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1H, 13C, 15N backbone and side-chain resonance assignment of Nostoc sp. C139A variant of the heme–nitric oxide/oxygen binding (H-NOX) domain

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Abstract

The H-NOX (Heme-nitric oxide/oxygen binding) domain is conserved across eukaryotes and bacteria. In human soluble guanylyl cyclase (sGC) the H-NOX domain functions as a sensor for the gaseous signaling agent nitric oxide (NO). sGC contains the heme-binding H-NOX domain at its N-terminus, which regulates the catalytic site contained within the C-terminal end of the enzyme catalyzing the conversion of GTP (guanosine 5′-triphosphate) to GMP (guanylyl monophosphate). Here, we present the backbone and side-chain assignments of the 1H, 13C and 15N resonances of the 183-residue H-NOX domain from Nostoc sp. through solution NMR.

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Abbreviations

NMR:

Nuclear Magnetic Resonance

HSQC:

Heteronuclear single quantum coherence

H-NOX:

Heme–nitric oxide/oxygen binding domain

sGC:

Soluble guanylyl cyclase

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Acknowledgments

"SEE-DRUG" Grant (EU FP7 REGPOT CT-2011-285950; www.seedrug.upatras.gr) is acknowledged for financial support.

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    Authors and Affiliations

    1. Department of Pharmacy, University of Patras, 26504, Patras, Greece

      Ioannis I. Alexandropoulos, Aikaterini I. Argyriou, Kostas D. Marousis & Georgios A. Spyroulias

    2. Department of Pharmacy, Laboratory of Molecular Pharmacology, University of Patras, 26504, Patras, Greece

      Stavros Topouzis & Andreas Papapetropoulos

    3. Faculty of Pharmacy, National and Kapodistrian University of Athens, 15 771, Athens, Greece

      Andreas Papapetropoulos

    Authors
    1. Ioannis I. Alexandropoulos
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    2. Aikaterini I. Argyriou
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    3. Kostas D. Marousis
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    4. Stavros Topouzis
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    5. Andreas Papapetropoulos
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    6. Georgios A. Spyroulias
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    Corresponding author

    Correspondence to Georgios A. Spyroulias.

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    Ioannis I. Alexandropoulos and Aikaterini I. Argyriou have contributed equally to this work.

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    Alexandropoulos, I.I., Argyriou, A.I., Marousis, K.D. et al. 1H, 13C, 15N backbone and side-chain resonance assignment of Nostoc sp. C139A variant of the heme–nitric oxide/oxygen binding (H-NOX) domain. Biomol NMR Assign 10, 395–400 (2016). https://doi.org/10.1007/s12104-016-9707-6

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    • DOI: https://doi.org/10.1007/s12104-016-9707-6

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