Skip to main content
Log in

1H, 13C and 15N resonance assignments for the active conformation of the small G protein RalB in complex with its effector RLIP76

  • Article
  • Published:
Biomolecular NMR Assignments Aims and scope Submit manuscript

Abstract

We report here the 1H, 15N and 13C resonance assignments for the small G protein RalB bound to the GTP analogue, GMPPNP and complexed with the Ral binding domain of its downstream effector RLIP76. The BMRB accession code is 15525.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Awasthi YC, Sharma R, Yadav S et al (2007) The non-ABC drug transporter RLIP76 (RALBP-1) plays a major role in the mechanisms of drug resistance. Curr Drug Metab 8:315–323. doi:10.2174/138920007780655414

    Article  Google Scholar 

  • Fukai S, Matern HT, Jagath JR et al (2003) Structural basis of the interaction between RalA and Sec5, a subunit of the Sec6/8 complex. EMBO J 22:3267–3278. doi:10.1093/emboj/cdg329

    Article  Google Scholar 

  • Ikeda M, Ishida O, Hinoi T, Kishida S, Kikuchi A (1998) Identification and characterization of a novel protein interacting with Ral-binding protein 1, a putative effector protein of Ral. J Biol Chem 273:814–821

    Article  Google Scholar 

  • Jin R, Junutula JR, Matern HT et al (2005) Exo84 and Sec5 are competitive regulatory Sec6/8 effectors to the RalA GTPase. EMBO J 24:2064–2074. doi:10.1038/sj.emboj.7600699

    Article  Google Scholar 

  • Jullien-Flores V, Mahé Y, Mirey G, Leprince C, Meunier-Bisceuil B, Sorkin A, Camonis JH (2000) RLIP76, an effector of the GTPase Ral, interacts with the AP2 complex: involvement of the Ral pathway in receptor endocytosis. J Cell Sci 113:2837–2844

    Google Scholar 

  • Neidhardt FC, Bloch PL, Smith DF (1974) Culture medium for enterobacteria. J Bacteriol 119:736–747

    Google Scholar 

  • Nicely NI, Kosak J, de Serrano V et al (2004) Crystal structures of Ral-GppNHp and Ral-GDP reveal two binding sites that are also present in Ras and Rap. Structure 12:2025–2036. doi:10.1016/j.str.2004.08.011

    Article  Google Scholar 

  • Prasannan S, Fenwick RB, Campbell LJ et al (2007) 1H, 13C, and 15N resonance assignments for the small G protein RalB in its active conformation. Biomol NMR Assign 1:147–149. doi:10.1007/s12104-007-9040-1

    Article  Google Scholar 

  • Sattler M, Schleucher J, Griesinger C (1999) Heteronuclear multidimensional NMR experiments for the structure determination of proteins in solution employing pulsed field gradients. Prog Nucl Magn Reson Spectrosc 34:93–158. doi:10.1016/S0079-6565(98)00025-9

    Article  Google Scholar 

  • Shipitsin M, Feig LA (2004) RalA but not RalB enhances polarized delivery of membrane proteins to the basolateral surface of epithelial cells. Mol Cell Biol 24:5746–5756. doi:10.1128/MCB.24.13.5746-5756.2004

    Article  Google Scholar 

  • van Dam EM, Robinson PJ (2006) Ral: mediator of membrane trafficking. Int J Biochem Cell Biol 38:1841–1847. doi:10.1016/j.biocel.2006.04.006

    Article  Google Scholar 

  • Vranken WF, Boucher W, Stevens TJ et al (2005) The CCPN data model for NMR spectroscopy: development of a software pipeline. Proteins 59:687–696. doi:10.1002/prot.20449

    Article  Google Scholar 

  • Yamaguchi A, Urano T, Goi T, Feig LA (1997) An Eps homology (EH) domain protein that binds to the Ral-GTPase target, RalBP1. J Biol Chem 272:31230–31234

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by Cancer Research UK, grant C9467/A4658. RBF was supported by the Cambridge Commonwealth Trust and the Overseas Research Students Awards Scheme.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Helen R. Mott.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bryn Fenwick, R., Prasannan, S., Campbell, L.J. et al. 1H, 13C and 15N resonance assignments for the active conformation of the small G protein RalB in complex with its effector RLIP76. Biomol NMR Assign 2, 179–182 (2008). https://doi.org/10.1007/s12104-008-9115-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12104-008-9115-7

Keywords

Navigation