Skip to main content
SpringerLink
Log in

Comet assay in neonatal sepsis

  • Original Article
  • Published:
The Indian Journal of Pediatrics Aims and scope Submit manuscript

Abstract

Objective

To determine whether the DNA damage detected using the Comet assay helps in the diagnosis of neonatal sepsis

Methods

Sepsis was diagnosed, based on clinical findings, laboratory findings and positive culture results, in 24 of the 48 newborns who participated in the study. Hematological parameters, baseline demographic data and genotoxic evaluation were investigated.

Results

There were no significant differences in hematological, demographic and genotoxic data between cases and controls.

Conclusions

The Comet assay proved to be ineffective in the diagnosis of neonatal sepsis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Vergnano S, Sharland M, Kazembe P, Mwansambo C, Heath PT. Neonatal sepsis: an international perspective. Arch Dis Child Fetal Neonatal Ed 2005; 90: 220–224.

    Article  Google Scholar 

  2. Goldstein B, Giroir B, Randolph A. International pediatric sepsis consensus conference: Definitions for sepsis and organ dysfunction in pediatrics. Pediatr Crit Care Med 2005; 6: 2–8.

    Article  PubMed  Google Scholar 

  3. Ersoy B, Nehir H, Altinoz S, Yilmaz O, Dundar PE, Aydogan A. Prognostic of initial antithrombin levels in neonatal sepsis. Indian Pediatr 2007; 44: 581–584.

    PubMed  Google Scholar 

  4. Kingsmore SF, Kennedy N, Halliday HL et al. Identification of diagnostic biomarkers for infection in premature neonates. Mol Cell Proteomics 2008; 10: 1863–1875.

    Google Scholar 

  5. Arnon S, Litmanovitz I. Diagnostic tests in neonatal sepsis. Curr Opin Infect Dis 2008; 21: 223–227.

    Article  PubMed  Google Scholar 

  6. Cancelier AC, Petronilho F, Reinke A et al. Inflammatory and oxidative parameters in cord blood as diagnostic of earlyonset neonatal sepsis: a case-control study. Pediatr Crit Care Med 2009; 10: 467–471.

    Article  PubMed  Google Scholar 

  7. Dusinska M, Collins AR. The comet assay in human biomonitoring: gene-environment interactions. Mutagenesis 2008; 23: 191–205.

    Article  PubMed  CAS  Google Scholar 

  8. Tice RR, Agurell E, Anderson D et al. Single-cell gel/Comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 2000; 35: 206–221.

    Article  PubMed  CAS  Google Scholar 

  9. Villela IV, Oliveira IM, Silva J, Henriques JAP. DNA damage and repair in haemolymph cells of golden mussel (Limnoperna fortunei) exposed to environmental contaminants. Mutat Res 2006; 605: 78–86.

    PubMed  CAS  Google Scholar 

  10. Faust F, Kassie F, Knasmuller S, Boedecker RH, Mann M, Mersch-Sundermann V. The use of the alkaline comet assay with lymphocytes in human biomonitoring studies. Mutat Res 2004; 566: 209–229.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratório de Imunologia e Mutagênese, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil

    Natália C. Carvalho, Rozilda L. de Souza & Vanessa Moraes de Andrade

  2. Laboratório de Fisiopatologia Experimental, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil

    Felipe Dal-Pizzol

  3. Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense (UNESC), 88806-000, Criciúma - SC, Brazil

    Vanessa Moraes de Andrade

Authors
  1. Natália C. Carvalho
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rozilda L. de Souza
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Felipe Dal-Pizzol
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Vanessa Moraes de Andrade
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Vanessa Moraes de Andrade.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Carvalho, N.C., de Souza, R.L., Dal-Pizzol, F. et al. Comet assay in neonatal sepsis. Indian J Pediatr 77, 875–877 (2010). https://doi.org/10.1007/s12098-010-0127-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12098-010-0127-9

Key words

Search

Navigation