y, Jang Y, Kim K (2013) Production of a high concentration of ethanol from potato tuber by high gravity fermentation. Food Sci Biotechnol 22(2):441–448
Kot AM, Pobiega K, Piwowarek K, Kieliszek M, Błażejak S, Gniewosz M, Lipińska E (2020) Biotechnological methods of management and utilization of potato industry waste—a review. Potato Research 63(3):431–447
Khot M, Ghosh D (2017) Lipids of Rhodotorula mucilaginosa IIPL32 with biodiesel potential: oil yield, fatty acid profile, fuel properties. J Basic Microbiol 57(4):345–352
Sharma T, Sailwal M, Dasgupta D, Hazra S, Bhaskar T, Ghosh D (2021) Effect of lignocellulosic biomass inhibitors on oleaginous yeast cultivation in the multistage fermentation system. Bioresour Technol Rep 15:100791
Li M, Liu GL, Chi Z, Chi ZM (2010) Single-cell oil production from hydrolysate of cassava starch by marine-derived yeast Rhodotorula mucilaginosa TJY15a. Biomass Bioenerg 34(1):101–107
Martins LC, Palma M, Angelov A, Nevoigt E, Liebl W, Sá-Correia I (2021) Complete utilization of the major carbon sources present in sugar beet pulp hydrolysates by the oleaginous red yeasts Rhodotorula toruloides and R. mucilaginosa. J Fungi 7(3):215
Sheppard AJ (1992) Lipid manual: methodology suitable for fatty acid-cholesterol analysis. Wm. C. Brown Publishers.
Funding
This work was supported by CSIR AN&B FBR grants MLP-1167 and MLP-1168. Author AB has received research support from UGC-JRF.
Author information
Authors and Affiliations
Contributions
Ayan Banerjee: methodology, formal analysis, investigation, writing — original draft; Megha Sailwal: formal analysis, investigation; Mohammad Hafeez: methodology, investigation; Arijit Jana: review and editing; Jyoti Porwal: formal analysis; Thallada Bhaskar: resources, writing — review and editing, supervision; Debashish Ghosh: formal analysis, editing, supervision, project administration.
Corresponding author
Ethics declarations
Competing Interests
The authors declare no competing interests.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Banerjee, A., Sailwal, M., Hafeez, M. et al. Dilute Acid Hydrolysis and Bioconversion of Waste Potato to Ethanol and Yeast Lipid for Evaluating Carbon Flow in Waste Biorefinery. Bioenerg. Res. 16, 203–212 (2023). https://doi.org/10.1007/s12155-022-10433-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12155-022-10433-1