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hBolA, novel non-classical secreted proteins, belonging to different BolA family with functional divergence

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Abstract

This study reported that all three human BolA proteins (hBolA1, hBolA2, and hBolA3) are novel non-classical secreted proteins identified with bioinformatics and molecular biology experiments. The three BolA fusion proteins with c-Myc tag could be secreted into the culture medium of the transfected Cos-7 cells, although they could not be colocalized with Golgi apparatus. And the secretion of three BolA proteins could not be inhibited after BFA treatment. Furthermore, the secretion was not dependent on its predicted signal peptide. All the experiment results suggested that the secretion was a non-classical export. Phylogenetic analysis showed that the human BolAs belong to three different groups with functional divergence of BolA subfamily, where the different helix-turn-helix motif among hBolA1, hBolA2, and hBolA3 could be responsible for their functional divergence. Our data provided a basis for functional studies of BolA protein family.

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Abbreviations

HTH motif:

Helix-turn-helix motif

ER:

Endoplasmic reticulum

TGN:

Trans-Golgi network

SP:

Signal peptide

BFA:

Brefeldin A

AMW:

Apparent molecular weight

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Acknowledgments

This work was supported by Chinese High Tech R&D Program (2006AA02Z193), Chinese National Key Program on Basic Research (2004CB518605), National Natural Science Foundation of China (30425019), and Shanghai Commission for Science and Technology.

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Authors and Affiliations

  1. Chinese National Human Genome Center at Shanghai, 351 Guo Shou-Jing Road, Shanghai, 201203, China

    Yu-Bo Zhou, Jia-Bing Cao, Bing-Bing Wan, Xin-Rong Wang, Hong Zhu, Hong-Meng Yang, Ke-Sheng Wang, Xin Zhang & Ze-Guang Han

  2. Bioinformatics Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai, 200031, China

    Guo-Hui Ding

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  1. Yu-Bo Zhou
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  2. Jia-Bing Cao
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Corresponding author

Correspondence to Ze-Guang Han.

Additional information

Yu-Bo Zhou, Jia-Bing Cao, and Bing-Bing Wan contributed equally to this work.

Electronic Supplementary material

Supplement 1

Primary structure of hBolA1, hBolA2 and hBolA3. (A) Schematic representation of protein structure of hBolA1, hBolA2 and hBolA3. “SP” means signal peptide and HTH motif means helix-turn-helix motif. (B) Schematic representation of genomic structure of hBolA1, hBolA2 and hBolA3. (PDF 25 kb)

Supplement 2

Tissue expression profiles of hBolA1, hBolA2 and hBolA3 genes detected with RT-PCR. (PDF 31 kb)

Supplement 3

The AMW change of hBolAs treated without(-) or with(+) tunicamycin treatment. (PDF 18 kb)

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Zhou, YB., Cao, JB., Wan, BB. et al. hBolA, novel non-classical secreted proteins, belonging to different BolA family with functional divergence. Mol Cell Biochem 317, 61–68 (2008). https://doi.org/10.1007/s11010-008-9809-2

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  • DOI: https://doi.org/10.1007/s11010-008-9809-2

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