Abstract
We aimed to improve the diagnostic accuracy of tumors by using immunocytochemistry (IHC) on destained smears to correlate the cytological findings with histopathology and/or IHC for final evaluation of results. We have done a randomized and prospective double-blind study. Forty-six clinically suspected patients of salivary gland tumor were enrolled for study. Those with non-neoplastic\ inflammatory salivary gland pathologies and swellings other than salivary gland origin were excluded from the study. The Fine needle aspiration cytological (FNAC) diagnosis of all the patients was compared with the corresponding histopathology. Sensitivity and specificity of FNAC in diagnosing malignancy were 91.3 and 100 %. Diagnostic accuracy of FNAC for malignant tumors was 95.65 %. In our study kappa value came out to be 0.88 (p value <0.00), which indicated an excellent agreement between histological and cytological typing of salivary gland tumors. FNAC is a highly sensitive and specific technique for diagnosis of salivary gland tumors. Histopathology however is the gold standard for diagnosis, classification and grading of tumors. Immuno-cytochemistry can act as adjuvant in diagnosing salivary gland tumors, however it did not help in improving the diagnostic accuracy of FNAC in our study. Considering an excellent agreement between cytology and histology, a type specific diagnosis can be reached on FNAC of salivary gland tumors.
Similar content being viewed by others
References
Willian CF, Celeste NP (2004) Salivary gland cytopathology. Springer, New York
Cheuk W, Chan JKC (2004) Salivary gland tumors. In: Fletcher CDM (eds) Diagnostic histopathology of tumors, 2nd edn. Churchill Livingstone, Edinburgh, pp. 231–368
Ameena A, Afsar SS, Farrukh K (2010) Diagnostic reliability of FNAC for salivary gland swellings. Diagn Cytopathol 38:499–500
Shintani S, Matsura H, Hasegawa Y (1997) Fine needle aspiration of salivary gland tumors. Int J Oral Maxillofac Surg 26:284–286
Choudhary AA, Sultara T, Siddique BH, Amin ASA (2011) Diagnosis of parotid mass by the Fine Needle Aspiration Cytology (FNAC) and its histopathological correlation—2 year study in BSMMU, Dhaka. Bangabandhu Sheikh Mujib Med Univ J 4(2):65–69
Kumar S, Harish PS, Paramesha Kishan et al (2001) Role of FNAC of salivary gland tumor in correlation with their histopathology: a two year prospective study. JCDR 5(7):330–335
Pusztaszeri M, Braunschweig R, Mihaescu A (2009) Pleomorphic adenoma with predominant plasmocytoid myoepithelial cells: a diagnostic pitfall in aspiration cytology. Case report and review of the literature. Diagn Cytopathol 37(1):56–60
Kumar N, Kapila K, Verma K (1991) Fine needle aspiration cytology of mucoepidermoid carcinoma: a diagnostic problem. Acta Cytol 35:357–359
Kilijanicko J, Vielh P (1997) Fine needle aspiration sampling of salivary gland lesions II, cytology and histology correlation of 71 cases of Warthin’s tumor (Adenolymphoma). Diagn Cytopathol 16(3):221–225
Flezar M, Pogacink A (2002) Warthin’s tumor: unusual vs common morphological findings in fine needle aspiration biopsies. Cytopathol 13(4):232–241
Barnes L, Eveson JW, Reichart P, Sidransky D (2005) World Health Organization classification of tumors: pathology and genetics of head and neck tumors. IARC Press, Lyon
Kilijanicko J, Vielh P (1997) Fine needle sampling of salivary gland lesions IV. Review of 50 cases of mucoepidermoid carcinoma with histologic correlation. Diagn Cytopathol 17:92–98
Saenz-Santamaria J, Catalina Fernandez I, Fernandez Mera JJ, Villarreal Renedo P (2003) Low grade mucoepidermoid carcinoma arising in cervical lymph nodes. A report of two cases with fine needle aspiration findings. Acta Cytol 47(3):470–474
Klijaneinko J, Vielh P (1997) Fine needle sampling of salivary gland lesion III. Cytologic and histologic correlation of 75 cases of adenoid cystic carcinoma. Review and experience at the institute curie with emphasis on cytologic pitfalls. Diagn Cytopathol 17:36–41
Nagel H, Laskawi R, Buter JJ, Schroder M, Chilla R, Droese M (1997) Cytological diagnosis of acinic cell carcinoma of salivary glands. Diagn Cytopathol 16:402–412
Steward CJR, Mackenzie K, McGarry GW, Mowat A (2000) Fine needle aspiration cytology of salivary gland: a review of 341 cases. Diagn Cytopathol 22:139–146
Stramandinoli RT, Sassi LM, Pedruzzi PA, Ramos GA, Oliveira BV, Ogata DC, Sergoi-Ossamu I (2010) Accuracy, sensitivity and specificity of fine needle aspiration biopsy in salivary gland tumors: a retrospective study. Med Oral Pathol Oral Cir Bucal. 15(1):e32–e37
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that there is no conflict of interests.
Ethical Approval
This article does not contain any studies with animals performed by any of the authors. All procedures performed in this study involving human participant was in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed Consent
Informed consent was obtained from the participants included in the study.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Sarkar, A., Sharma, N. & Sharma, S. Fine Needle Aspiration Cytology Utility in Salivary Gland Tumor Diagnosis. Indian J Otolaryngol Head Neck Surg 69, 147–154 (2017). https://doi.org/10.1007/s12070-016-0982-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12070-016-0982-4