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Prevalence of Microsporidium and Other Intestinal Parasites in Children from Malatya, Turkey

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Abstract

Parasite infections are common during the critical developmental period in children. The occurrences of intestinal parasites are also common in orphanage, nurseries and schools in Turkey. The study was carried out to determine the percentage of microsporidium and intestinal parasites in children from Malatya, Turkey. This study was carried out at the Department of Parasitology of Inonu University, Turgut Ozal Medical Center, during January–December 2006. Totally, 1,181 stool samples were examined using the native-Lugol, sedimentation-techniques, modified trichrome (MTS), acid-fast-trichrome stain and calcofluor staining methods. In addition, perianal region material was taken from the children to examine with cellophane tape method. Power analyses were performed for statistical analyses used. Microsporidia were found in 92 (7.8%) of the samples, and also intestinal parasites were detected in 329 (27.8%). The numbers of infections according to the species were as follows: 69 (5.8%) Entamoeba coli, 7 (0.6%) Blastocystis hominis, 114 (9.7%) Giardia intestinalis, 15 (1.3%) Iodomoeba butchlii, 8 (0.7%) Dientamoeba fragilis, 7 (0.6%) Taenia spp. 70 (5.9%) Enterobius vermicularis, 11 (0.9%) Hymenolepis nana, 25 (2.1%) Trichomonas intestinalis, 1 (0.1%) Ascaris lumbricoides and 2 (0.2%) Chilomastix mesnilii. Also, greater than 90% power values were achieved for statistical analyses. Whereas the detection rates of microsporidium and intestinal parasites were found to be low, it was concluded that in addition to intestinal parasites, microsporidium should be also searched for in children with complaints of intestinal system.

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References

  1. Shadduck JA (1989) Human microsporidiosis and AIDS. Rev Infect Dis 11:203

    Article  PubMed  CAS  Google Scholar 

  2. Shadduck JA, Greeley E (1989) Microsporidia and human infections. Clin Microbiol Rev 2:158–165

    PubMed  CAS  Google Scholar 

  3. Franzen C, Müller A (1999) Molecular techniques for detection, species differentiation, and phylogenetic analysis of microsporidia. Clin Microbiol Rev 12:243–285

    PubMed  CAS  Google Scholar 

  4. Eeftinck Schattenkerk JKMT, Van Gool T (1992) Clinical and microbiological aspects of microsporidiosis. Trop Geogr Med 44:287

    PubMed  CAS  Google Scholar 

  5. Franzen C, Müller A, Salzberger B, Fätkenheuer G, Eidt S, Mahrle G, Diehl V, Schrappe M (1995) Tissue diagnosis of intestinal microsporidiosis using a fluorescent stain with Uvitex 2B. J Clin Pathol 48:1009–1010

    Article  PubMed  CAS  Google Scholar 

  6. Caramello P, Mazzucco G, Romeo M, Ullio A, DeRosa G, Lucchini A, Forno B, Brancale T, Macor A, Preziosi C et al (1995) Clinical and microscopical features of small-intestinal microsporidiosis in patients with AIDS. Infection 23:362–368

    Article  PubMed  CAS  Google Scholar 

  7. Tumwine JK, Kekitiinwa A, Nabukeera N, Akiyoshi DE, Buckholt MA, Tzipori S (2002) Enterocytozoon bieneusi among children with diarrhea attending Mulago Hospital in Uganda. Am J Med Hyg 67(3):299–303

    Google Scholar 

  8. Termmathurapoj S, Engkanun K, Naaglor T, Taamsri P, Wirote A, Leelayoova S, Mungthin M (2000) Cross-sectional study of intestinal protozoan infections in orphans and childcare workers at the Phayathai babies’ home, Bangkok, Thailand. J Trop Med Parasitol 23:21–27

    Google Scholar 

  9. Yazar S, Eser B, Yalcin S, Sahin I, Koc AN (2003) A case of pulmonary microsporidiasis in an acute myeloblastic leukemia (AML)—M3 patient. Yonsei Med J 44(1):146–149

    PubMed  Google Scholar 

  10. Buget E, Buyukbaba-Boral O, Kirkoyun-Uysal H, Nazlican O, Ogut T, Sengur G (2000) First case report in Turkey: microsporidiosis and pulmonary cryptosporidiosis in an AIDS patient. T J Micro Soc 30(3–4):166–170

    Google Scholar 

  11. Ozkirim A, Keskin N (2001) A survey of Nosema apis of honey bees (Apis mellifera L.) producing the famous Anzer honey in Turkey. Z Naturforsch [C] 56(9–10):918–919

    CAS  Google Scholar 

  12. Eroksüz H, Eroksüz Y, Metin N, Ozer H (1999) Bir tavşan kolonisindeki doğal encephalitozoonosisolgları üzrine morfolojik incelemeler. Turk J Vet Anim Sci 23:191–195

    Google Scholar 

  13. Yaman M, Radek R (2003) Nosema chaetocnemae sp.n. (Microspora:Nosematidae), a microsporidian parasite of Chaetocnema tibialis (Coleoptera:Chrysomelidae). Acta Protozool 42:231–237

    Google Scholar 

  14. Jerrold HZ (1999) Biostatistical analysis, 4th edn. Prentice-Hall Inc, New Jersey. ISBN-10: 013081542X, ISBN-13: 9780130815422

