Abstract
CCN3 is a matricellular protein that belongs to the CCN family. CCN3 consists of 4 domains: insulin-like growth factor-binding protein-like domain (IGFBP), von Willebrand type C-like domain (VWC), thrombospondin type 1-like domain (TSP1), and the C-terminal domain (CT) having a cysteine knot motif. Periostin is a secretory protein that binds to extracellular matrix proteins such as fibronectin and collagen. In this study, we found that CCN3 interacted with periostin. Immunoprecipitation analysis revealed that the TSP1-CT interacted with the 4 repeats of the Fas 1 domain of periostin. Immunofluorescence analysis showed co-localization of CCN3 and periostin in the periodontal ligament of mice. In addition, targeted disruption of the periostin gene in mice decreased the matricellular localization of CCN3 in the periodontal ligament. Thus, these results indicate that periostin was required for the matricellular localization of CCN3 in the periodontal ligament, suggesting that periostin mediated an interaction between CCN3 and the extracellular matrix.
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Abbreviations
- BMP:
-
Bone morphogenetic protein
- CT:
-
Carboxyl-terminal (C-terminal) domain
- EMI:
-
EMILIN
- Fas 1:
-
Fasciclin I
- IGFBP:
-
Insulin-like growth factor-binding protein
- TSP1:
-
Thrombospondin type 1
- VWC:
-
von Willebrand factor type C
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Acknowledgements
The authors thank Dr. H. Niwa (RIKEN, Japan) for the pCAGIPuro expression vector and Dr. B. Perbal (University of Paris) for K19 M antibody. This work was supported by grants-in-aid for scientific research from the Ministry of Education, Science, Culture, and Sports of Japan (KAKENHI Grant No. 19791362 (IK) and 19370093 (AK)) and partly by the Project for Cancer Research And Therapeutic Evolution (P-CREATE) (IK) from the Japan Agency for Medical Research and Development (AMED).
Author’s contributions
IT, HT, KK, and AK designed the research. IT, HT, HI, NA, and ML performed experiments. IT, HT, TN, and IK analyzed and interpreted the data. TN and IK wrote the manuscript.
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Issei Takayama, Hideyuki Tanabe and Takashi Nishiyama contributed equally.
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Takayama, I., Tanabe, H., Nishiyama, T. et al. Periostin is required for matricellular localization of CCN3 in periodontal ligament of mice. J. Cell Commun. Signal. 11, 5–13 (2017). https://doi.org/10.1007/s12079-016-0371-5
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DOI: https://doi.org/10.1007/s12079-016-0371-5