Skip to main content
SpringerLink
Log in

Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions

  • Original Article
  • Published:
Indian Journal of Microbiology Aims and scope Submit manuscript

Abstract

Aspergillus terreus produces lipase 7.01 IU/ml in 96 h after optimization by one variable at a time method. Using the significant factors i.e. corn oil (A), sodium nitrate (B), casein (C), agitation rate (D) and incubation period (E) RSM was carried out resulting in 19.65 IU/ml from the combination +1(A), −1(B), −1(C), +1(D) and 0(E). The interactions between sodium nitrate, casein and agitation with corn oil were most significant. Scale up of production from 250 ml shake flask to 30 l bioreactor resulted in increased productivity of 0.52 IU/ml/h as against 0.2 IU/ml/h obtained in shake flasks. This lipase could carryout solvent free synthesis of partial glycerides of oleic acid with 96% efficiency in 12 h.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Abbreviations

RSM:

Response surface methodology

FCCCD:

Face centered central composite design

OVAT:

One variable at a time

References

  1. Kumar SS, Gupta R (2008) An extracellular lipase from Trichosporon asahii MSR 54: medium optimization and enantioselective deacetylation of phenyl ethyl acetate. Process Biochem 43:1054–1060

    Article  CAS  Google Scholar 

  2. Saxena RK, Ghosh PK, Gupta R, Davidson WS, Bradoo S, Gulati R (1999) Microbial lipases: potential biocatalyst for the future industry. Curr Sci 77:101–115

    CAS  Google Scholar 

  3. Saxena RK, Agarwal L, Meghwanshi GK (2005) In: Satyanaryana T, Johri BN (eds) Diversity of fungal and yeast lipases: present in future scenario for 21st century. I.K. International Pvt. Ltd., New Delhi, pp 796–841

  4. Gupta R, Gupta N, Rathi P (2004) Bacterial lipases: an overview of production, purification and biochemical properties. Appl Microbiol Biotechnol 64:763–781

    Article  PubMed  CAS  Google Scholar 

  5. Treichel H, de Oliveria D, Mazutti MA, Di Luccio M, Oliveria JV (2009) A review on microbial lipases production. Food Bioprocess Technol. doi:10.1007/s11947-009-0202-2

  6. Saxena RK, Davidson WS, Sheoran A, Giri B (2003) Purification and characterization on an alkaline thermostable lipase from Aspergillus carneus. Process Biochem 39:239–247

    Article  CAS  Google Scholar 

  7. Kaushik R, Saran S, Isar J, Saxena RK (2006) Statistical optimization of medium components and growth conditions by RSM to enhance lipase production by Aspergillus carneus. J Mol Catal B 40:121–126

    Article  CAS  Google Scholar 

  8. Gulati R, Saxena RK, Gupta R (2000) Fermentation and downstream processing on lipase from Aspergillus terreus. Process Biochem 36:149–155

    Article  CAS  Google Scholar 

  9. Winkler UK, Stuckman M (1979) Polysaccharide enhancement of exolipase formation by S. marcescens. J Bacteriol 138:663–670

    PubMed  CAS  Google Scholar 

  10. Haaland PD (1989) In: Haaland PD (ed) Experimental design in biotechnology. Marcel Dekker, New York, pp 1–8

  11. Elibol M, Ozer D (2002) Response surface analysis of lipase production by freely suspended Rhizopus arrhizus. Process Biochem 38:367–372

    Article  CAS  Google Scholar 

  12. Burkert JF, Maureri MF, Rodrigues MI (2004) Optimization of extracellular lipase production by Geotrichum sp. using factorial design. Bioresour Technol 91:77–84

    Article  PubMed  CAS  Google Scholar 

  13. Wang D, Xu Y, Shan T (2008) Effects of oils and oil-related substrates on the synthetic activity of membrane-bound lipase from Rhizopus cihinensis and optimization of the lipase fermentation media. Biochem Eng J 41:30–37

    Article  Google Scholar 

  14. Sharma A, Bardhan D, Patel R (2009) Optimization of physical parameters for lipase production from Arthrobacter sp. BGCC#490. Indian J Biochem Biophys 46:178–183

    PubMed  CAS  Google Scholar 

  15. Bornscheuer VT (1995) Lipase catalyzed synthesis of monoglycerols. Enzym Microb Technol 17:576–586

    Article  Google Scholar 

  16. Kwon SJ, Han JJ, Rhee JS (1995) Production and in situ separation of mono- or diacylglycerol catalyzed by lipases in n-hexane. Enzym Microb Technol 17:700–704

    Article  CAS  Google Scholar 

  17. Janssen AEM, Van der Padt A, Van Sonsbeek HM, Van’t Riet K (1993) The effect of organic solvents on the equilibrium position of enzymatic acylglycerol synthesis. Biotechnol Bioeng 41:95–103

    Article  CAS  Google Scholar 

  18. Ergan F, Trani M, Andre G (1990) Production of glycerides from glycerol and fatty acid by immobilized lipases in non-aqueous media. Biotechnol Bioeng 35:195–200

    Article  PubMed  CAS  Google Scholar 

  19. Xia YM, Fang Y, Zhang KC, Shi GY, Brown JJ (2003) Enzymatic synthesis of partial glycerol caprate in solvent-free media. J Mol Catal B 23:3–8

    Article  CAS  Google Scholar 

  20. Lou F-W, Liu B-K, Wu Q, Lv D-S, Lin X (2008) Candida antarctica lipase B (CAL-B)-catalyzed carbon-sulfur bond addition and controllable selectivity in organic media. Biotechnol Bioeng 80:200–225

    Google Scholar 

  21. Sabeder S, Habulin M, Knez Z (2005) Lipase-catalyzed synthesis of fatty acid fructose esters. J Food Eng 70:880–886

    Google Scholar 

  22. Arcos JA, Otero C, Hill CG (1998) Rapid enzymatic production of acyl glycerols from conjugated linoleic and glycerol in a solvent free system. Biotechnol Lett 20:617–621

    Article  CAS  Google Scholar 

Download references

Acknowledgment

Authors acknowledge with thanks the help of Ms. Pinki Anand for critically evaluating the manuscript.

Author information

Authors and Affiliations

  1. Department of Microbiology, University of Delhi, South Campus, New Delhi, 110021, India

    Rekha Kaushik, Ruchi Gulati Marwah, Pritesh Gupta, Saurabh Saran & R. K. Saxena

  2. Bioorganic Laboratory, Department of Chemistry, University of Delhi, Delhi, 110007, India

    V. S. Parmar

  3. Department of Human Physiology and Pharmacology, University of Rome, P. Le Aldo Moro 5, 00185, Rome, Italy

    Luciano Saso

Authors
  1. Rekha Kaushik
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ruchi Gulati Marwah
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pritesh Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Saurabh Saran
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Luciano Saso
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. V. S. Parmar
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. R. K. Saxena
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. K. Saxena.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kaushik, R., Marwah, R.G., Gupta, P. et al. Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions. Indian J Microbiol 50, 456–462 (2010). https://doi.org/10.1007/s12088-011-0100-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12088-011-0100-y

Keywords

Search

Navigation