Abstract
The aim of this study was to compare the cytokine levels in nasal fluid in subjects with nasal polyposis (NP) and co-morbid asthma and NP patients without asthma and to correlate these levels with clinical parameters of severity of disease. Forty NP patients (20 asthmatic and 20 nonasthmatic) were enrolled. Nasal secretion samples were collected from nasal cavities of all 40 subjects. The levels of Th1 cytokines IL-2, IL-12 and IFN-γ, Th2 cytokines IL-4, IL-5, IL-6, and IL-10, chemokine IL-8, and proinflammatory cytokines IL-1β, TNF-α and TNF-β were measured using flow cytometric method. Each of the 40 patients was staged clinically according to global nasal symptom score, endoscopic score, and Lund-Mackay computed tomography (CT) score. The concentrations of Th2 cytokines IL-5, IL-6 and IL-10 were significantly higher (P < 0.01, P < 0.01, P < 0.05) in patients with NP and asthma compared with NP patients without asthma. Positive correlations were observed between concentration of IL-2 in nasal secretions and global nasal symptom score, endoscopic score, and Lund-Mackay score only in NP patients without asthma. We also found positive correlation between CT score and the levels of IL-8, IL-4, and IL-1β in nonasthmatic patients. Finally, our results showed a positive correlation between IL-5 levels in nasal fluid and endoscopic score only in asthmatic patients. NP in asthmatic patients have different immunological patterns compared to those without asthma. We also concluded that concentrations of cytokines measured in nasal fluid were not sensitive enough to determine the severity of disease.
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Rinia AB, Kostamo K, Ebbens FA, van Drunen CM, Fokkens WJ (2007) Nasal polyposis: a cellular-based approach to answering questions. Allergy 62:348–358
Fokkens W, Lund V (2007) European posistion paper on rhinosinusitis and nasal polyps. Rhinol Suppl 45(Suppl 20):1–136
Erbek SS, Yurtcu E, Erbek S, Atac FB, Sahin FI, Cakmak O (2007) Proinflammatory cytokine single nucleotide polymorphisms in nasal polyposis. Arch Otolaryngol Head Neck Surg 133:705–709
Ramanathan M, Lee WK, Spannhake EW, Lane AP (2008) Th2 cytokines associated with chronic rhinosinusitis with polyps down-regulate the antimicrobial immune function of human sinonasal epithelial cells. Am J Rhinol 22:115–121
Larsen K (1996) The clinical relationship of nasal polyps to asthma. Allergy Asthma Proc 17:243–249
Král B, Krejsek J, Paráková Z, Kopecký O, Vokurková D, Derner V et al (1997) Some serum activity markers of airways inflammation in difficult-to-control asthma patients. Acta Medica (Hradec Králové) 40:61–70
Dunđerski J, Matić G (2009) Glucocorticoid receptor in health and disease. J Med Biochem 28:248–261
Jankowski R, Persoons M, Foliguet B, Coffinet L, Thomas C, Verient-Montaut B (2000) Eosinophil count in nasal secretions of subjects with and without nasal symptoms. Rhinology 38:23–32
Riechelmann H, Deutschle T, Rozsasi A, Keck T, Polzehl D, Bürner H (2005) Nasal biomarker profiles in acute and chronic rhinosinusitis. Clin Exp Allergy 35(9):1186–1191
Riechelmann H, Deutschle T, Friemel E, Gross HJ, Bachem M (2003) Biological markers in nasal secretions. Eur Respir J 21:600–605
Ohkubo K, Masakazu I, Pawankar R, Gotoh M, Yagi T, Okuda M (1998) Mechanisms of IL-6, IL-8 and GM-CSF release in nasal secretions of allergic patients after nasal challenge. Rhinology 36:156–161
Bousquet J (2000) Global Initiative for Asthma (GINA) and its objectives. Clin Exp Allergy 30(Suppl 1):2–5
Tsicopoulos A, Shimbara A, de Nadai P, Aldewachi O, Lamblin C, Lassalle P et al (2004) Involvement of IL-9 in the bronchial phenotype of patients with nasal polyposis. J Allergy Clin Immunol 113:462–469
Lildholdt T, Rundcrantz H, Bende M, Larsen K (1997) Glucocorticoid treatment for nasal polyps: the use of topical budesonide powder, intramuscular betamethasone, and surgical treatment. Arch Otolaryngol Head Neck Surg 123:595–600
Lund VJ, Mackay IS (1993) Staging in rhinosinusitis. Rhinology 31:183–184
Watelet JB, Gevaert P, Holtappels G, Van Cauwenberge P, Bachert C (2004) Collection of nasal secretions for immunological analysis. Eur Arch Otorhinolaryngol 261:242–246
Bachert C, Van Kempen M, Van Cauwenberge P (1999) Regulation of proinflammatory cytokines in seasonal allergic rhinitis. Int Arch Allergy Immunol 118:375–379
Hens G, Hellings PW (2006) The nose: gatekeeper and trigger of bronchial disease. Rhinology 44:179–187
Perić A, Gaćeša D (2008) Etiology and pathogenesis of chronic rhinosinusitis. Vojnosanit Pregl 65:699–702
Hamilos DL, Leung DJ, Wood R, Cunningham L, Bean DK, Yasruel Z et al (1995) Evidence for distinct cytokine expression in allergic versus nonallergic chronic sinusitis. J Allergy Clin Immunol 96:537–544
Bachert C, Wagenmann M, Rudack C, Höpken K, Hillebrandt M, Wang D et al (1998) The role of cytokines in infectious sinusitis and nasal polyposis. Allergy 53:2–13
Fan GK, Wang H, Takenaka H (2007) Eosinophil infiltration and activation in nasal polyposis. Acta Otolaryngol 127:521–526
Liu CM, Hong CY, Shun CT, Hsiao TY, Wang CC, Wang JS et al (2002) Inducible cyclooxygenase and interleukin-6 gene expression in nasal polyp fibroblasts. Arch Otolaryngol Head Neck Surg 128:945–951
Van Zele T, Gevaert P, Watelet JB, Claeys G, Holtappels G, Claeys C et al (2004) Staphylococcus aureus colonization and IgE antibody formation to enterotoxins is increased in nasal polyposis. J Allergy Clin Immunol 114:981–983
Hellings PW, Hens G, Meyts I, Bullens D, Vanoirbeek J, Gevaert P et al (2006) Aggravation of bronchial eosinophilia in mice by nasal and bronchial exposure to Staphylococcus aureus enterotoxin B. Clin Exp Allergy 36:1063–1071
Chen YS, Arab SF, Westhofen M, Lorenzen J (2005) Expression of interleukin-5, interleukin-8, and interleukin-10 mRNA in the ostiomeatal complex in nasal polyposis. Am J Rhinol 19:117–123
Yoshifuku K, Matsune S, Ohori J, Sagara Y, Fukuiwa T, Kurono Y (2007) IL-4 and TNF-α increased the secretion of eotaxin from cultured fibroblasts of nasal polyps with eosinophil infiltration. Rhinology 45:235–241
Saji F, Nonaka M, Pawankar R (2000) Expression of RANTES by IL-1β and TNF-α stimulated nasal polyp fibroblasts. Auris Nasus Larynx 27:247–252
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Perić, A., Vojvodić, D., Perić, A.V. et al. Correlation Between Cytokine Levels in Nasal Fluid and Scored Clinical Parameters in Patients with Nasal Polyposis. Indian J Otolaryngol Head Neck Surg 65 (Suppl 2), 295–300 (2013). https://doi.org/10.1007/s12070-011-0447-8
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DOI: https://doi.org/10.1007/s12070-011-0447-8