Abstract
CGC-19, a 14 kDa proteic constituent of a non ribosomal peptide synthetase implicated in the biosynthesis of a secondary metabolite in Streptomyces ambofaciens, has been isotopically enriched and recombinantly expressed. Its nearly complete 1H, 13C and 15N resonance assignment is reported hereunder.
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Acknowledgments
We thank Dr. J.L. Pernodet and Dr. S. Lautru (Institut de Génétique et Microbiologie, UMR 8621, CNRS/Université Paris-sud 11) for supplying us with the CGC-19 plasmid and for fruitful discussions and Dr. J.P. Lecaer for recording the mass spectra. ICSN is acknowledged for a research fellowship to SN.
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Nogaret, S., Guittet, E. & Birlirakis, N. 1H, 13C and 15N NMR assignment of CGC-19, a single domain proteic constituent of a non ribosomal peptide synthetase. Biomol NMR Assign 7, 1–4 (2013). https://doi.org/10.1007/s12104-012-9364-3
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DOI: https://doi.org/10.1007/s12104-012-9364-3