Abstract
Fish processing industries generate large quantities of fish scales as processing waste, if not treated leading to environmental pollution. Fish scales are hard to degrade, hence cause difficulty in waste management. In this context present study was made to utilize fish scales as substrate for the production of alkaline protease by Bacillus altitudinis GVC11 and subsequently amino acid rich aqua hydrolyzate. B. altitudinis GVC11 efficiently utilized five types of fish scales as substrates and produced maximum alkaline protease using Labeo rohita (28,150 U/mL) followed by Catla catla (23,320 U/mL) at 48 h and Cyprinus carpio (17,146 U/mL) Mugil cephalus (18,917 U/mL), Cirrhinus mrigala (12,430 U/mL) at 72 h. The HPLC analysis of protein hydrolyzate obtained after fermentation was enriched in essential amino acids, leucine, isoleucine, phenylalanine, lysine and non-essential amino acids, tyrosine, arginine and cysteine which can be used as animal feed supplement and organic fertilizer.
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References
FAO (2016) Food outlook, biannual report on global food markets. In: Food and agriculture organization of the United Nations, pp 60–66
Rahmi M, Faid M, EiYachioui M, Berny EH, Fakir M, Ouhssine M (2008) Protein rich ingredients from fish waste for sheep feeding. Afr J Microbiol 2:73–77
Esakkiraj P, Maruthiah T, Nawas PMA, Immanuel G, Palavesam A (2013) Solid state protease production by Bacillus thuringiensis AP-CMST using trash fish meal as substrate. J Pure Appl Microbiol 7:1–8
Jayathilakan P, Sultana K, Radhakrishna K, Bawa KS (2012) Utilization of byproducts and waste materials from meat, poultry and fish processing industries: a review. J Food Sci Technol 49:278–293. doi:10.1007/s13197-011-0290-7
Pan MH, Tsai ML, Chen WM, Hwang A, Pan BS, Hwang YR, Kuo JM (2010) Purification and characterization of a fish scale-degrading enzyme from a newly identified Vogesella sp. J Agric Food Chem 58:12541–12546. doi:10.1021/jf1034042
Basu B, Ajit KB (2005) Production of protein rich organic fertilizer from fish scale by Aspergillus mutant AB100-A media optimization study. J Sci Ind Res 64:293–298. doi:10.1007/s11274-007-9492-6
Matmaroh K, Benjakul B, Prodpran T, Encarnacion AB, Kishimura H (2011) Characteristics of acid soluble collagen and pepsin soluble collagen from scale of spotted golden goatfish (Parupeneus heptacanthus). Food Chem 129:1179–1186. doi:10.1016/j.foodchem.2011.05.099
Pati F, Adhikari B, Dhara S (2010) Isolation and characterization of fish scale collagen of higher thermal stability. Biores Technol 101:3737–3742. doi:10.1016/j.biortech.2009.12.133
Mahboob S (2015) Isolation and characterization of collagen from fish waste material-skin, scales and fins of Catla catla and Cirrhinus mrigala. J Food Sci Technol 52:4296–4305. doi:10.1007/s13197-014-1520-6
Kasana RC, Salwan R, Yadav SK (2011) Microbial proteases: detection, production, and genetic improvement. Crit Rev Microbiol 37:262–276. doi:10.3109/1040841X.2011.577029
Sanatan PT, Lomate PR, Giri AP, Hivrale VK (2013) Characterization of a chemostable serine alkaline protease from Periplaneta americana. BMC Biochem 14:1–9. doi:10.1186/1471-2091-14-32
Souissi N, Ellouz-Triki Y, Bougatef A, Blibech M, Nasri M (2008) Preparation and use of media for protease producing bacterial strains based on by-products from Cuttlefish (Sepia officinalis) and wastewaters from marine-products processing factories. Microbiol Res 163:473–480. doi:10.1016/j.micres.2006.07.013
Mukherjee AK, Adhikari H, Rai SK (2008) Production of alkaline protease by a thermophilic Bacillus subtilis under solid-state fermentation SSF condition using Imperata cylindrica grass and potato peel as low-cost medium: characterization and application of enzyme in detergent formulation. Biochem Eng J 39:353–361. doi:10.1016/j.bej.2007.09.017
Srividya S, Mala M (2011) Influence of process parameters on the production of detergent compatible alkaline protease by a newly isolated Bacillus sp. Y. Turk J Biol 35:177–182. doi:10.3906/biy-0906-47
AOAC (2009) Protein (crude) determination in animal feed: copper catalyst Kjeldahl method 984.13, 15th edn. Official methods of analysis of AOAC International, Gaithersburg
Vijay Kumar E, Srijana M, Kiran Kumar K, Harikrishna N, Reddy Gopal (2011) A novel serine alkaline protease from Bacillus altitudinis GVC11and its application as a dehairing agent. Bioprocess Biosyst Eng 34:403–409. doi:10.1007/s00449-010-0483-x
Shi Z, Li H, Li Z, Hu J, Zhang H (2013) Pre-column derivatization RP-HPLC determination of amino acids in Asparagi radix before and after heating process. In: Proceedings of 15 international conference on agricultural and natural resources engineering. 24–25 Dec 2013. pp 351–356
Rebah FB, Miled N (2013) Fish processing wastes for microbial enzyme production: a review. 3 Biotech 3:255–265. doi:10.1007/s13205-012-0099-8
Zhang F, Wang Z, Shiying X (2009) Macroporous resin purification of grass carp fish (Ctenopharyngodon idella) scale peptides with in vitro angiotensin-I converting enzyme (ACE) inhibitory ability. Food Chem 117:387–392. doi:10.1016/j.foodchem.2009.04.015
Zhang F, Wang A, Li Z, He S, Shao L (2011) Preparation and characterization of collagen from fresh water fish scales. Food Nutr Sci 2:818–823. doi:10.4236/fns.2011.28112
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Authors thank the DBT- ISLARE for financial support to carry out this work and fellowship to HK.
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Harikrishna, N., Mahalakshmi, S., Kiran Kumar, K. et al. Fish Scales as Potential Substrate for Production of Alkaline Protease and Amino Acid Rich Aqua Hydrolyzate by Bacillus altitudinis GVC11. Indian J Microbiol 57, 339–343 (2017). https://doi.org/10.1007/s12088-017-0664-2
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DOI: https://doi.org/10.1007/s12088-017-0664-2