Abstract
The cDNA of UVI31+ was cloned from C. reinhardtii and expressed in E. coli from where the protein was purified to homogeneity. The purified protein exhibited beta-lactamase activity (Manuscript in preparation). However, UVI31+ has no homology with the known β-lactamases. In order to understand the structural basis of the ability of UVI31+ to hydrolyze β-lactam antibiotics, we in parallel, set out to structurally characterize it by NMR. Its β-lactamase activity in relation to the solution structure by NMR is likely to provoke deeper understanding of its mechanism and facilitate the rationalization of other functions of the protein, if any. In this endeavor, we report almost complete sequence-specific backbone 1H, 13C and 15N NMR assignments of UVI31+.
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The facilities provided by the National Facility for High Field NMR, supported by the Department of Science and Technology (DST), Department of Biotechnology (DBT), Council of Scientific and Industrial Research (CSIR), and Tata Institute of Fundamental Research, Mumbai, are gratefully acknowledged.
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Rout, A.K., Minda, R., Peri, D. et al. Sequence specific 1H, 13C and 15N backbone resonance assignments of UVI31+ from Chlamydomonas reinhardtii . Biomol NMR Assign 4, 171–174 (2010). https://doi.org/10.1007/s12104-010-9239-4
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DOI: https://doi.org/10.1007/s12104-010-9239-4