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The Core Role of Bacillus subtilis and Aspergillus fumigatus in Pile-Fermentation Processing of Qingzhuan Brick Tea

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Abstract

To identify the microorganisms responsible for the formation of the main quality components of Qingzhuan brick tea (QZBT) during solid-state fermentation (SSF), predominant thermoduric strains were isolated from the tea leaves collected during SSF. According to their capability of releasing cellulase, pectase, protease, and polyphenol oxidase, four strains were selected as starter cultures to ferment sun-dried tea leaves during artificially inoculated SSF. According to the major enzymatic activities and quality components content (tea polyphenols, catechins, amino acids, soluble sugar, and theabrownin), it was found that Aspergillus fumigatus M1 had a significant effect on the transformation of polyphenols and Bacillus subtilis X4 could enhance the ability of bioconversion of strain M1. Strain X4 and M1 may be the core microbes responsible for developing these biochemical components of QZBT, as the values of quality components of tea leaves fermented by these two strains for 6 days was very close to that of the sample naturally fermented for 35 days in the tea factory. The results could be significant in developing industrial starters for the manufacture of QZBT and stabilizing the product quality of different batches.

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References

  1. Lv HP, Zhang YJ, Lin Z, Liang YR (2013) Processing and chemical constituents of Pu-erh tea: a Review. Food Res Int 53:608–618

    Article  CAS  Google Scholar 

  2. Liu SY, Yu Z, Zhu HK, Zhang W, Chen YQ (2016) In vitro α-glucosidase inhibitory activity of isolated fractions from water extract of Qingzhuan dark tea. BMC Complement Altern M 16:378–386

    Article  CAS  Google Scholar 

  3. Yang XH, Huang MJ, Qin CQ, Lv BY, Mao QL, Liu ZH (2017) Structural characterization and evaluation of the antioxidant activities of polysaccharides extracted from Qingzhuan brick tea. Int J Biol Macromol 101:768–775

    Article  CAS  PubMed  Google Scholar 

  4. Liu PP, Zheng PC, Gong ZM, Wang SP, Teng J, Gao SW, Wang XP, Ye F, Zheng L (2017) Analysis of aroma components in Qingzhuan dark tea. Food Sci 38:164–170

    Article  Google Scholar 

  5. Zheng PC, Tan RR, Liu PP, Wang SP, Teng J, Wang XP, Gong ZM (2017) Study on the fungi species and quality changes of green brick tea during pile-fermentation process. Food Sci Technol 42:22–26

    Google Scholar 

  6. Liu TT, Qi GN, Zou Y, Li W, Huang YF (2015) Variations in quality ingredients and primary enzymes activities of Sichuan dark tea during post fermentation. J South China Agric Univ 36:112–116

    CAS  Google Scholar 

  7. Wang Q, Gong J, Chisti Y, Sirisansaneeyakul S (2014) Bioconversion of tea polyphenols to bioactive theabrownins by Aspergillus fumigatus. Biotech Lett 36:2515–2522

    Article  CAS  Google Scholar 

  8. Ma Y, Duan SM, Zhang DL, Su XQ, Zhang DY, Lv CY, Zhao M (2017) Microbialsuccession and the dynamics of chemical compounds during the solid-state fermentation of Pu-erh tea. Appl Sci 7:166–182

    Article  CAS  Google Scholar 

  9. Gong JS, Zhou HJ, Zhang XF, Song S, An WJ (2005) Changes of chemical components in Pu’er tea produced by solid state fermentation of sundried green Tea. J Tea Sci 25:300–306

    CAS  Google Scholar 

  10. Gu S, Chen JL, Wang Y (2017) Mold DNA amplification from traditional mold fermented foods. Food Sci 38:83–86

    Google Scholar 

  11. Zhao M, Zhang DL, Su XQ, Duan SM, Wan JQ, Yuan WX, Liu BY, Ma Y, Pan YH (2015) Anintegrated metagenomics/metaproteomics investigation of the microbial communities and enzymes in solid-state fermentation of Pu-erh tea. Sci Rep 5:10–17

    Google Scholar 

  12. Chen YY, Fu XM, Mei X, Zhou Y, Cheng SH, Zeng LT, Dong F, Yang ZY (2017) Proteolysis of chloroplast proteins is responsible for accumulation of free amino acids in dark-treated tea (Camellia sinensis) leaves. J Proteomics 157:10–17

    Article  CAS  PubMed  Google Scholar 

  13. Si MR, Xue QH, Cai Y (2002) The effect of mixed fermentation on cellulase and amylase activities. J Northwest Sci-Tech Univ Agric For 30:69–73

    Google Scholar 

  14. Li HZ, Li M, Yang XR, Gui X, Chen GF, Chu JY, He XW, Wang WT, Han F, Li P (2018) Microbial diversity and component variation in Xiaguan Tuo Tea during pile fermentation. PLoS ONE 13:e0190318

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

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Correspondence to Ruiyan Fu.

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Xu, Q., Sun, M., Ning, J. et al. The Core Role of Bacillus subtilis and Aspergillus fumigatus in Pile-Fermentation Processing of Qingzhuan Brick Tea. Indian J Microbiol 59, 288–294 (2019). https://doi.org/10.1007/s12088-019-00802-4

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  • DOI: https://doi.org/10.1007/s12088-019-00802-4

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