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1H, 13C and 15N resonance assignments of the periplasmic signalling domain of HasR, a TonB-dependent outer membrane heme transporter

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Abstract

TonB-dependent transporters (TBDTs) are bacterial outer membrane proteins that internalize nutrients such as vitamin B12, metal complexes, heme, some carbohydrates, etc. In addition to their transport activity, several TBDTs are also involved in a signalling cascade from the cell surface into the cytoplasm, via their periplasmic signalling domain. Here we report the backbone and side chain resonance assignments of the signalling domain of HasR, a TonB-dependent outer membrane heme transporter from Serratia marcescens as a first step towards its structural study.

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Acknowledgments

This work was funded by the Institut Pasteur, the CNRS and a grant from the ANR (Agence Nationale de la Recherche, 08-BLAN-HEMETRANS). GCA was funded by postdoctoral fellowships from the Mairie de Paris and the ANR. IM was supported by a fellowship from the Ecole doctorale iViv and UPMC (Université Pierre et Marie Curie).

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Authors and Affiliations

  1. Institut Pasteur, Unité de RMN des Biomolécules, Département de Biologie Structurale et Chimie, 28, rue du Dr Roux, 75724, Paris Cedex 15, France

    Idir Malki, Gisele Cardoso de Amorim, Catherine Simenel, Ada Prochnicka-Chalufour, Muriel Delepierre & Nadia Izadi-Pruneyre

  2. CNRS, UMR 3528, 75015, Paris, France

    Idir Malki, Gisele Cardoso de Amorim, Catherine Simenel, Ada Prochnicka-Chalufour, Muriel Delepierre & Nadia Izadi-Pruneyre

  3. Université Pierre et Marie Curie, Cellule Pasteur UPMC, 75015, Paris, France

    Idir Malki

Authors
  1. Idir Malki
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  2. Gisele Cardoso de Amorim
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  3. Catherine Simenel
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  4. Ada Prochnicka-Chalufour
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  5. Muriel Delepierre
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  6. Nadia Izadi-Pruneyre
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Corresponding author

Correspondence to Nadia Izadi-Pruneyre.

Additional information

Idir Malki and Gisele Cardoso de Amorim contributed equally to this work.

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Malki, I., Cardoso de Amorim, G., Simenel, C. et al. 1H, 13C and 15N resonance assignments of the periplasmic signalling domain of HasR, a TonB-dependent outer membrane heme transporter. Biomol NMR Assign 7, 43–46 (2013). https://doi.org/10.1007/s12104-012-9377-y

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  • DOI: https://doi.org/10.1007/s12104-012-9377-y

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