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Geobacillus thermoleovorans MTCC 13131: An Amide-Hydrolyzing Thermophilic Bacterium Isolated from a Hot Spring of Manikaran

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Abstract

Geobacillus thermoleovorans MTCC 13131, an amide hydrolyzing bacteria was isolated from a hot spring in Himachal Pradesh and identified through 16S rRNA gene sequence analysis. The amidase derived from this bacterium exhibited hydrolyzing catalytic ability against aliphatic and aromatic amides. The isolate was characterized for morphological and biochemical properties. Further, the production of amidase enzyme from this isolate was evaluated using approach of one-variable-at-a-time and response surface method. The Response Surface Methodology based study indicated the importance of nitrogen sources and growth period for amidase production. Optimal production was achieved at a temperature 55 °C, and production pH 7.5 in the production medium comprising diammonium hydrogen phosphate (0.4%), peptone (0.45%) and yeast extract (0.3%). The wide substrate affinity of the strain suggests its potential role in biotransformation of amides to corresponding acids of industrial significance along with its strong capacity to degrade the toxic amide in polluted environmental samples.

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Acknowledgements

Dr. Praveen Kumar Mehta is thankful to the Science and Engineering Research Board (SERB) for providing financial support to the laboratory as an Early Carrier Research grant (ECR/2017/000980), New Delhi, Government of India.

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Authors and Affiliations

  1. Center for Molecular Biology, Central University of Jammu, Bagla, Rahya Suchani, Jammu, Jammu and Kashmir, 181143, India

    Arun Kumar, Refana Shahul, Ashok Kumar & Praveen Kumar Mehta

  2. Environmental Energy Engineering, College of Engineering, University of Suwon, Hwaseong, 18323, Republic of Korea

    Rajendra Singh

  3. Department of Life Sciences, School of Basics Sciences and Research, Sharda University, Greater Noida, Uttar Pradesh, 201310, India

    Sanjay Kumar

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  1. Arun Kumar
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Kumar, A., Shahul, R., Singh, R. et al. Geobacillus thermoleovorans MTCC 13131: An Amide-Hydrolyzing Thermophilic Bacterium Isolated from a Hot Spring of Manikaran. Indian J Microbiol 62, 618–626 (2022). https://doi.org/10.1007/s12088-022-01042-9

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