Abstract
In this work, acetic acid was found as one promising substrate to improve xylose utilization by Gluconacetobacter xylinus CH001. Also, with the help of adding acetic acid into medium, the bacterial cellulose (BC) production by G. xylinus was increased significantly. In the medium containing 3 g l−1 acetic acid, the optimal xylose concentration for BC production was 20 g l−1. In the medium containing 20 g l−1 xylose, the xylose utilization and BC production by G. xylinus were stimulated by acetic acid within certain concentration. The highest BC yield (1.35 ± 0.06 g l−1) was obtained in the medium containing 20 g l−1 xylose and 3 g l−1 acetic acid after 14 days. This value was 6.17-fold higher than the yield (0.21 ± 0.01 g l−1) in the medium only containing 20 g l−1 xylose. The results analyzed by FE-SEM, FTIR, and XRD showed that acetic acid affected little on the microscopic morphology and physicochemical characteristics of BC. Base on the phenomenon observed, lignocellulosic acid hydrolysates (xylose and acetic acid are main carbon sources present in it) could be considered as one potential substrate for BC production.
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Chawla PR, Bajaj IB, Survase SA, Singhal RS (2009) Microbial cellulose: fermentative production and applications. Food Technol Biotechnol 47:107–124
Bae S, Shoda M (2004) Bacterial cellulose production by fed-batch fermentation in molasses medium. Biotechnol Prog 20:1366–1371
Hong F, Qiu K (2008) An alternative carbon source from konjac powder for enhancing production of bacterial cellulose in static cultures by a model strain Acetobacter aceti subsp. xylinus ATCC 23770. Carbohydr Polym 72:545–549
Kurosumi A, Sasaki C, Yamashita Y, Nakamura Y (2009) Utilization of various fruit juices as carbon source for production of bacterial cellulose by Acetobacter xylinum NBRC 13693. Carbohydr Polym 76:333–335
Palmqvist E, Hahn-Hagerdal B (2000) Fermentation of lignocellulosic hydrolysates. II: Inhibitors and mechanisms of inhibition. Bioresour Technol 74:25–33
Ishihara M, Matsunaga M, Hayashi N, Tisler V (2002) Utilization of d-xylose as carbon source for production of bacterial cellulose. Enzym Microb Technol 31:986–991
Toda K, Asakura T, Fukaya M, Entani E, Kawamura Y (1997) Cellulose production by acetic acid-resistant Acetobacter xylinum. J Ferment Bioeng 84:228–231
Shen W, Chen S, Shi S, Li X, Zhang X, Hu W, Wang H (2009) Adsorption of Cu(II) and Pb(II) onto diethylenetriamine-bacterial cellulose. Carbohydr Polym 75:110–114
Shezad O, Khan S, Khan T, Park JK (2010) Physicochemical and mechanical characterization of bacterial cellulose produced with an excellent productivity in static conditions using a simple fed-batch cultivation strategy. Carbohydr Polym 82:173–180
Cheng KC, Catchmark JM, Demirci A (2009) Effect of different additives on bacterial cellulose production by Acetobacter xylinum and analysis of material property. Cellulose 16:1033–1045
Chen XF, Huang C, Xiong L, Chen XD, Ma LL (2012) Microbial oil production from corncob acid hydrolysate by Trichosporon cutaneum. Biotechnol Lett 34:1025–1028
Huang C, Chen XF, Xiong L, Chen XD, Ma LL (2012) Oil production by the yeast Trichosporon dermatis cultured in enzymatic hydrolysates of corncobs. Bioresour Technol 110:711–714
Nelson ML, O’Connor RT (1964) Relation of certain infrared bands to cellulose crystallinity and crystal latticed type. Part I. Spectra of lattice types I, II, III and of amorphous cellulose. J Appl Polym Sci 8:1311–1324
Yudianti R, Indrarti L, Azuma J (2007) Structure and physical properties of natural gellous materials. J Appl Sci 7:580–584
Acknowledgments
The authors acknowledge the financial support of Project of National Natural Science Foundation of China (51303181, 51378486, U1261116), Project of Jiangsu Province Science and Technology (BE2013083), Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences (y407pb1001, y107rf1001), Guangzhou Science and Technology (2013J4300031), the Support Plan Project of National Science and Technology (2012BAD32B07) and Natural Science Foundation of Guangdong Province (S2012040007546).
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Yang, XY., Huang, C., Guo, HJ. et al. Beneficial Effect of Acetic Acid on the Xylose Utilization and Bacterial Cellulose Production by Gluconacetobacter xylinus . Indian J Microbiol 54, 268–273 (2014). https://doi.org/10.1007/s12088-014-0450-3
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DOI: https://doi.org/10.1007/s12088-014-0450-3