  15. Yilmaz H, Göz Y, Bozkurt H (1999) Distribution of intestinal parasites in children from the 23 Nisan Primary School in Hakkari. Acta Parazitol Turcica 23(1):28–31

    Google Scholar 

  16. Kokoskin E, Gyorkos TW, Camus A, Cedilotte L, Purtill T, Ward B (1994) Modified technique for efficient detection of microsporidia. J Clin Microbiol 32(4):1974–1975

    Google Scholar 

  17. DeGirolami PC, Ezratty CR, Desai G, McCullough A, Asmuth D, Wanke C, Federman M (1995) Diagnosis of intestinal microsporidiosis by examination of stool and duodenal aspirate with Weber’s modified trichrome and Uvitex 2B strains. J Clin Microbiol 33(4):805–810

    PubMed  CAS  Google Scholar 

  18. Raynaud L, Delbac F, Broussolle V, Rabodonirina M, Girault V, Wallon M, Cozon G, Vivares CP, Peyron F (1998) Identification of Encephalitozoon intestinalis in travelers with chronic diarrhea by specific PCR amplification. J Clin Microbiol 36(1):37–40

    PubMed  CAS  Google Scholar 

  19. Carter PL, MacPherso DW, McKenzie RA (1996) Modified technique to recover microsporidian spores in sodium acetate-acetic acid-formalin-fixed fecal samples by light microscopy and correlation with transmission electron microscopy. J Clin Microbiol 34(11):2670–2673

    PubMed  CAS  Google Scholar 

  20. Tanyüksel M, Gün H (1995) Mikrosporidia. Acta Parazitol Turcica 19(2):200–209

    Google Scholar 

  21. Leelayoova S, Vithayasai N, Watanaveeradej V, Chotpitayasunondh T, Therapong V, Naaglor T, Mungthin M (2001) Intestinal microsporidiosis in HIV-infected children with acute and chronic diarrhea. Southeast Asian J Trop Med Public Health 32(1):33–37

    PubMed  CAS  Google Scholar 

  22. Bretagne S, Foulet F, Alkassoum W, Fleury-Feith J, Develoux M (1993) Prevalence of Enterocytozoon bieneusi spores in the stool of AIDS patients and African children not infected by HIV. Bull Soc Pathol Exot 86(5):351–357

    PubMed  CAS  Google Scholar 

  23. Durmaz B, Yakinci C, Rafiq M, Durmaz R (1997) The prevalence of intestinal parasites among orphans and primary school children in Malatya. Acta Parazitol Turcica 21:391–394

    Google Scholar 

  24. Günes G, Celik T, Regiq M, Kaya M, Pehlivan E, Daldal N (2000) Parasitological investigations of children and staff in orphanages in Malatya. Acta Parazitol Turcica 24:290–293

    Google Scholar 

  25. Zeyrek FY, Ozbilge H, Zeyrek CD (2003) Prevalence of intestinal parasitic infections among nursery school and orphanage. Acta Parazitol Turcica 27(2):133–135

    Google Scholar 

  26. Celiksöz A, Demirtas S, Sümer Z, Özcelik S, Saygı G (1997) A survey of intestinal parasites in orphan of orphanage in Sivas. Acta Parazitol Turcica 21:45–47

    Google Scholar 

  27. Ercevik HE, Idil A (2002) Prevalence of intestinal parasites and associated factors of this into primary school which have different socioeconomic level. T Klin J Med Sci 22:113–118

    Google Scholar 

  28. Didier ES, Stovall ME, Green LC, Brindley PJ, Sestak K, Didier PJ (2004) Epidemiology of microsporidiosis: sources and modes of transmission. Vet Parasitol 126:145–166

    Article  PubMed  CAS  Google Scholar 

  29. John DE, Haas CN, Nwachuku N, Gerba CP (2005) Chlorine and ozone disinfection of Encephalitozoon intestinalis spores. Water Res 39(11):2369–2375

    Article  PubMed  CAS  Google Scholar 

  30. Karaman U, Daldal N, Atambay M, Colak C (2009) The epidemiology of microsporidias in human (Malatya sample). T J Med Sci 39(2):281–288

    Google Scholar 

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Acknowledgments

We thank to Prof. Dr. Rainer Weber, Prof. Dr. Lynne S. Garcia, Prof. Dr. Nilgun Daldal and Assoc. Prof. Dr. Mustafa Yaman for the contributions of this study.

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Authors and Affiliations

  1. Department of Statistics, Firat University, 23119, Elazig, Turkey

    Sinan Calik

  2. Health High School, Ordu University, 52100, Ordu, Turkey

    Ulku Karaman

  3. Department of Biostatistics, Inonu University, 44280, Malatya, Turkey

    Cemil Colak

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  1. Sinan Calik
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  2. Ulku Karaman
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  3. Cemil Colak
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Correspondence to Ulku Karaman.

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Calik, S., Karaman, U. & Colak, C. Prevalence of Microsporidium and Other Intestinal Parasites in Children from Malatya, Turkey. Indian J Microbiol 51, 345–349 (2011). https://doi.org/10.1007/s12088-011-0107-4

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  • DOI: https://doi.org/10.1007/s12088-011-0107-4

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