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Simultaneous Saccharification and Ethanol Fermentation of Corn Stover at High Temperature and High Solids Loading by a Thermotolerant Strain Saccharomyces cerevisiae DQ1

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Abstract

The thermotolerant strain Saccharomyces cerevisiae DQ1 was applied to the simultaneous saccharification and fermentation (SSF) at high temperature and high solids loading of the dilute acid-pretreated corn stover in the present study. The SSF using S. cerevisiae DQ1 was operated at 30 % solids loading of the pretreated corn stover with three-step SSF mode and achieved up to ethanol titer of 48 g/L and yield of 65.6 %. S. cerevisiae DQ1 showed strong thermotolerance in both the regular one-step SSF and the three-step SSF with changing temperature in each step. The three-step SSF at 40°C using S. cerevisiae DQ1 tolerated the greater cellulase dosage and solids loading of the pretreated corn stover and resulted in increased ethanol production. The present study provided a practical potential for the future SSF of lignocellulose feedstock at high temperature to reach high ethanol titer.

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Acknowledgments

This research was supported by National Basic Research Program of China (2011CB707406), Natural Science Foundation of China (20976051), and China Postdoctoral Science Foundation (2011M500742).

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  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China

    Deqiang Chu, Jian Zhang & Jie Bao

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  1. Deqiang Chu
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  2. Jian Zhang
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  3. Jie Bao
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Correspondence to Jie Bao.

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Chu, D., Zhang, J. & Bao, J. Simultaneous Saccharification and Ethanol Fermentation of Corn Stover at High Temperature and High Solids Loading by a Thermotolerant Strain Saccharomyces cerevisiae DQ1. Bioenerg. Res. 5, 1020–1026 (2012). https://doi.org/10.1007/s12155-012-9219-x

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  • DOI: https://doi.org/10.1007/s12155-012-9219-x

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