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IR-MALDI ion mobility spectrometry: physical source characterization and application as HPLC detector

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International Journal for Ion Mobility Spectrometry

Abstract

Infrared matrix-assisted laser dispersion and ionization (IR-MALDI) in combination with ion mobility (IM) spectrometry enables the direct analysis of biomolecules in aqueous solution. The release of ions directly from an aqueous solution is based on a phase explosion, induced by the absorption of an IR laser pulse, which disperses the liquid as vapor, nano- and micro-droplets. The ionization process is characterized initially by a broad spatial distribution of the ions, which is a result of complex fluid dynamics and desolvation kinetics. These processes have a profound effect on the shape and width of the peaks in the IM spectra. In this work, the transport of ions by the phase explosion-induced shockwave could be studied independently from the transport by the electric field. The shockwave-induced mean velocities of the ions at different time scales were determined through IM spectrometry and shadowgraphy. The results show a deceleration of the ions from 118 m∙s−1 at a distance of 400 μm from the liquid surface to 7.1 m∙s−1 at a distance of 10 mm, which is caused by a pile-up effect. Furthermore, the desolvation kinetics were investigated and a first-order desolvation constant of 325 ± 50 s−1 was obtained. In the second part, the IR-MALDI-IM spectrometer is used as an HPLC detector for the two-dimensional separation of a pesticide mixture.

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Acknowledgments

For financial support the authors would like to thank the German Excellence Initiative (DFG – Deutsche Forschungsgemeinschaft), the School of Analytical Sciences Adlershof (SALSA) and the Bundesanstalt für Materialforschung und—prüfung (BAM). Furthermore, we would also like to thank Ales Charvat (IOM Leipzig) for providing the microbeam nozzle mount and accompanying our first steps in IR-MALDI, as well as Sandro Andreotti (FU Berlin) for the support in OpenMS.

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Authors and Affiliations

  1. Physical Chemistry, University of Potsdam, Karl-Liebknecht-Straße 24-25, 14476, Potsdam, Germany

    José Villatoro, Martin Zühlke, Daniel Riebe, Toralf Beitz & Hans-Gerd Löhmannsröben

  2. Zuse Institute Berlin, Takustraße 7, D-14195, Berlin-Dahlem, Germany

    José Villatoro & Marcus Weber

  3. BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Straße 11, 12489, Berlin, Germany

    José Villatoro & Jens Riedel

  4. School of Analytical Sciences Adlershof (SALSA), Humboldt-Universität zu Berlin, Unter den Linden 6, 10099, Berlin, Germany

    José Villatoro

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  1. José Villatoro
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  2. Martin Zühlke
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  3. Daniel Riebe
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  4. Toralf Beitz
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  6. Jens Riedel
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  7. Hans-Gerd Löhmannsröben
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Correspondence to José Villatoro.

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Villatoro, J., Zühlke, M., Riebe, D. et al. IR-MALDI ion mobility spectrometry: physical source characterization and application as HPLC detector. Int. J. Ion Mobil. Spec. 19, 197–207 (2016). https://doi.org/10.1007/s12127-016-0208-1

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  • DOI: https://doi.org/10.1007/s12127-016-0208-1

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